{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T13:59:31Z","timestamp":1722175171620},"reference-count":33,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Surface antigen variation in\n            <jats:italic>Mycoplasma agalactiae<\/jats:italic>\n            , the etiologic agent of contagious agalactia in sheep and goats, is governed by site-specific recombination within the\n            <jats:italic>vpma<\/jats:italic>\n            multigene locus encoding the Vpma family of variable surface lipoproteins. This high-frequency Vpma phase switching was previously shown to be mediated by a Xer1 recombinase encoded adjacent to the\n            <jats:italic>vpma<\/jats:italic>\n            locus. In this study, it was demonstrated in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            that the Xer1 recombinase is responsible for catalyzing\n            <jats:italic>vpma<\/jats:italic>\n            gene inversions between recombination sites (RS) located in the 5\u2032-untranslated region (UTR) in all six\n            <jats:italic>vpma<\/jats:italic>\n            genes, causing cleavage and strand exchange within a 21-bp conserved region that serves as a recognition sequence. It was further shown that the outcome of the site-specific recombination event depends on the orientation of the two\n            <jats:italic>vpma<\/jats:italic>\n            RS, as direct or inverted repeats. While recombination between inverted\n            <jats:italic>vpma<\/jats:italic>\n            RS led to inversions, recombination between direct repeat\n            <jats:italic>vpma<\/jats:italic>\n            RS led to excisions. Using a newly developed excision assay based on the\n            <jats:italic>lacZ<\/jats:italic>\n            reporter system, we were able to successfully demonstrate under native conditions that such Xer1-mediated excisions can indeed also occur in the\n            <jats:italic>M. agalactiae<\/jats:italic>\n            type strain PG2, whereas they were not observed in the control\n            <jats:italic>xer1<\/jats:italic>\n            -disrupted VpmaY phase-locked mutant (PLMY), which lacks Xer1 recombinase. Unless there are specific regulatory mechanisms preventing such excisions, this might be the cost that the pathogen has to render at the population level for maintaining this high-frequency phase variation machinery.\n          <\/jats:p>","DOI":"10.1128\/jb.01537-09","type":"journal-article","created":{"date-parts":[[2010,6,21]],"date-time":"2010-06-21T00:23:18Z","timestamp":1277079798000},"page":"4462-4473","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Xer1-Mediated Site-Specific DNA Inversions and Excisions in\n            <i>Mycoplasma agalactiae<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Stefan","family":"Czurda","sequence":"first","affiliation":[{"name":"Institute of Bacteriology, Mycology and Hygiene (IBMH), Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Jechlinger","sequence":"additional","affiliation":[{"name":"Institute of Bacteriology, Mycology and Hygiene (IBMH), Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renate","family":"Rosengarten","sequence":"additional","affiliation":[{"name":"Institute of Bacteriology, Mycology and Hygiene (IBMH), Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohini","family":"Chopra-Dewasthaly","sequence":"additional","affiliation":[{"name":"Institute of Bacteriology, Mycology and Hygiene (IBMH), Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"149","year":"2002","unstructured":"Barre, F. 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