{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:18:54Z","timestamp":1791411534724,"version":"4.3.3"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>CsrA is a global posttranscriptional regulator of numerous physiological processes, such as glycogenesis and glycolysis. Here, we show that the<jats:italic>csrA<\/jats:italic>gene of<jats:italic>Escherichia coli<\/jats:italic>is essential for growth on LB and on synthetic medium containing glycolytic carbon sources. However,<jats:italic>csrA<\/jats:italic>is not necessary for growth on synthetic medium containing pyruvate, showing that the Krebs cycle is functional in the<jats:italic>csrA<\/jats:italic>::<jats:italic>cat<\/jats:italic>deletion mutant. Deletion of the<jats:italic>glgCAP<\/jats:italic>operon in the<jats:italic>csrA<\/jats:italic>::<jats:italic>cat<\/jats:italic>mutant restored the ability to grow on LB and on synthetic medium containing glycolytic carbon sources, showing that growth inhibition is due to an excess of glycogen synthesis.<\/jats:p>","DOI":"10.1128\/jb.01573-08","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T01:58:20Z","timestamp":1229997500000},"page":"1722-1724","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Conditional Essentiality of the<i>csrA<\/i>Gene in<i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"191","author":[{"given":"Johan","family":"Timmermans","sequence":"first","affiliation":[{"name":"Laboratoire de Ge\u0301ne\u0301tique et de Physiologie Bacte\u0301rienne, Institut de Biologie et de Me\u0301decine Mole\u0301culaires, Faculte\u0301 des Sciences, Universite\u0301 Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, B:6041 Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Van Melderen","sequence":"additional","affiliation":[{"name":"Laboratoire de Ge\u0301ne\u0301tique et de Physiologie Bacte\u0301rienne, Institut de Biologie et de Me\u0301decine Mole\u0301culaires, Faculte\u0301 des Sciences, Universite\u0301 Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, B:6041 Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.mib.2007.03.007","volume":"10","year":"2007","unstructured":"Babitzke, P., and T. Romeo. 2007. CsrB sRNA family: sequestration of RNA-binding regulatory proteins. Curr. Opin. Microbiol. 10 : 156-163.","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1046\/j.1365-2958.2002.02982.x","volume":"44","year":"2002","unstructured":"Baker, C. S., I. Morozov, K. Suzuki, T. Romeo, and P. Babitzke. 2002. CsrA regulates glycogen biosynthesis by preventing translation of glgC in Escherichia coli. Mol. Microbiol. 44 : 1599-1610.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1046\/j.1365-2958.2003.03788.x","volume":"51","year":"2004","unstructured":"Barnard, F. M., M. F. Loughlin, H. P. Fainberg, M. P. Messenger, D. W. Ussery, P. Williams, and P. J. Jenks. 2004. Global regulation of virulence and the stress response by CsrA in the highly adapted human gastric pathogen Helicobacter pylori. Mol. Microbiol. 51 : 15-32.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"6929","DOI":"10.1073\/pnas.81.22.6929","volume":"81","year":"1984","unstructured":"Brosius, J., and A. Holy. 1984. Regulation of ribosomal RNA promoters with a synthetic lac operator. Proc. Natl. Acad. Sci. USA 81 : 6929-6933.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.17.5108-5115.1995"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.15.4450-4460.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01928-07"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ijmm.2003.12.003","volume":"294","year":"2004","unstructured":"Forsbach-Birk, V., T. McNealy, C. Shi, D. Lynch, and R. Marre. 2004. Reduced expression of the global regulator protein CsrA in Legionella pneumophila affects virulence-associated regulators and growth in Acanthamoeba castellanii. Int. J. Med. Microbiol. 294 : 15-25.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1093\/genetics\/51.2.167","volume":"51","year":"1965","unstructured":"Glansdorff, N. 1965. Topography of cotransducible arginine mutations in Escherichia coli K-12. Genetics 51 : 167-179.","journal-title":"Genetics"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4121-4130.1995"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1046\/j.1365-2958.2003.03533.x","volume":"48","year":"2003","unstructured":"Hansen, A. M., H. Lehnherr, X. Wang, V. Mobley, and D. J. Jin. 2003. Escherichia coli SspA is a transcription activator for bacteriophage P1 late genes. Mol. Microbiol. 48 : 1621-1631.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1111\/j.1365-2958.2008.06218.x","volume":"68","year":"2008","unstructured":"Heroven, A. K., K. Bohme, M. Rohde, and P. Dersch. 2008. A Csr-type regulatory system, including small non-coding RNAs, regulates the global virulence regulator RovA of Yersinia pseudotuberculosis through RovM. Mol. Microbiol. 68 : 1179-1195.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.1.290-301.2002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1365-2958.2007.06042.x","volume":"67","year":"2008","unstructured":"Lapouge, K., M. Schubert, F. H. Allain, and D. Haas. 2008. Gac\/Rsm signal transduction pathway of gamma-proteobacteria: from RNA recognition to regulation of social behaviour. Mol. Microbiol. 67 : 241-253.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1111\/j.1365-2958.2005.04902.x","volume":"58","year":"2005","unstructured":"Lenz, D. H., M. B. Miller, J. Zhu, R. V. Kulkarni, and B. L. Bassler. 2005. CsrA and three redundant small RNAs regulate quorum sensing in Vibrio cholerae. Mol. Microbiol. 58 : 1186-1202.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1099\/jmm.0.05024-0","volume":"52","year":"2003","unstructured":"Liaw, S. J., H. C. Lai, S. W. Ho, K. T. Luh, and W. B. Wang. 2003. Role of RsmA in the regulation of swarming motility and virulence factor expression in Proteus mirabilis. J. Med. Microbiol. 52 : 19-28.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.14.4639-4642.1997"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.10.2663-2672.1995"},{"key":"e_1_3_2_21_2","first-page":"31832","volume":"281","year":"2006","unstructured":"Mercante, J., K. Suzuki, X. Cheng, P. Babitzke, and T. Romeo. 2006. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J. Biol. Chem. 281 : 31832-31842.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.15.4744-4755.1993"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"29096","DOI":"10.1074\/jbc.270.49.29096","volume":"270","year":"1995","unstructured":"Sabnis, N. A., H. Yang, and T. Romeo. 1995. Pleiotropic regulation of central carbohydrate metabolism in Escherichia coli via the gene csrA. J. Biol. Chem. 270 : 29096-29104.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1038\/nsmb1285","volume":"14","year":"2007","unstructured":"Schubert, M., K. Lapouge, O. Duss, F. C. Oberstrass, I. Jelesarov, D. Haas, and F. H. Allain. 2007. Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA\/CsrA. Nat. Struct. Mol. Biol. 14 : 807-813.","journal-title":"Nat. Struct. Mol. Biol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.24.7257-7265.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0378-1119(91)90366-J","volume":"100","year":"1991","unstructured":"Wang, R. F., and S. R. Kushner. 1991. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100 : 195-199.","journal-title":"Gene"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1111\/j.1365-2958.2005.04648.x","volume":"56","year":"2005","unstructured":"Wang, X., A. K. Dubey, K. Suzuki, C. S. Baker, P. Babitzke, and T. Romeo. 2005. CsrA post-transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli. Mol. Microbiol. 56 : 1648-1663.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.6.1632-1640.2000"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1046\/j.1365-2958.2001.02380.x","volume":"40","year":"2001","unstructured":"Wei, B. L., A. M. Brun-Zinkernagel, J. W. Simecka, B. M. Pruss, P. Babitzke, and T. Romeo. 2001. Positive regulation of motility and flhDC expression by the RNA-binding protein CsrA of Escherichia coli. Mol. Microbiol. 40 : 245-256.","journal-title":"Mol. Microbiol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01573-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01573-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T11:51:50Z","timestamp":1709812310000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.01573-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1128\/JB.01573-08"],"URL":"https:\/\/doi.org\/10.1128\/jb.01573-08","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3]]},"assertion":[{"value":"2008-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-12-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}