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The lack of methods to genetically manipulate\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    significantly impedes the study of this organism. We describe here the cloning and characterization of a\n                    <jats:italic>C. burnetii ftsZ<\/jats:italic>\n                    mutant generated by\n                    <jats:italic>mariner<\/jats:italic>\n                    -based\n                    <jats:italic>Himar1<\/jats:italic>\n                    transposon (Tn) mutagenesis.\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    was coelectroporated with a plasmid encoding the\n                    <jats:italic>Himar1<\/jats:italic>\n                    C9 transposase variant and a plasmid containing a\n                    <jats:italic>Himar1<\/jats:italic>\n                    transposon encoding chloramphenicol acetyltransferase, mCherry fluorescent protein, and a ColE1 origin of replication. Vero cells were infected with electroporated\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    and transformants scored as organisms replicating in the presence of chloramphenicol and expressing mCherry. Southern blot analysis revealed multiple transpositions in the\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    genome and rescue cloning identified 30 and 5 insertions in coding and noncoding regions, respectively. Using micromanipulation, a\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    clone was isolated containing a Tn insertion within the C terminus of the cell division gene\n                    <jats:italic>ftsZ<\/jats:italic>\n                    . The\n                    <jats:italic>ftsZ<\/jats:italic>\n                    mutant had a significantly lower growth rate than wild-type bacteria and frequently appeared as filamentous forms displaying incomplete cell division septa. The latter phenotype correlated with a deficiency in generating infectious foci on a per-genome basis compared to wild-type organisms. The mutant FtsZ protein was also unable to bind the essential cell division protein FtsA. This is the first description of\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    harboring a defined gene mutation generated by genetic transformation.\n                  <\/jats:p>","DOI":"10.1128\/jb.01580-08","type":"journal-article","created":{"date-parts":[[2008,12,29]],"date-time":"2008-12-29T22:50:13Z","timestamp":1230591013000},"page":"1369-1381","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":108,"title":["Characterization of a\n                    <i>Coxiella burnetii ftsZ<\/i>\n                    Mutant Generated by\n                    <i>Himar1<\/i>\n                    Transposon Mutagenesis"],"prefix":"10.1128","volume":"191","author":[{"given":"Paul A.","family":"Beare","sequence":"first","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale","family":"Howe","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane C.","family":"Cockrell","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Omsland","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Hansen","sequence":"additional","affiliation":[{"name":"Electron Microscopy Facility, Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Heinzen","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"3681","DOI":"10.1093\/nar\/22.18.3681","volume":"22","year":"1994","unstructured":"Bakin, A., J. 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