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The metabolic activity of butyrate-oxidizing\n            <jats:italic>S. wolfei<\/jats:italic>\n            cells was measured via production of formazan and acetate from butyrate, with 2,3,5-triphenyltetrazolium chloride as electron acceptor. This activity was inhibited by trifluoperazine (TPZ), an antitubercular agent known to inhibit NADH:menaquinone oxidoreductase. In cell extracts of\n            <jats:italic>S. wolfei<\/jats:italic>\n            , the oxidation of NADH could be measured with quinones, viologens, and tetrazolium dyes as electron acceptors, and also this activity was inhibited by TPZ. The TPZ-sensitive NADH:acceptor oxidoreductase activity appeared to be membrane associated but could be dissociated from the membrane as a soluble protein and was semipurified by anion-exchange chromatography. Recovered proteins were identified by peptide mass fingerprinting, which indicated the presence of an NADH:acceptor oxidoreductase as part of a three-component [FeFe] hydrogenase complex and a selenocysteine-containing formate dehydrogenase. Furthermore, purification of butyryl-CoA dehydrogenase (Bcd) activity and peptide mass fingerprinting revealed two Bcd proteins different from the Bcd subunit of the Bcd\/electron-transfer flavoprotein complex (Bcd\/EtfAB) predicted from the genome sequence of\n            <jats:italic>S. wolfei<\/jats:italic>\n            . The results suggest that syntrophic oxidation of butyrate in\n            <jats:italic>S. wolfei<\/jats:italic>\n            involves a membrane-associated TPZ-sensitive NADH:acceptor oxidoreductase as part of a hydrogenase complex similar to the recently discovered \u201cbifurcating\u201d hydrogenase in\n            <jats:italic>Thermotoga maritima<\/jats:italic>\n            and butyryl-CoA dehydrogenases that are different from Bcd of the Bcd\/EtfAB complex.\n          <\/jats:p>","DOI":"10.1128\/jb.01605-08","type":"journal-article","created":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T01:10:02Z","timestamp":1249089002000},"page":"6167-6177","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Involvement of NADH:Acceptor Oxidoreductase and Butyryl Coenzyme A Dehydrogenase in Reversed Electron Transport during Syntrophic Butyrate Oxidation by\n            <i>Syntrophomonas wolfei<\/i>"],"prefix":"10.1128","volume":"191","author":[{"given":"Nicolai","family":"Mu\u0308ller","sequence":"first","affiliation":[{"name":"Fachbereich Biologie, Universita\u0308t Konstanz, D-78457 Constance, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Schleheck","sequence":"additional","affiliation":[{"name":"Fachbereich Biologie, Universita\u0308t Konstanz, D-78457 Constance, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Schink","sequence":"additional","affiliation":[{"name":"Fachbereich Biologie, Universita\u0308t Konstanz, D-78457 Constance, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1267\/ahc.9.1","volume":"9","year":"1976","unstructured":"Altman, F. 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