{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:01:56Z","timestamp":1790805716130,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2014,8,15]],"date-time":"2014-08-15T00:00:00Z","timestamp":1408060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2014,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            biology is imperfectly understood, and the nutrients available in the intracellular niche are unknown. Here, we investigated the central pathways of C metabolism used by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella abortus<\/jats:named-content>\n            by deleting the putative fructose-1,6-bisphosphatase (\n            <jats:italic>fbp<\/jats:italic>\n            and\n            <jats:italic>glpX<\/jats:italic>\n            ), phosphoenolpyruvate carboxykinase (\n            <jats:italic>pckA<\/jats:italic>\n            ), pyruvate phosphate dikinase (\n            <jats:italic>ppdK<\/jats:italic>\n            ), and malic enzyme (\n            <jats:italic>mae<\/jats:italic>\n            ) genes. In gluconeogenic but not in rich media, growth of \u0394\n            <jats:italic>ppdK<\/jats:italic>\n            and \u0394\n            <jats:italic>mae<\/jats:italic>\n            mutants was severely impaired and growth of the double \u0394\n            <jats:italic>fbp<\/jats:italic>\n            -\u0394\n            <jats:italic>glpX<\/jats:italic>\n            mutant was reduced. In macrophages, only the \u0394\n            <jats:italic>ppdK<\/jats:italic>\n            and \u0394\n            <jats:italic>mae<\/jats:italic>\n            mutants showed reduced multiplication, and studies with the \u0394\n            <jats:italic>ppdK<\/jats:italic>\n            mutant confirmed that it reached the replicative niche. Similarly, only the \u0394\n            <jats:italic>ppdK<\/jats:italic>\n            and \u0394\n            <jats:italic>mae<\/jats:italic>\n            mutants were attenuated in mice, the former being cleared by week 10 and the latter persisting longer than 12 weeks. We also investigated the glyoxylate cycle. Although\n            <jats:italic>aceA<\/jats:italic>\n            (isocitrate lyase) promoter activity was enhanced in rich medium,\n            <jats:italic>aceA<\/jats:italic>\n            disruption had no effect\n            <jats:italic>in vitro<\/jats:italic>\n            or on multiplication in macrophages or mouse spleens. The results suggest that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            grows intracellularly using a limited supply of 6-C (and 5-C) sugars that is compensated by glutamate and possibly other amino acids entering the Krebs cycle without a critical role of the glyoxylate shunt.\n          <\/jats:p>","DOI":"10.1128\/jb.01663-14","type":"journal-article","created":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T02:43:53Z","timestamp":1402973033000},"page":"3045-3057","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Brucella abortus Depends on Pyruvate Phosphate Dikinase and Malic Enzyme but Not on Fbp and GlpX Fructose-1,6-Bisphosphatases for Full Virulence in Laboratory Models"],"prefix":"10.1128","volume":"196","author":[{"given":"Amaia","family":"Z\u00fa\u00f1iga-Ripa","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibault","family":"Barbier","sequence":"additional","affiliation":[{"name":"Research Unit in Biology of Microorganisms\u2014URBM, NARILIS, UNAmur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Conde-\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estrella","family":"Mart\u00ednez-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyre","family":"Palacios-Chaves","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Gil-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jes\u00fas","family":"Grill\u00f3","sequence":"additional","affiliation":[{"name":"Grupo de Sanidad Animal, Instituto de Agrobiotecnolog\u00eda (CSIC-Universidad P\u00fablica de Navarra-Gobierno de Navarra), Campus de Arrosad\u00eda, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Research Unit in Biology of Microorganisms\u2014URBM, NARILIS, UNAmur, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maite","family":"Iriarte","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Moriy\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Instituto de Salud Tropical, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000631"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002675"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20120156"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2012.11.005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5711-5724.1998"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00718.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.08.011"},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/0-387-30745-1_17","volume-title":"The prokaryotes: a handbook on the biology of bacteria","author":"Moreno E","year":"2006","unstructured":"MorenoEMoriy\u00f3nI. 2006. 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