{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:31:20Z","timestamp":1791325880940,"version":"4.3.1"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Legionella pneumophila<\/jats:named-content>\n is a Gram-negative freshwater agent which multiplies in specialized nutrient-rich vacuoles of amoebae. When replicating in human alveolar macrophages,\n Legionella<\/jats:named-content>\n can cause Legionnaires' disease. Recently, we identified a new type of conjugation\/type IVA secretion system (T4ASS) in\n L. pneumophila<\/jats:named-content>\n Corby (named\n trb<\/jats:italic>\n -\n tra<\/jats:italic>\n ). Analogous versions of\n trb<\/jats:italic>\n -\n tra<\/jats:italic>\n are localized on the genomic islands Trb-1 and Trb-2. Both can exist as an episomal circular form, and Trb-1 can be transferred horizontally to other\n Legionella<\/jats:named-content>\n strains by conjugation. In our current work, we discovered the importance of a site-specific integrase (Int-1,\n lpc2818<\/jats:italic>\n ) for the excision and conjugation process of Trb-1. Furthermore, we identified the genes\n lvrRABC<\/jats:italic>\n (\n lpc2813<\/jats:italic>\n to\n lpc2816<\/jats:italic>\n ) to be involved in the regulation of Trb-1 excision. In addition, we demonstrated for the first time that a\n Legionella<\/jats:named-content>\n genomic island (LGI) of\n L. pneumophila<\/jats:named-content>\n Corby (LpcGI-2) encodes a functional type IV secretion system. The island can be transferred horizontally by conjugation and is integrated site specifically into the genome of the transconjugants. LpcGI-2 generates three different episomal forms. The predominant episomal form, form A, is generated integrase dependently (Lpc1833) and transferred by conjugation in a\n pilT<\/jats:italic>\n -dependent manner. Therefore, the genomic islands Trb-1 and LpcGI-2 should be classified as integrative and conjugative elements (ICEs). Coculture studies of\n L. pneumophila<\/jats:named-content>\n wild-type and mutant strains revealed that the\n int-1<\/jats:italic>\n and\n lvrRABC<\/jats:italic>\n genes (located on Trb-1) as well as\n lpc1833<\/jats:italic>\n and\n pilT<\/jats:italic>\n (located on LpcGI-2) do not influence the\n in vivo<\/jats:italic>\n fitness of\n L. pneumophila<\/jats:named-content>\n in\n Acanthamoeba castellanii<\/jats:named-content>\n .\n <\/jats:p>","DOI":"10.1128\/jb.01739-12","type":"journal-article","created":{"date-parts":[[2013,1,26]],"date-time":"2013-01-26T02:51:45Z","timestamp":1359168705000},"page":"1583-1597","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Regulation, Integrase-Dependent Excision, and Horizontal Transfer of Genomic Islands in Legionella pneumophila"],"prefix":"10.1128","volume":"195","author":[{"given":"Monika","family":"Lautner","sequence":"first","affiliation":[{"name":"Centre for Biological Security, Division 2 (ZBS2), Cellular Interactions of Bacterial Pathogens Working Group, Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Schunder","sequence":"additional","affiliation":[{"name":"Centre for Biological Security, Division 2 (ZBS2), Cellular Interactions of Bacterial Pathogens Working Group, Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vroni","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Centre for Biological Security, Division 2 (ZBS2), Cellular Interactions of Bacterial Pathogens Working Group, Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Heuner","sequence":"additional","affiliation":[{"name":"Centre for Biological Security, Division 2 (ZBS2), Cellular Interactions of Bacterial Pathogens Working Group, Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.33.12.1179"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10041-X"},{"key":"e_1_3_3_4_2","first-page":"678","article-title":"Current views on the relationships between amoebae, legionellae and man","volume":"22","author":"Rowbotham TJ","year":"1986","unstructured":"RowbothamTJ. 1986. 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