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In this study, we examined the\n            <jats:italic>Corynebacterium hmu<\/jats:italic>\n            hemin transport region, a genetic cluster that contains the\n            <jats:italic>hmuTUV<\/jats:italic>\n            genes encoding a previously described ABC-type hemin transporter and three additional genes, which we have designated\n            <jats:italic>htaA<\/jats:italic>\n            ,\n            <jats:italic>htaB<\/jats:italic>\n            , and\n            <jats:italic>htaC<\/jats:italic>\n            . The\n            <jats:italic>hmu<\/jats:italic>\n            gene cluster is composed of three distinct transcriptional units. The\n            <jats:italic>htaA<\/jats:italic>\n            gene appears to be part of an iron- and DtxR-regulated operon that includes\n            <jats:italic>hmuTUV<\/jats:italic>\n            , while\n            <jats:italic>htaB<\/jats:italic>\n            and\n            <jats:italic>htaC<\/jats:italic>\n            are transcribed from unique DtxR-regulated promoters. Nonpolar deletion of either\n            <jats:italic>htaA<\/jats:italic>\n            or the\n            <jats:italic>hmuTUV<\/jats:italic>\n            genes resulted in a reduced ability to use hemin as an iron source, while deletion of\n            <jats:italic>htaB<\/jats:italic>\n            had no effect on hemin iron utilization in\n            <jats:italic>C. diphtheriae<\/jats:italic>\n            . A comparison of the predicted amino acid sequences of HtaA and HtaB showed that they share some sequence similarity, and both proteins contain leader sequences and putative C-terminal transmembrane regions. Protein localization studies with\n            <jats:italic>C. diphtheriae<\/jats:italic>\n            showed that HtaA is associated predominantly with the cell envelope when the organism is grown in minimal medium but is secreted during growth in nutrient-rich broth. HtaB and HmuT were detected primarily in the cytoplasmic membrane fraction regardless of the growth medium. Hemin binding studies demonstrated that HtaA and HtaB are able to bind hemin, suggesting that these proteins may function as cell surface hemin receptors in\n            <jats:italic>C. diphtheriae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.01784-08","type":"journal-article","created":{"date-parts":[[2009,2,7]],"date-time":"2009-02-07T09:01:19Z","timestamp":1233997279000},"page":"2638-2648","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["HtaA Is an Iron-Regulated Hemin Binding Protein Involved in the Utilization of Heme Iron in\n            <i>Corynebacterium diphtheriae<\/i>"],"prefix":"10.1128","volume":"191","author":[{"given":"Courtni E.","family":"Allen","sequence":"first","affiliation":[{"name":"Laboratory of Respiratory and Special Pathogens, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Laboratory of Respiratory and Special Pathogens, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.67.2.220-232.1954"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1042-1055.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"5968","DOI":"10.1073\/pnas.87.15.5968","volume":"87","year":"1990","unstructured":"Boyd, J. 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