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In this study, the role of AlgR in biofilms was examined in continuous-flow and static biofilm assays. Strain PSL317 (\u0394<jats:italic>algR<\/jats:italic>) produced one-third the biofilm biomass of wild-type strain PAO1. Complementation with<jats:italic>algR<\/jats:italic>, but not<jats:italic>fimTU-pilVWXY1Y2E<\/jats:italic>, restored PSL317 to the wild-type biofilm phenotype. Comparisons of the transcriptional profiles of biofilm-grown PAO1 and PSL317 revealed that a number of quorum-sensing genes were upregulated in the<jats:italic>algR<\/jats:italic>deletion strain. Measurement of<jats:italic>rhlA<\/jats:italic>::<jats:italic>lacZ<\/jats:italic>and<jats:italic>rhlI<\/jats:italic>::<jats:italic>lacZ<\/jats:italic>promoter fusions confirmed the transcriptional profiling data when PSL317 was grown as a biofilm, but not planktonically. Increased amounts of rhamnolipids and<jats:italic>N<\/jats:italic>-butyryl homoserine lactone were detected in the biofilm effluent but not the planktonic supernatants of the<jats:italic>algR<\/jats:italic>mutant. Additionally, AlgR specifically bound to the<jats:italic>rhlA<\/jats:italic>and<jats:italic>rhlI<\/jats:italic>promoters in mobility shift assays. Moreover, PAO1 containing a chromosomal mutated AlgR binding site in its<jats:italic>rhlI<\/jats:italic>promoter formed biofilms and produced increased amounts of rhamnolipids similarly to the<jats:italic>algR<\/jats:italic>deletion strain. These observations indicate that AlgR specifically represses the Rhl quorum-sensing system during biofilm growth and that such repression is necessary for normal biofilm development. These data also suggest that AlgR may control transcription in a contact-dependent or biofilm-specific manner.<\/jats:p>","DOI":"10.1128\/jb.01797-06","type":"journal-article","created":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:53:59Z","timestamp":1188608039000},"page":"7752-7764","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["<i>Pseudomonas aeruginosa<\/i>AlgR Represses the Rhl Quorum-Sensing System in a Biofilm-Specific Manner"],"prefix":"10.1128","volume":"189","author":[{"given":"Lisa A.","family":"Morici","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane University Health Sciences Center, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Carterson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane University Health Sciences Center, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria E.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, Rochester, New York 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Frisk","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane University Health Sciences Center, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill R.","family":"Schurr","sequence":"additional","affiliation":[{"name":"Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin Ho\u0308ner","family":"zu Bentrup","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane University Health Sciences Center, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Hassett","sequence":"additional","affiliation":[{"name":"University of Cincinnati College of Medicine, Department of Molecular Genetics, Biochemistry, and Microbiology, Cincinnati, Ohio 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara H.","family":"Iglewski","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, Rochester, New York 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Sauer","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13902"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Schurr","sequence":"additional","affiliation":[{"name":"University of Colorado at Denver and Health Sciences Center, Department of Microbiology, Aurora, Colorado 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02413.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.13.3598-3604.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1111\/j.1365-2958.2005.04743.x","volume":"57","year":"2005","unstructured":"Boles, B. 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