{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:31:38Z","timestamp":1791250298707,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T00:00:00Z","timestamp":1208217600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    is a facultative intracellular pathogen responsible for severe lung disease in humans, known as legionellosis or Legionnaires' disease. Previously, we reported on the \u223c60-kDa glucosyltransferase (Lgt1) from\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    , which modified eukaryotic elongation factor 1A. In the present study, using\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    Philadelphia-1, Lens, Paris, and Corby genome databases, we identified several genes coding for proteins with considerable sequence homology to Lgt1. These new enzymes form three subfamilies, termed Lgt1 to -3, glucosylate mammalian elongation factor eEF1A at serine-53, inhibit its activity, and subsequently kill target eukaryotic cells. Expression studies on\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    grown in broth medium or in\n                    <jats:italic>Acanthamoeba castellanii<\/jats:italic>\n                    revealed that production of Lgt1 was maximal at stationary phase of broth culture or during the late phase of\n                    <jats:italic>Legionella<\/jats:italic>\n                    -host cell interaction, respectively. In contrast, synthesis of Lgt3 peaked during the lag phase of liquid culture and at early steps of bacterium-amoeba interaction. Thus, the data indicate that members of the\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    glucosyltransferase family are differentially regulated, affect protein synthesis of host cells, and represent potential virulence factors of\n                    <jats:italic>Legionella<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/jb.01798-07","type":"journal-article","created":{"date-parts":[[2008,2,15]],"date-time":"2008-02-15T21:05:25Z","timestamp":1203109525000},"page":"3026-3035","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":151,"title":["Lgt: a Family of Cytotoxic Glucosyltransferases Produced by\n                    <i>Legionella pneumophila<\/i>"],"prefix":"10.1128","volume":"190","author":[{"given":"Yury","family":"Belyi","sequence":"first","affiliation":[{"name":"Gamaleya Research Institute, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Tabakova","sequence":"additional","affiliation":[{"name":"Gamaleya Research Institute, Moscow 123098, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Stahl","sequence":"additional","affiliation":[{"name":"Institute for Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, D-79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Aktories","sequence":"additional","affiliation":[{"name":"Institute for Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, D-79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/nrmicro1150","volume":"3","year":"2005","unstructured":"Aktories, K., and J. 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