{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:01:46Z","timestamp":1791907306320,"version":"4.3.4"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T00:00:00Z","timestamp":1242345600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2009,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Several mycoplasma species feature a membrane protrusion at a cell pole, and unknown mechanisms provide gliding motility in the direction of the pole defined by the protrusion.\n            <jats:italic>Mycoplasma gallisepticum<\/jats:italic>\n            , an avian pathogen, is known to form a membrane protrusion composed of bleb and infrableb and to glide. Here, we analyzed the gliding motility of\n            <jats:italic>M. gallisepticum<\/jats:italic>\n            cells in detail. They glided in the direction of the bleb at an average speed of 0.4 \u03bcm\/s and remained attached around the bleb to a glass surface, suggesting that the gliding mechanism is similar to that of a related species,\n            <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n            . Next, to elucidate the cytoskeletal structure of\n            <jats:italic>M. gallisepticum<\/jats:italic>\n            , we stripped the envelopes by treatment with Triton X-100 under various conditions and observed the remaining structure by negative-staining transmission electron microscopy. A unique cytoskeletal structure, about 300 nm long and 100 nm wide, was found in the bleb and infrableb. The structure, resembling an asymmetrical dumbbell, is composed of five major parts from the distal end: a cap, a small oval, a rod, a large oval, and a bowl. Sonication likely divided the asymmetrical dumbbell into a core and other structures. The cytoskeletal structures of\n            <jats:italic>M. gallisepticum<\/jats:italic>\n            were compared with those of\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            in detail, and the possible protein components of these structures were considered.\n          <\/jats:p>","DOI":"10.1128\/jb.01823-08","type":"journal-article","created":{"date-parts":[[2009,3,14]],"date-time":"2009-03-14T00:49:30Z","timestamp":1236991770000},"page":"3256-3264","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Cytoskeletal Asymmetrical Dumbbell Structure of a Gliding Mycoplasma,\n            <i>Mycoplasma gallisepticum<\/i>\n            , Revealed by Negative-Staining Electron Microscopy"],"prefix":"10.1128","volume":"191","author":[{"given":"Daisuke","family":"Nakane","sequence":"first","affiliation":[{"name":"Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Miyata","sequence":"additional","affiliation":[{"name":"Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.8.2821-2828.2006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1099\/00207713-20-1-35","volume":"20","year":"1970","unstructured":"Aluotto, B. 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