{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:58:26Z","timestamp":1761919106744},"reference-count":53,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The ferric uptake regulatory protein, Fur, functions as a global regulatory protein of gene transcription in the mucosal pathogen\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            . We have shown previously that several\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            Fur-repressed genes are expressed in vivo during mucosal gonococcal infection in men, which suggests that this organism infects in an iron-limited environment and that Fur is expressed under these conditions. In this study we have demonstrated expression of the gonococcal\n            <jats:italic>fur<\/jats:italic>\n            gene in vitro, in human cervical epithelial cells, and in specimens from female subjects with uncomplicated gonococcal infection. In vitro studies confirmed that the expression of the gonococcal\n            <jats:italic>fur<\/jats:italic>\n            gene was repressed during growth under iron-replete growth conditions but that a basal level of the protein was maintained. Using GFP transcriptional fusions constructed from specific Fur binding sequences within the\n            <jats:italic>fur<\/jats:italic>\n            promoter\/operator region, we determined that this operator region was functional during\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            infection of cervical epithelial cells. Furthermore, reverse transcription-PCR analysis, as well as microarray analysis, using a custom\n            <jats:italic>Neisseria<\/jats:italic>\n            Fur and iron regulon microarray revealed that several Fur- and iron-regulated genes were expressed during\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            infection of cervical epithelial cells. Microarray analysis of specimens obtained from female subjects with uncomplicated gonococcal infection corroborated our in vitro findings and point toward a key role of gonococcal Fur- and iron-regulated genes in gonococcal disease.\n          <\/jats:p>","DOI":"10.1128\/jb.01830-07","type":"journal-article","created":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T02:36:47Z","timestamp":1204339007000},"page":"3129-3139","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Expression of the Gonococcal Global Regulatory Protein Fur and Genes Encompassing the Fur and Iron Regulon during In Vitro and In Vivo Infection in Women"],"prefix":"10.1128","volume":"190","author":[{"given":"Sarika","family":"Agarwal","sequence":"first","affiliation":[{"name":"Department of Medicine, Section of Molecular Medicine"},{"name":"Department of Medicine, Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shite","family":"Sebastian","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Molecular Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Borys","family":"Szmigielski","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Molecular Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter A.","family":"Rice","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline A.","family":"Genco","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Molecular Medicine"},{"name":"Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4281-4287.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1046\/j.1365-2958.2002.03113.x","volume":"45","year":"2002","unstructured":"Baichoo, N., T. 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