{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T01:20:38Z","timestamp":1771982438927,"version":"3.50.1"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,6,15]],"date-time":"2013-06-15T00:00:00Z","timestamp":1371254400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2013,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Nontypeable\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Haemophilus influenzae<\/jats:named-content>\n            (NTHi) is one of the leading causes of noninvasive mucosal infections, such as otitis media, sinusitis, and conjunctivitis. During its life cycle, NTHi is exposed to different CO\n            <jats:sub>2<\/jats:sub>\n            levels, which vary from \u223c0.04% in ambient air during transmission to a new host to over 5% in the respiratory tract and tissues of the human host during colonization and disease. We used the next-generation sequencing Tn-seq technology to identify genes essential for NTHi adaptation to changes in environmental CO\n            <jats:sub>2<\/jats:sub>\n            levels. It appeared that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. influenzae<\/jats:named-content>\n            carbonic anhydrase (HICA), which catalyzes the reversible hydration of CO\n            <jats:sub>2<\/jats:sub>\n            to bicarbonate, is a molecular factor that is conditionally essential for NTHi survival in ambient air. Growth of NTHi \u0394\n            <jats:italic>can<\/jats:italic>\n            strains was restored under 5% CO\n            <jats:sub>2<\/jats:sub>\n            -enriched conditions, by supplementation of the growth medium with sodium bicarbonate, or by genetic complementation with the\n            <jats:italic>can<\/jats:italic>\n            gene. Finally, we showed that HICA not only is essential for environmental survival but also appeared to be important for intracellular survival in host cells. Hence, HICA is important for NTHi niche adaptation.\n          <\/jats:p>","DOI":"10.1128\/jb.01870-12","type":"journal-article","created":{"date-parts":[[2013,4,6]],"date-time":"2013-04-06T04:37:25Z","timestamp":1365223045000},"page":"2737-2746","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Nontypeable Haemophilus influenzae Carbonic Anhydrase Is Important for Environmental and Intracellular Survival"],"prefix":"10.1128","volume":"195","author":[{"given":"Jeroen D.","family":"Langereis","sequence":"first","affiliation":[{"name":"Laboratory of Pediatric Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldert","family":"Zomer","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands"},{"name":"Centre for Molecular and Biomolecular Informatics, Nijmegen Centre for Molecular Life Sciences, Radboud University Medical Centre, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hendrik G.","family":"Stunnenberg","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University Medical Centre, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Burghout","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter W. 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