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While the intracellular lifestyle of<jats:italic>Brucella<\/jats:italic>is poorly understood and few virulence factors have been identified, components of a quorum-sensing pathway in<jats:italic>Brucella<\/jats:italic>have recently been identified. The LuxR-type regulatory protein, VjbR, and an<jats:italic>N<\/jats:italic>-acylhomoserine lactone signaling molecule are both involved in regulating expression of the<jats:italic>virB<\/jats:italic>-encoded type IV secretion system. We have identified a second LuxR-type regulatory protein (BlxR) in<jats:italic>Brucella<\/jats:italic>. Microarray analysis of a<jats:italic>blxR<\/jats:italic>mutant suggests that BlxR regulates the expression of a number of genes, including those encoding the type IV secretion system and flagella. Confirming these results, deletion of<jats:italic>blxR<\/jats:italic>in<jats:italic>B. melitensis<\/jats:italic>reduced the transcriptional activities of promoters for the<jats:italic>virB<\/jats:italic>operon, flagellar genes, and another putative virulence factor gene,<jats:italic>bopA<\/jats:italic>. Furthermore, our data suggested that both BlxR and VjbR are positively autoregulated and cross-regulate the expression of each other. The<jats:italic>blxR<\/jats:italic>deletion strain exhibited reduced growth in macrophages, similar to that observed for a<jats:italic>vjbR<\/jats:italic>deletion strain. However, unlike the<jats:italic>vjbR<\/jats:italic>deletion, the<jats:italic>blxR<\/jats:italic>deletion did not fully attenuate virulence in mice. More strikingly, bioluminescent imaging revealed that dissemination of the<jats:italic>blxR<\/jats:italic>mutant was similar to that of wild-type<jats:italic>B. melitensis<\/jats:italic>, while the<jats:italic>vjbR<\/jats:italic>mutant was defective for systemic spread in IRF-1<jats:sup>\u2212\/\u2212<\/jats:sup>mice, suggesting that these regulators are not functionally redundant but that they converge in a common pathway regulating bacterial processes.<\/jats:p>","DOI":"10.1128\/jb.01915-07","type":"journal-article","created":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T02:36:47Z","timestamp":1204339007000},"page":"3274-3282","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Putative Quorum-Sensing Regulator BlxR of<i>Brucella melitensis<\/i>Regulates Virulence Factors Including the Type IV Secretion System and Flagella"],"prefix":"10.1128","volume":"190","author":[{"given":"Amy A.","family":"Rambow-Larsen","sequence":"first","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gireesh","family":"Rajashekara","sequence":"additional","affiliation":[{"name":"Food Animal Health Research Program, Ohio Agricultural and Research Development Center, Department of Veterinary Preventive Medicine, Ohio State University, Wooster, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Splitter","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5343-5351.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4255-4263.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3702-3713.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00219-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1073\/pnas.032514299","volume":"99","year":"2002","unstructured":"Boschiroli, M. 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