{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:23:32Z","timestamp":1790439812436,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,4,15]],"date-time":"2007-04-15T00:00:00Z","timestamp":1176595200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2007,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Two types of adhesive fimbriae are expressed by\n            <jats:italic>Actinomyces<\/jats:italic>\n            ; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of\n            <jats:italic>Actinomyces naeslundii<\/jats:italic>\n            strain MG-1. By using immunoelectron microscopy and biochemical analysis, we showed that the\n            <jats:italic>fimQ<\/jats:italic>\n            -\n            <jats:italic>fimP<\/jats:italic>\n            -\n            <jats:italic>srtC1<\/jats:italic>\n            -\n            <jats:italic>fimR<\/jats:italic>\n            gene cluster encodes a fimbrial structure (designated type 1) that contains a major subunit, FimP, forming the shaft and a minor subunit, FimQ, located primarily at the tip. Similarly, the\n            <jats:italic>fimB<\/jats:italic>\n            -\n            <jats:italic>fimA<\/jats:italic>\n            -\n            <jats:italic>srtC2<\/jats:italic>\n            gene cluster encodes a distinct fimbrial structure (designated type 2) composed of a shaft protein, FimA, and a tip protein, FimB. By using allelic exchange, we constructed an in-frame deletion mutant that lacks the SrtC2 sortase. This mutant produces abundant type 1 fimbriae and expresses the monomeric FimA and FimB proteins, but it does not assemble type 2 fimbriae. Thus, SrtC2 is a fimbria-specific sortase that is essential for assembly of the type 2 fimbriae. Together, our experiments pave the way for several lines of molecular investigation that are necessary to elucidate the fimbrial assembly pathways in\n            <jats:italic>Actinomyces<\/jats:italic>\n            and their function in the pathogenesis of different biofilm-related oral diseases.\n          <\/jats:p>","DOI":"10.1128\/jb.01952-06","type":"journal-article","created":{"date-parts":[[2007,2,4]],"date-time":"2007-02-04T01:19:29Z","timestamp":1170551969000},"page":"3156-3165","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Sortase-Catalyzed Assembly of Distinct Heteromeric Fimbriae in\n            <i>Actinomyces naeslundii<\/i>"],"prefix":"10.1128","volume":"189","author":[{"given":"Arunima","family":"Mishra","sequence":"first","affiliation":[{"name":"Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, Connecticut 06030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asis","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, Connecticut 06030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John O.","family":"Cisar","sequence":"additional","affiliation":[{"name":"Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung","family":"Ton-That","sequence":"additional","affiliation":[{"name":"Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, Connecticut 06030"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1099\/00221287-137-8-1971","volume":"137","year":"1991","unstructured":"Cisar, J. 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