{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T15:38:46Z","timestamp":1773070726518,"version":"3.50.1"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Bacteriol."],"published-print":{"date-parts":[[2015,1,15]]},"abstract":"<jats:p>The Gram-negative commensal bacterium nontypeable<jats:named-content content-type=\"genus-species\">Haemophilus influenzae<\/jats:named-content>(NTHI) can cause respiratory tract diseases that include otitis media, sinusitis, exacerbations of chronic obstructive pulmonary disease, and bronchitis. During colonization and infection, NTHI withstands oxidative stress generated by reactive oxygen species produced endogenously, by the host, and by other copathogens and flora. These reactive oxygen species include superoxide, hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>), and hydroxyl radicals, whose killing is amplified by iron via the Fenton reaction. We previously identified genes that encode proteins with putative roles in protection of the NTHI isolate strain 86-028NP against oxidative stress. These include catalase (HktE), peroxiredoxin\/glutaredoxin (PgdX), and a ferritin-like protein (Dps). Strains were generated with mutations in<jats:italic>hktE<\/jats:italic>,<jats:italic>pgdX<\/jats:italic>, and<jats:italic>dps<\/jats:italic>. The<jats:italic>hktE<\/jats:italic>mutant and a<jats:italic>pgdX hktE<\/jats:italic>double mutant were more sensitive than the parent to killing by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Conversely, the<jats:italic>pgdX<\/jats:italic>mutant was more resistant to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>due to increased catalase activity. Supporting the role of killing via the Fenton reaction, binding of iron by Dps significantly mitigated the effect of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-mediated killing. NTHI thus utilizes several effectors to resist oxidative stress, and regulation of free iron is critical to this protection. These mechanisms will be important for successful colonization and infection by this opportunistic human pathogen.<\/jats:p>","DOI":"10.1128\/jb.01973-14","type":"journal-article","created":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T06:47:03Z","timestamp":1415083623000},"page":"277-285","source":"Crossref","is-referenced-by-count":16,"title":["Overlapping and Complementary Oxidative Stress Defense Mechanisms in Nontypeable Haemophilus influenzae"],"prefix":"10.1128","volume":"197","author":[{"given":"Alistair","family":"Harrison","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth D.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Robert 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