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While<jats:italic>recA<\/jats:italic>mutants in most bacterial species are hypersensitive to UV damage, surprisingly a<jats:italic>B. abortus recA<\/jats:italic>null mutant conferred only modest sensitivity. We considered the presence of a second RecA protein to account for this modest UV sensitivity. Analyses of the<jats:italic>Brucella<\/jats:italic>spp. genomes and our molecular studies documented the presence of only one<jats:italic>recA<\/jats:italic>gene, suggesting a RecA-independent repair process. Searches of the available<jats:italic>Brucella<\/jats:italic>genomes revealed some homology between RecA and RadA, a protein implicated in<jats:italic>E. coli<\/jats:italic>DNA repair. We considered the possibility that<jats:italic>B. abortus<\/jats:italic>RadA might be compensating for the loss of RecA by promoting similar repair activities. We present functional analyses that demonstrated that<jats:italic>B. abortus<\/jats:italic>RadA complements a<jats:italic>radA<\/jats:italic>defect in<jats:italic>E. coli<\/jats:italic>but could not act in place of the<jats:italic>B. abortus<\/jats:italic>RecA. We show that RecA but not RadA was required for survival in macrophages. We also discovered that<jats:italic>recA<\/jats:italic>was expressed at high constitutive levels, due to constitutive LexA cleavage by RecA, with little induction following DNA damage. Higher basal levels of RecA and its SOS-regulated gene products might protect against DNA damage experienced following the oxidative burst within macrophages.<\/jats:p>","DOI":"10.1128\/jb.01994-05","type":"journal-article","created":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T23:17:58Z","timestamp":1151709478000},"page":"5187-5195","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["RecA and RadA Proteins of<i>Brucella abortus<\/i>Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst"],"prefix":"10.1128","volume":"188","author":[{"given":"Christelle M.","family":"Roux","sequence":"first","affiliation":[{"name":"Department of Biology, University of Louisiana, Lafayette, Louisiana 70504"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natha J.","family":"Booth","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Louisiana, Lafayette, Louisiana 70504"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan H.","family":"Bellaire","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventative Medicine, Iowa State University, Ames, Iowa 50011"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason M.","family":"Gee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"R. 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