{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:47:18Z","timestamp":1791103638756,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio cholerae<\/jats:named-content>\n            , a noninvasive bacterium, colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes the severe fluid loss characteristic of the disease cholera. In this study, we demonstrate that adherence of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            to the intestinal epithelial cell line INT 407 strongly induces the expression of the major virulence genes\n            <jats:italic>ctxAB<\/jats:italic>\n            and\n            <jats:italic>tcpA<\/jats:italic>\n            and the virulence regulatory gene\n            <jats:italic>toxT<\/jats:italic>\n            . No induction of\n            <jats:italic>toxR<\/jats:italic>\n            and\n            <jats:italic>tcpP<\/jats:italic>\n            , which encode transcriptional activators of\n            <jats:italic>toxT<\/jats:italic>\n            , was observed in adhered bacteria, and the adherence-dependent upregulation of\n            <jats:italic>toxT<\/jats:italic>\n            expression was independent of ToxR and TcpP. A sharp increase in the expression of the\n            <jats:italic>vieA<\/jats:italic>\n            gene, which encodes a cyclic di-GMP (c-di-GMP) phosphodiesterase, was observed in INT 407-adhered\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            immediately after infection. Induction of\n            <jats:italic>toxT<\/jats:italic>\n            ,\n            <jats:italic>ctxAB<\/jats:italic>\n            , and\n            <jats:italic>tcpA<\/jats:italic>\n            in INT 407-adhered\n            <jats:italic>vieA<\/jats:italic>\n            mutant strain O395 \u0394\n            <jats:italic>vieA<\/jats:italic>\n            was consistently lower than in the parent strain, although no effect was observed in unadhered bacteria, suggesting that VieA has a role in the upregulation of\n            <jats:italic>toxT<\/jats:italic>\n            expression specifically in host cell-adhered\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            . Furthermore, though VieA has both a DNA binding helix-turn-helix domain and an EAL domain conferring c-di-GMP phosphodiesterase activity, the c-di-GMP phosphodiesterase activity of VieA is necessary and sufficient for the upregulation of\n            <jats:italic>toxT<\/jats:italic>\n            expression.\n          <\/jats:p>","DOI":"10.1128\/jb.02127-12","type":"journal-article","created":{"date-parts":[[2013,2,23]],"date-time":"2013-02-23T11:58:53Z","timestamp":1361620733000},"page":"2004-2010","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Host Cell Contact Induces Expression of Virulence Factors and VieA, a Cyclic di-GMP Phosphodiesterase, in Vibrio cholerae"],"prefix":"10.1128","volume":"195","author":[{"given":"Amit K.","family":"Dey","sequence":"first","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abha","family":"Bhagat","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rukhsana","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15328-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.9.2833"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1084\/jem.168.4.1487","article-title":"Toxin, toxin co-regulated pili and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans","volume":"168","author":"Herrington DA","year":"1988","unstructured":"HerringtonDA HallRH LosonskyG MekalanosJJ TaylorRK LevineMM. 1988. 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