{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T17:18:54Z","timestamp":1772039934312,"version":"3.50.1"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,4,15]],"date-time":"2013-04-15T00:00:00Z","timestamp":1365984000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2013,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycoplasma pneumoniae<\/jats:named-content>\n            exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            , random transposon mutagenesis was employed to generate mutants with gliding-deficient phenotypes. Transposon insertions in the only annotated Ser\/Thr protein kinase gene (\n            <jats:italic>prkC<\/jats:italic>\n            ; MPN248) and its cognate phosphatase gene (\n            <jats:italic>prpC<\/jats:italic>\n            ; MPN247) in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            resulted in significant and contrasting effects on gliding frequencies.\n            <jats:italic>prkC<\/jats:italic>\n            mutant cells glided at approximately half the frequency of wild-type cells, while\n            <jats:italic>prpC<\/jats:italic>\n            mutant cells glided more than twice as frequently as wild-type cells. Phosphoprotein staining confirmed the association between phosphorylation of the cytoskeletal proteins HMW1 and HMW2 and membrane protein P1 and the gliding phenotype. When the\n            <jats:italic>prpC<\/jats:italic>\n            mutant was complemented by transposon delivery of a wild-type copy of the\n            <jats:italic>prpC<\/jats:italic>\n            allele, gliding frequencies and phosphorylation levels returned to the wild-type standard. Surprisingly, delivery of the recombinant wild-type\n            <jats:italic>prkC<\/jats:italic>\n            allele dramatically increased gliding frequency to a level approximately 3-fold greater than that of wild-type in the\n            <jats:italic>prkC<\/jats:italic>\n            mutant. Collectively, these data suggest that PrkC and PrpC work in opposition in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. pneumoniae<\/jats:named-content>\n            to influence gliding frequency.\n          <\/jats:p>","DOI":"10.1128\/jb.02277-12","type":"journal-article","created":{"date-parts":[[2013,2,9]],"date-time":"2013-02-09T03:55:40Z","timestamp":1360382140000},"page":"1750-1757","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Protein Kinase\/Phosphatase Function Correlates with Gliding Motility in Mycoplasma pneumoniae"],"prefix":"10.1128","volume":"195","author":[{"given":"Clinton A.","family":"Page","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duncan C.","family":"Krause","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.697-728.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"3278","DOI":"10.1093\/nar\/28.17.3278","article-title":"Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames","volume":"28","author":"Dandekar T","year":"2000","unstructured":"DandekarT HuynenM RegulaJT UeberleB ZimmermannCU AndradeMA DoerksT Sanchez-PulidoL SnelB SuyamaM YuanYP HerrmannR BorkP. 2000. 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