{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:11:59Z","timestamp":1791868319884,"version":"4.3.4"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Bacteriol."],"published-print":{"date-parts":[[2015,1,15]]},"abstract":"<jats:p>Proper expression of the<jats:italic>dev<\/jats:italic>operon is important for normal development of<jats:named-content content-type=\"genus-species\">Myxococcus xanthus<\/jats:named-content>. When starved, these bacteria coordinate their gliding movements to build mounds that become fruiting bodies as some cells differentiate into spores. Mutations in the<jats:italic>devTRS<\/jats:italic>genes impair sporulation. Expression of the operon occurs within nascent fruiting bodies and depends in part on C signaling. Here, we report that expression of the<jats:italic>dev<\/jats:italic>operon, like that of several other C-signal-dependent genes, is subject to combinatorial control by the transcription factors MrpC2 and FruA. A DNA fragment upstream of the<jats:italic>dev<\/jats:italic>promoter was bound by a protein in an extract containing MrpC2, protecting the region spanning positions \u221277 to \u221254. Mutations in this region impaired binding of purified MrpC2 and abolished developmental expression of reporter fusions. The association of MrpC2 and\/or its longer form, MrpC, with the<jats:italic>dev<\/jats:italic>promoter region depended on FruA<jats:italic>in vivo<\/jats:italic>, based on chromatin immunoprecipitation analysis, and purified FruA appeared to bind cooperatively with MrpC2 to DNA just upstream of the<jats:italic>dev<\/jats:italic>promoter<jats:italic>in vitro<\/jats:italic>. We conclude that cooperative binding of the two proteins to this promoter-proximal site is crucial for<jats:italic>dev<\/jats:italic>expression. 5\u2032 deletion analysis implied a second upstream positive regulatory site, which corresponded to a site of weak cooperative binding of MrpC2 and FruA and boosted<jats:italic>dev<\/jats:italic>expression 24 h into development. This site is unique among the C-signal-dependent genes studied so far. Deletion of this site in the<jats:named-content content-type=\"genus-species\">M. xanthus<\/jats:named-content>chromosome did not impair sporulation under laboratory conditions.<\/jats:p>","DOI":"10.1128\/jb.02310-14","type":"journal-article","created":{"date-parts":[[2014,10,28]],"date-time":"2014-10-28T03:10:27Z","timestamp":1414465827000},"page":"240-251","source":"Crossref","is-referenced-by-count":24,"title":["Combinatorial Regulation of the<i>dev<\/i>Operon by MrpC2 and FruA during Myxococcus xanthus Development"],"prefix":"10.1128","volume":"197","author":[{"given":"Ashleigh","family":"Campbell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poorna","family":"Viswanathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bongjun","family":"Son","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shreya","family":"Saha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Kroos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2014,10,27]]},"reference":[{"key":"key20180919081431_B1","volume-title":"Myxobacteria: genomics, cellular and molecular 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