{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:08:29Z","timestamp":1791598109891,"version":"4.3.4"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Bacteriol."],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The<jats:italic>blp<\/jats:italic>locus of<jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>secretes and regulates bacteriocins, which mediate both intra- and interspecific competition in the human nasopharynx. There are four major alleles of the gene<jats:italic>blpH<\/jats:italic>, which encodes the receptor responsible for activating the<jats:italic>blp<\/jats:italic>locus when bound to one of four distinct peptide pheromones (BlpC). The allelic variation of<jats:italic>blpH<\/jats:italic>is presumably explained by a need to restrict cross talk between competing strains. The BlpH protein sequences have polymorphisms distributed throughout the sequence, making identification of the peptide binding site difficult to predict. To identify the pheromone binding sites that dictate pheromone specificity, we have characterized the four major variants and two naturally occurring chimeric versions of<jats:italic>blpH<\/jats:italic>in which recombination events appear to have joined two distinct<jats:italic>blpH<\/jats:italic>alleles together. Using these allelic variants, a series of laboratory-generated chimeric<jats:italic>blpH<\/jats:italic>alleles, and site-directed mutants of both the receptor and peptide, we have demonstrated that BlpC binding to some BlpH types involves an electrostatic interaction between the oppositely charged residues of BlpC and the first transmembrane domain of BlpH. An additional recognition site was identified in the second extracellular loop. We identified naturally occurring BlpH types that have the capacity to respond to more than one BlpC type; however, this change in specificity results in a commensurate drop in overall sensitivity. These natural recombination events were presumably selected for to balance the need to sense bacteriocin-secreting neighbors with the need to turn on bacteriocin production at a low density.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>Bacteria use quorum sensing to optimize gene expression to accommodate for local bacterial density and diffusion rates. To prevent interception of quorum-sensing signals by neighboring strains, the genomes of single species often encode strain-specific signal\/receptor pairs. The<jats:italic>blp<\/jats:italic>locus in<jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>that drives bacteriocin secretion is controlled by quorum sensing that involves the interaction of the signal\/receptor pair BlpC\/BlpH. We show that the pneumococcal population can be divided into several distinct BlpC\/BlpH pairs; however, there are examples of naturally occurring chimeric receptors that can bind to more than one BlpC type. The trade-off for this broadened specificity is a loss of overall receptor sensitivity. This suggests that under certain conditions, the advantage of signal interception can trump the requirements for self-induction.<\/jats:p>","DOI":"10.1128\/jb.02432-14","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T02:33:48Z","timestamp":1422326028000},"page":"1236-1248","source":"Crossref","is-referenced-by-count":28,"title":["An Electrostatic Interaction between BlpC and BlpH Dictates Pheromone Specificity in the Control of Bacteriocin Production and Immunity in Streptococcus pneumoniae"],"prefix":"10.1128","volume":"197","author":[{"given":"Marisa D.","family":"Pinchas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan C.","family":"LaCross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Dawid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2015,1,26]]},"reference":[{"key":"key20180919081448_B1","first-page":"692","article-title":"Epidemiologic studies of Streptococcus pneumoniae in infants. 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