{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T04:12:15Z","timestamp":1777176735931,"version":"3.51.4"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            MntH is the only high-affinity manganese transporter identified in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            . A previous study showed that MntH is required for the wild-type virulence of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella abortus<\/jats:named-content>\n            2308 in mice (Anderson ES, et al., Infect. Immun.\n            <jats:bold>77:<\/jats:bold>\n            3466\u20133474, 2009) and indicated that the\n            <jats:italic>mntH<\/jats:italic>\n            gene is regulated in a manganese-responsive manner in this strain by a Mur homolog. In the study presented here, the transcriptional start site for\n            <jats:italic>mntH<\/jats:italic>\n            in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            2308 was determined by primer extension analysis. Specific interactions between Mur and the\n            <jats:italic>mntH<\/jats:italic>\n            promoter region were demonstrated in an electrophoretic mobility shift assay (EMSA), and a Mur binding site was identified in the \u221255 to \u221224 region of the\n            <jats:italic>mntH<\/jats:italic>\n            promoter by DNase I footprint analysis. The specificity of the interaction of Mur with the putative Mur box was further evaluated by EMSA employing oligonucleotides in which the consensus nucleotides in this region were substituted. These studies not only confirm a direct role for Mur in the Mn-responsive regulation of\n            <jats:italic>mntH<\/jats:italic>\n            expression in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella abortus<\/jats:named-content>\n            2308 but also identify the\n            <jats:italic>cis<\/jats:italic>\n            -acting elements upstream of\n            <jats:italic>mntH<\/jats:italic>\n            that are responsible for this regulation.\n          <\/jats:p>","DOI":"10.1128\/jb.05296-11","type":"journal-article","created":{"date-parts":[[2011,11,19]],"date-time":"2011-11-19T04:14:33Z","timestamp":1321676073000},"page":"561-566","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Mur Regulates the Gene Encoding the Manganese Transporter MntH in Brucella abortus 2308"],"prefix":"10.1128","volume":"194","author":[{"given":"Evan A.","family":"Menscher","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clayton C.","family":"Caswell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric S.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"R. 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