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In its coevolution with humans, the streamlining of the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            genome has resulted in a significant reduction in metabolic pathways, one being purine nucleotide biosynthesis. Bioinformatic analysis has revealed that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            lacks the enzymatic machinery for\n            <jats:italic>de novo<\/jats:italic>\n            production of IMP, the first purine nucleotide formed during GTP and ATP biosynthesis. This suggests that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            must rely heavily on salvage of purines from the environment. In this study, we deleted several genes putatively involved in purine salvage and processing. The growth and survival of these mutants were analyzed in both nutrient-rich and minimal media, and the results confirmed the presence of a robust purine salvage pathway in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            . Of the two phosphoribosyltransferase genes found in the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            genome, only\n            <jats:italic>gpt<\/jats:italic>\n            appears to be essential, and an \u0394\n            <jats:italic>apt<\/jats:italic>\n            mutant strain was still capable of growth on adenine, suggesting that adenine processing via Apt is not essential. Deletion of the putative nucleoside phosphorylase gene\n            <jats:italic>deoD<\/jats:italic>\n            resulted in an inability of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            to grow on purine nucleosides or the purine base adenine. Our results suggest a purine requirement for growth of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            in standard media, indicating that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            possesses the ability to utilize purines and nucleosides from the environment in the absence of a\n            <jats:italic>de novo<\/jats:italic>\n            purine nucleotide biosynthesis pathway.\n          <\/jats:p>","DOI":"10.1128\/jb.05757-11","type":"journal-article","created":{"date-parts":[[2011,12,23]],"date-time":"2011-12-23T05:12:30Z","timestamp":1324617150000},"page":"839-854","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":80,"title":["Helicobacter pylori Relies Primarily on the Purine Salvage Pathway for Purine Nucleotide Biosynthesis"],"prefix":"10.1128","volume":"194","author":[{"given":"George","family":"Liechti","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna B.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1007\/s001099900067","article-title":"Analysis of the genetic diversity of Helicobacter pylori: the tale of two genomes","volume":"77","author":"Alm RA","year":"1999","unstructured":"AlmRA TrustTJ. 1999. 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