{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:53:42Z","timestamp":1792205622819,"version":"4.4.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2011,11,15]],"date-time":"2011-11-15T00:00:00Z","timestamp":1321315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2011,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacterial type IV secretion systems (T4SSs) are involved in processes such as bacterial conjugation and protein translocation to animal cells. In this work, we have switched the substrates of T4SSs involved in pathogenicity for DNA transfer. Plasmids containing part of the conjugative machinery of plasmid R388 were transferred by the T4SS of human facultative intracellular pathogen\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Bartonella henselae<\/jats:named-content>\n            to both recipient bacteria and human vascular endothelial cells. About 2% of the human cells expressed a green fluorescent protein (GFP) gene from the plasmid. Plasmids of different sizes were transferred with similar efficiencies.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. henselae<\/jats:named-content>\n            codes for two T4SSs: VirB\/VirD4 and Trw. A \u0394\n            <jats:italic>virB<\/jats:italic>\n            mutant strain was transfer deficient, while a \u0394\n            <jats:italic>trwE<\/jats:italic>\n            mutant was only slightly impaired in DNA transfer. DNA transfer was in all cases dependent on protein TrwC of R388, the conjugative relaxase, implying that it occurs by a conjugation-like mechanism. A DNA helicase-deficient mutant of TrwC could not promote DNA transfer. In the absence of TrwB, the coupling protein of R388, DNA transfer efficiency dropped 1 log. The same low efficiency was obtained with a TrwB point mutation in the region involved in interaction with the T4SS. TrwB interacted with VirB10 in a bacterial two-hybrid assay, suggesting that it may act as the recruiter of the R388 substrate for the VirB\/VirD4 T4SS. A TrwB ATPase mutant behaved as dominant negative, dropping DNA transfer efficiency to almost null levels.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. henselae<\/jats:named-content>\n            bacteria recovered from infected human cells could transfer the mobilizable plasmid into recipient\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            under certain conditions, underscoring the versatility of T4SSs.\n          <\/jats:p>","DOI":"10.1128\/jb.05905-11","type":"journal-article","created":{"date-parts":[[2011,9,10]],"date-time":"2011-09-10T02:50:20Z","timestamp":1315623020000},"page":"6257-6265","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Transfer of R388 Derivatives by a Pathogenesis-Associated Type IV Secretion System into both Bacteria and Human Cells"],"prefix":"10.1128","volume":"193","author":[{"given":"Esther","family":"Fern\u00e1ndez-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular, Universidad de Cantabria, and Instituto de Biomedicina y Biotecnolog\u00eda de Cantabria (IBBTEC), UC-SODERCAN-CSIC, C. 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