{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:27Z","timestamp":1791942387590,"version":"4.3.4"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2012,4,15]],"date-time":"2012-04-15T00:00:00Z","timestamp":1334448000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2012,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The brucellae are Gram-negative pathogens that cause brucellosis, a zoonosis of worldwide importance. The genus\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            includes smooth and rough species that differ in that they carry smooth and rough lipopolysaccharides, respectively.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella abortus<\/jats:named-content>\n            ,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>\n            , and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. suis<\/jats:named-content>\n            are typical smooth species. However, these smooth brucellae dissociate into rough mutants devoid of the lipopolysaccharide O-polysaccharide, a major antigen and a virulence determinant encoded in regions\n            <jats:italic>wbo<\/jats:italic>\n            (included in genomic island-2) and\n            <jats:italic>wbk<\/jats:italic>\n            . We demonstrate here the occurrence of spontaneous recombination events in those three\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            species leading to the deletion of a 5.5-kb fragment carrying the\n            <jats:italic>wbkA<\/jats:italic>\n            glycosyltranferase gene and to the appearance of rough mutants. Analysis of the recombination intermediates suggested homologous recombination between the IS\n            <jats:italic>Bm1<\/jats:italic>\n            insertion sequences flanking\n            <jats:italic>wbkA<\/jats:italic>\n            as the mechanism generating the deletion. Excision of\n            <jats:italic>wbkA<\/jats:italic>\n            was reduced but not abrogated in a\n            <jats:italic>recA<\/jats:italic>\n            -deficient mutant, showing the existence of both RecA-dependent and -independent processes. Although the involvement of the IS\n            <jats:italic>Bm1<\/jats:italic>\n            copies flanking\n            <jats:italic>wbkA<\/jats:italic>\n            suggested a transpositional event, the predicted transpositional joint could not be detected. This absence of detectable transposition was consistent with the presence of polymorphism in the inverted repeats of one of the IS\n            <jats:italic>Bm1<\/jats:italic>\n            copies. The spontaneous excision of\n            <jats:italic>wbkA<\/jats:italic>\n            represents a novel dissociation mechanism of smooth brucellae that adds to the previously described excision of genomic island-2. This IS\n            <jats:italic>Bm1<\/jats:italic>\n            -mediated\n            <jats:italic>wbkA<\/jats:italic>\n            excision and the different %GC levels of the excised fragment and of other\n            <jats:italic>wbk<\/jats:italic>\n            genes suggest that the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            <jats:italic>wbk<\/jats:italic>\n            locus is the result of at least two horizontal acquisition events.\n          <\/jats:p>","DOI":"10.1128\/jb.06561-11","type":"journal-article","created":{"date-parts":[[2012,2,11]],"date-time":"2012-02-11T05:30:54Z","timestamp":1328938254000},"page":"1860-1867","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Spontaneous Excision of the O-Polysaccharide\n            <i>wbkA<\/i>\n            Glycosyltranferase Gene Is a Cause of Dissociation of Smooth to Rough Brucella Colonies"],"prefix":"10.1128","volume":"194","author":[{"given":"Marcos","family":"Mancilla","sequence":"first","affiliation":[{"name":"Instituto de Salud Tropical y Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara M.","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria, Unidad de Sanidad Animal, Gobierno de Arag\u00f3n, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Blasco","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria, Unidad de Sanidad Animal, Gobierno de Arag\u00f3n, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Mar\u00eda","family":"Z\u00e1rraga","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"L\u00f3pez-Go\u00f1i","sequence":"additional","affiliation":[{"name":"Instituto de Salud Tropical y Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Moriy\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Salud Tropical y Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Universidad de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"4913","DOI":"10.1016\/j.vaccine.2008.07.030","article-title":"Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections","volume":"26","author":"Adone R","year":"2008","unstructured":"AdoneR FranciaM CiuchiniF. 2008. 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