{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:16:29Z","timestamp":1778915789719,"version":"3.51.4"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T00:00:00Z","timestamp":1350259200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2012,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Containment strategies for outbreaks of invasive\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Neisseria meningitidis<\/jats:named-content>\n            disease are informed by serogroup assays that characterize the polysaccharide capsule. We sought to uncover the genomic basis of conflicting serogroup assay results for an isolate (M16917) from a patient with acute meningococcal disease. To this end, we characterized the complete genome sequence of the M16917 isolate and performed a variety of comparative sequence analyses against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>\n            reference genome sequences of known serogroups. Multilocus sequence typing and whole-genome sequence comparison revealed that M16917 is a member of the ST-11 sequence group, which is most often associated with serogroup C. However, sequence similarity comparisons and phylogenetic analysis showed that the serogroup diagnostic capsule polymerase gene (\n            <jats:italic>synD<\/jats:italic>\n            ) of M16917 belongs to serogroup B. These results suggest that a capsule-switching event occurred based on homologous recombination at or around the capsule locus of M16917. Detailed analysis of this locus uncovered the locations of recombination breakpoints in the M16917 genome sequence, which led to the introduction of an \u223c2-kb serogroup B sequence cassette into the serogroup C genomic background. Since there is no currently available vaccine for serogroup B strains of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">N. meningitidis<\/jats:named-content>\n            , this kind capsule-switching event could have public health relevance as a vaccine escape mutant.\n          <\/jats:p>","DOI":"10.1128\/jb.06604-11","type":"journal-article","created":{"date-parts":[[2012,8,18]],"date-time":"2012-08-18T04:56:31Z","timestamp":1345265791000},"page":"5649-5656","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Genomic Basis of a Polyagglutinating Isolate of Neisseria meningitidis"],"prefix":"10.1128","volume":"194","author":[{"given":"Lavanya","family":"Rishishwar","sequence":"first","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee S.","family":"Katz","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nitya V.","family":"Sharma","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori","family":"Rowe","sequence":"additional","affiliation":[{"name":"Biotechnology Core Facility Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Frace","sequence":"additional","affiliation":[{"name":"Biotechnology Core Facility Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Dolan Thomas","sequence":"additional","affiliation":[{"name":"Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian H.","family":"Harcourt","sequence":"additional","affiliation":[{"name":"Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard W.","family":"Mayer","sequence":"additional","affiliation":[{"name":"Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. King","family":"Jordan","sequence":"additional","affiliation":[{"name":"School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00181-09"},{"key":"e_1_3_2_4_2","first-page":"1","article-title":"Prevention and control of meningococcal disease. Recommendations of the Advisory Committee on Immunization Practices (ACIP)","volume":"54","author":"Bilukha OO","year":"2005","unstructured":"BilukhaOO RosensteinN . 2005. Prevention and control of meningococcal disease. Recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Recommend. Rep. 54(RR-7): 1\u201321.","journal-title":"MMWR Recommend. Rep."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1111\/j.1365-2958.2008.06580.x","article-title":"Amino acid 310 determines the donor substrate specificity of serogroup W-135 and Y capsule polymerases of Neisseria meningitidis","volume":"71","author":"Claus H","year":"2009","unstructured":"ClausH StummeyerK BatzillaJ M\u00fchlenhoffM VogelU . 2009. Amino acid 310 determines the donor substrate specificity of serogroup W-135 and Y capsule polymerases of Neisseria meningitidis. Mol. Microbiol. 71: 960\u2013971.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1086\/374740","article-title":"Genetic basis for nongroupable Neisseria meningitidis","volume":"187","author":"Dolan-Livengood JM","year":"2003","unstructured":"Dolan-LivengoodJM MillerYK MartinLE UrwinR StephensDS . 2003. Genetic basis for nongroupable Neisseria meningitidis. J. Infect. Dis. 187: 1616\u20131628.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh458"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/3527608508.ch8","volume-title":"Handbook of meningococcal disease: infection biology, vaccination, clinical management","author":"Frosch M","year":"2006","unstructured":"FroschM VogelU . 2006. Structure and genetics of the meningococcal capsule, p 145\u2013162. In FroschM MaidenMCJ (ed), Handbook of meningococcal disease: infection biology, vaccination, clinical management. Wiley-VCH Verlag GmbH & Co., Weinheim, Germany."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1073\/pnas.86.5.1669","article-title":"Molecular characterization and expression in Escherichia coli of the gene complex encoding the polysaccharide capsule of Neisseria meningitidis group B","volume":"86","author":"Frosch M","year":"1989","unstructured":"FroschM WeisgerberC MeyerTF . 1989. Molecular characterization and expression in Escherichia coli of the gene complex encoding the polysaccharide capsule of Neisseria meningitidis group B. Proc. Natl. Acad. Sci. U. S. A. 86: 1669\u20131673.","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"W181","DOI":"10.1093\/nar\/gkn179","article-title":"The CGView server: a comparative genomics tool for circular genomes","volume":"36","author":"Grant JR","year":"2008","unstructured":"GrantJR StothardP . 2008. The CGView server: a comparative genomics tool for circular genomes. Nucleic Acids Res. 36: W181\u2013W184.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1086\/651505","article-title":"Population structure and capsular switching of invasive Neisseria meningitidis isolates in the pre-meningococcal conjugate vaccine era\u2014United States, 2000-2005","volume":"201","author":"Harrison LH","year":"2010","unstructured":"HarrisonLH . 2010. Population structure and capsular switching of invasive Neisseria meningitidis isolates in the pre-meningococcal conjugate vaccine era\u2014United States, 2000-2005. J. Infect. Dis. 201: 1208\u20131224.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.04.063"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026159"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-86"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msi041"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01198-10"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq284"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-2-r12"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3140"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03959"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.11.1046"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.320-328.2004"},{"key":"e_1_3_2_24_2","unstructured":"PopovicT\nAjelloGW\n. \n2003. \nNeisseria meningitidis: confirmatory identification and antimicrobial susceptibility testing. \nWorld Health Organization Geneva Switzerland."},{"key":"e_1_3_2_25_2","unstructured":"PopovicT\nAjelloGW\nFacklamR\n. \n1999. \nLaboratory manual for the diagnosis of meningitis caused by Neisseria meningitidis Streptococcus pneumoniae and Haemophilus influenzae. \nWorld Health Organization Geneva Switzerland."},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1056\/NEJM200105033441807","article-title":"Meningococcal disease","volume":"344","author":"Rosenstein NE","year":"2001","unstructured":"RosensteinNE PerkinsBA StephensDS PopovicT HughesJM . 2001. Meningococcal disease. N. Engl. J. Med. 344: 1378\u20131388.","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00182389"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"15349","DOI":"10.1074\/jbc.M208837200","article-title":"Functional relationships of the sialyltransferases involved in expression of the polysialic acid capsules of Escherichia coli K1 and K92 and Neisseria meningitidis groups B or C","volume":"278","author":"Steenbergen SM","year":"2003","unstructured":"SteenbergenSM VimrER . 2003. Functional relationships of the sialyltransferases involved in expression of the polysialic acid capsules of Escherichia coli K1 and K92 and Neisseria meningitidis groups B or C. J. Biol. Chem. 278: 15349\u201315359.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.6.1533-1539.1998"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1073\/pnas.94.1.271","article-title":"Capsule switching of Neisseria meningitidis","volume":"94","author":"Swartley JS","year":"1997","unstructured":"SwartleyJS . 1997. Capsule switching of Neisseria meningitidis. Proc. Natl. Acad. Sci. U. S. A. 94: 271\u2013276.","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1155\/2005\/216369","article-title":"Potential capsule switching from serogroup Y to B: the characterization of three such Neisseria meningitidis isolates causing invasive meningococcal disease in Canada","volume":"16","author":"Tsang RS","year":"2005","unstructured":"TsangRS . 2005. Potential capsule switching from serogroup Y to B: the characterization of three such Neisseria meningitidis isolates causing invasive meningococcal disease in Canada. Can. J. Infect. Dis. Med. Microbiol. 16: 171\u2013174.","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1056\/NEJM200001203420319","article-title":"Rapid serogroup switching in Neisseria meningitidis","volume":"342","author":"Vogel U","year":"2000","unstructured":"VogelU ClausH FroschM . 2000. Rapid serogroup switching in Neisseria meningitidis. N. Engl. J. Med. 342: 219\u2013220.","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00363-10"},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.ijmm.2006.05.004","article-title":"Genetic mechanisms for loss of encapsulation in polysialyltransferase-gene-positive meningococci isolated from healthy carriers","volume":"296","author":"Weber MV","year":"2006","unstructured":"WeberMV ClausH MaidenMC FroschM VogelU . 2006. Genetic mechanisms for loss of encapsulation in polysialyltransferase-gene-positive meningococci isolated from healthy carriers. Int. J. Med. Microbiol. 296: 475\u2013484.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5146-5153.2004"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.06604-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.06604-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:28:43Z","timestamp":1627579723000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.06604-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,15]]},"references-count":35,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2012,10,15]]}},"alternative-id":["10.1128\/JB.06604-11"],"URL":"https:\/\/doi.org\/10.1128\/jb.06604-11","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,15]]},"assertion":[{"value":"2011-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-09-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}