{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:51:27Z","timestamp":1766267487166},"reference-count":20,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1971,1,1]],"date-time":"1971-01-01T00:00:00Z","timestamp":31536000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1971,1]]},"abstract":"<jats:p>\n            A stable, non-nitrogen-fixing mutant of\n            <jats:italic>Clostridium pasteurianum<\/jats:italic>\n            W5 was isolated. The mutant has the same growth rate as the wild type on ammonia nitrogen, but is not able to grow on N\n            <jats:sub>2<\/jats:sub>\n            . Cell-free extracts of the derepressed mutant are unable to fix nitrogen, or reduce the alternate substrates of nitrogenase such as cyanide, azide, or acetylene. Recombination with wild-type components from a diethylaminoethyl cellulose column indicates that the mutant contains component II, but is lacking component I activity.\n          <\/jats:p>","DOI":"10.1128\/jb.105.1.65-69.1971","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T09:28:42Z","timestamp":1578043722000},"page":"65-69","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Mutant of\n            <i>Clostridium pasteurianum<\/i>\n            That Does Not Fix Nitrogen"],"prefix":"10.1128","volume":"105","author":[{"given":"Meredith Ann","family":"Simon","sequence":"first","affiliation":[{"name":"Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Winston J.","family":"Brill","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1073\/pnas.53.3.532","article-title":"Nitrogen fixation: hydrosulfite as electron donor with cell-free preparations of Azotobacter vinelandti and Rhodosptrillum rubrum","volume":"53","author":"Bulen W. 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