{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T19:50:59Z","timestamp":1771530659675,"version":"3.50.1"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1972,2,1]],"date-time":"1972-02-01T00:00:00Z","timestamp":65750400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1972,2]]},"abstract":"<jats:p>\n            The properties of a specific system for the transport of\n            <jats:italic>S<\/jats:italic>\n            -adenosylmethionine in yeast are described. The process was\n            <jats:italic>p<\/jats:italic>\n            H-, temperature-, and energy-dependent, and showed saturation kinetics. The\n            <jats:italic>K<\/jats:italic>\n            <jats:sub>m<\/jats:sub>\n            for the system was 3.3 \u00d7 10\n            <jats:sup>\u22126<\/jats:sup>\n            <jats:sc>m<\/jats:sc>\n            . Of the\n            <jats:italic>S<\/jats:italic>\n            -adenosylmethionine moieties tested, only\n            <jats:italic>S<\/jats:italic>\n            -adenosylhomocysteine competitively inhibited the uptake of the adenosylsulfonium compound. Adenine, adenosine, methionine, homocysteine, and the sulfonium compound\n            <jats:italic>S<\/jats:italic>\n            -methylmethionine were without effect. The analogue\n            <jats:italic>S<\/jats:italic>\n            -adenosylethionine showed competitive inhibition. Under conditions of inhibition of protein synthesis by cycloheximide or methionine starvation, permease activity was stable. The mutant\n            <jats:italic>sam-p3<\/jats:italic>\n            apparently was able to transport\n            <jats:italic>S<\/jats:italic>\n            -adenosylmethionine only by diffusion. Uptake by diploids containing this mutation was directly proportional to the gene dose.\n          <\/jats:p>","DOI":"10.1128\/jb.109.2.499-504.1972","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T13:38:30Z","timestamp":1578058710000},"page":"499-504","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Transport of\n            <i>S<\/i>\n            -Adenosylmethionine in\n            <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1128","volume":"109","author":[{"given":"J. T.","family":"Murphy","sequence":"first","affiliation":[{"name":"Department of Bacteriology and Public Health, Washington State University, Pullman, Washington 99163"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. D.","family":"Spence","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Public Health, Washington State University, Pullman, Washington 99163"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.109.2.499-504.1972","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.109.2.499-504.1972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:01:04Z","timestamp":1627578064000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.109.2.499-504.1972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1972,2]]}},"alternative-id":["10.1128\/jb.109.2.499-504.1972"],"URL":"https:\/\/doi.org\/10.1128\/jb.109.2.499-504.1972","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,2]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}