{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T17:12:44Z","timestamp":1763226764829},"reference-count":24,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1973,2,1]],"date-time":"1973-02-01T00:00:00Z","timestamp":97372800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1973,2]]},"abstract":"<jats:p>\n            This report describes the ultrastructural features of\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            after freeze-etching of intact cells and enzymatically prepared spheroplasts. Freeze-etching of intact cells revealed two convex layers of the cell wall and particles within the hydrophobic interior of the cell membrane. Areas of the membrane free of particles were sometimes elevated in the form of rather large dome-shaped structures. Spheroplasts were formed from intact cells by the addition of trypsin to a reaction mixture of lysozyme and ethylenediaminetetraacetic acid. Spheroplasts contained the outer lipoid layer of the cell wall. It was possible to observe this cell wall layer in freeze-etch preparations of spheroplasts. The spheroplast membrane like that of intact cells was cleaved along a central plane to expose particles and particle-free areas.\n          <\/jats:p>","DOI":"10.1128\/jb.113.2.963-968.1973","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:41:14Z","timestamp":1578062474000},"page":"963-968","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Surface Structure of Intact Cells and Spheroplasts of\n            <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"113","author":[{"given":"Richard L.","family":"Weiss","sequence":"first","affiliation":[{"name":"Department of Microbiology, Indiana University, Bloomington, Indiana 47401"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dean","family":"Fraser","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Indiana University, Bloomington, Indiana 47401"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1128\/JB.92.2.380-387.1966","article-title":"Role of multivalent cations in the organization, structure and assembly of the cell wall of Pseudomonas aeruginosa","volume":"92","author":"Asbell M. 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