{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T09:38:10Z","timestamp":1705052290258},"reference-count":30,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1973,8,1]],"date-time":"1973-08-01T00:00:00Z","timestamp":113011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1973,8]]},"abstract":"<jats:p>\n            Purified minicells of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            K-12 containing the plasmid Col-\n            <jats:italic>trp<\/jats:italic>\n            <jats:sup>+<\/jats:sup>\n            or Col-\n            <jats:italic>trpA2<\/jats:italic>\n            could be derepressed for the synthesis of anthranilate synthase, the first enzyme encoded in the\n            <jats:italic>trp<\/jats:italic>\n            operon. Non-plasmid-containing, deoxyribonucleic acid-deficient minicells could not be derepressed. Derepressed enzyme synthesis was initiated by\n            <jats:sc>l<\/jats:sc>\n            -tryptophan starvation. The kinetics of derepression were studied with minicells containing the Col-\n            <jats:italic>trpA2<\/jats:italic>\n            plasmid. The derepression curves were biphasic with a rapid initial rate of enzyme synthesis followed by a slower rate of synthesis. The presence of\n            <jats:sc>l<\/jats:sc>\n            -tryptophan (20 to 50 \u03bcg\/ml) or chloramphenicol (200 \u03bcg\/ml) abolished enzyme synthesis. The presence of rifamycin SV (280 \u03bcg\/ml) partially inhibited enzyme synthesis after at least 3.5 min of exposure. The ratio of minicell-to-cell synthetic capacity was 1:2.4 when compared on the basis of derepressed enzyme activity per unit cell volume. This work demonstrates that plasmid-containing minicells are capable of considerable functional protein and messenger ribonucleic acid synthesis and that the regulation of at least the\n            <jats:italic>trp<\/jats:italic>\n            operon is similar in minicells to that observed in cells.\n          <\/jats:p>","DOI":"10.1128\/jb.115.2.615-622.1973","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:53:23Z","timestamp":1578063203000},"page":"615-622","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Derepression of Anthranilate Synthase in Purified Minicells of\n            <i>Escherichia coli<\/i>\n            Containing the Col-\n            <i>trp<\/i>\n            Plasmid"],"prefix":"10.1128","volume":"115","author":[{"given":"Anne Cornish","family":"Frazer","sequence":"first","affiliation":[{"name":"Biology Division, Oak Ridge National Laboratory and University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tennessee 37830"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Roy","family":"Curtiss","sequence":"additional","affiliation":[{"name":"Biology Division, Oak Ridge National Laboratory and University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tennessee 37830"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1073\/pnas.57.2.321","article-title":"Miniature Escherichia coli cells deficient in DNA","volume":"57","author":"Adler H. I.","year":"1967","unstructured":"Adler , H. I. , W. D. Fisher , A. Cohen , and A. A. Hardigree . 1967 . Miniature Escherichia coli cells deficient in DNA . Proc. Nat. Acad. Sci. U.S.A. 57 : 321 - 326 .","journal-title":"Proc. Nat. Acad. Sci. U.S.A."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1073\/pnas.60.1.313","article-title":"The periodicity of RNA polymerase initiations: a new regulatory feature of transcription","volume":"60","author":"Baker R. F.","year":"1968","unstructured":"Baker , R. F. , and C. Yanofsky . 1968 . The periodicity of RNA polymerase initiations: a new regulatory feature of transcription . Proc. Nat. Acad. Sci. U.S.A. 60 : 313 - 320 .","journal-title":"Proc. Nat. Acad. Sci. U.S.A."},{"key":"p_3","first-page":"5577","article-title":"Anthranilate synthetase, partial purification and some kinetic studies on the enzyme from E. coli","volume":"241","author":"Baker T. I.","year":"1966","unstructured":"Baker , T. I. , and I. P. Crawford . 1966 . Anthranilate synthetase, partial purification and some kinetic studies on the enzyme from E. coli . J. Biol. Chem. 241 : 5577 - 5584 .","journal-title":"J. Biol. Chem."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1101\/SQB.1968.033.01.071","article-title":"The properties of DNA transferred to minicells during conjugation","volume":"33","author":"Cohen A.","year":"1968","unstructured":"Cohen , A. , W. D. Fisher , R. Curtiss , and H. I. Adler . 1968 . The properties of DNA transferred to minicells during conjugation . Cold Spring Harbor Symp. Quant. Biol. 33 : 635 - 641 .","journal-title":"Cold Spring Harbor Symp. Quant. Biol."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/j.1749-6632.1971.tb30655.x","article-title":"Studies on the molecular nature of R factors","volume":"182","author":"Cohen S. N.","year":"1971","unstructured":"Cohen , S. N. , R. P. Silver , P. A. Sharp , and A. E. McCoubrey . 1971 . 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