{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:33:53Z","timestamp":1790314433721,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1973,8,1]],"date-time":"1973-08-01T00:00:00Z","timestamp":113011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1973,8]]},"abstract":"<jats:p>\n            Hydroxyurea, a potent inhibitor of deoxyribonucleic acid synthesis, inhibits the development of trachoma agent when applied at a concentration of 5 \u00d7 10\n            <jats:sup>\u22122<\/jats:sup>\n            M. At a lower concentration, 5 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            M, hydroxyurea permits the development of the trachoma inclusion bodies and initial bodies, but arrests the formation of elementary bodies, the infectious entity of the agent. The inhibitory effect of 5 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            M hydroxyurea is reversible and can be used to synchronize the development of the agent. The synthesis of deoxyribonucleic acid, ribonucleic acid, and proteins takes place in the initial bodies after the removal of the inhibitor.\n          <\/jats:p>","DOI":"10.1128\/jb.115.2.682-690.1973","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:54:15Z","timestamp":1578063255000},"page":"682-690","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Studies on the Developmental Cycle of\n            <i>Chlamydia trachomatis<\/i>\n            : Selective Inhibition by Hydroxyurea"],"prefix":"10.1128","volume":"115","author":[{"given":"Herbert S.","family":"Rosenkranz","sequence":"first","affiliation":[{"name":"Laboratory for Molecular Virology, Department of Virology, Institute of Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bezalel","family":"Gutter","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular Virology, Department of Virology, Institute of Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yechiel","family":"Becker","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular Virology, Department of Virology, Institute of Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1128\/JB.109.3.966-970.1972","article-title":"Synthesis of trachoma agent proteins in emetine-treated cells","volume":"109","author":"Becker Y.","year":"1972","unstructured":"Becker , Y. , and Y. Asher . 1972 . Synthesis of trachoma agent proteins in emetine-treated cells . J. Bacteriol. 109 : 966 - 970 .","journal-title":"J. Bacteriol."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1128\/jb.96.4.1214-1224.1968","article-title":"Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B\/r","volume":"96","author":"Clark D. J.","year":"1968","unstructured":"Clark , D. J. 1968 . Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B\/r . J. Bacteriol. 96 : 1214 - 1224 .","journal-title":"J. Bacteriol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/JB.100.1.260-268.1969","article-title":"Control of cell division in Escherichia coli: experiments with thymine starvation","volume":"100","author":"Donachie W. D.","year":"1969","unstructured":"Donachie , W. D. 1969 . Control of cell division in Escherichia coli: experiments with thymine starvation . J. Bacteriol 100 : 260 - 268 .","journal-title":"J. Bacteriol"},{"key":"p_4","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1016\/0006-2952(64)90186-8","article-title":"Effect of hydroxyurea on the incorporation of thymidine into Ehrlich ascites tumor cells","volume":"13","author":"Gale G. R.","year":"1964","unstructured":"Gale , G. R. 1964 . Effect of hydroxyurea on the incorporation of thymidine into Ehrlich ascites tumor cells . Biochem. Pharmacol. 13 : 1377 - 1382 .","journal-title":"Biochem. Pharmacol."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1432-1033.1971.tb01601.x","article-title":"Effect of emetine on ribonucleic acid synthesis in HeLa cells","volume":"23","author":"Gilead Z.","year":"1971","unstructured":"Gilead , Z. , and Y. Becker . 1971 . Effect of emetine on ribonucleic acid synthesis in HeLa cells . Eur. J. Biochem. 23 : 143 - 149 .","journal-title":"Eur. J. Biochem."},{"key":"p_6","first-page":"4089","article-title":"Inhibitors of protein synthesis. Effects of emetine on protein and nucleic acid biosynthesis in HeLa cells","volume":"243","author":"Grollman A. P.","year":"1968","unstructured":"Grollman , A. P. 1968 . Inhibitors of protein synthesis. Effects of emetine on protein and nucleic acid biosynthesis in HeLa cells . J. Biol. Chem. 243 : 4089 - 4094 .","journal-title":"J. Biol. Chem."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1128\/jb.115.2.691-702.1973","article-title":"Studies on the developmental cycle of trachoma agent: isolation and characterization of the initial bodies","volume":"115","author":"Gutter B.","year":"1973","unstructured":"Gutter , B. , Asher, Y. , Y. Cohen , and Y. Becker . 1973 . Studies on the developmental cycle of trachoma agent: isolation and characterization of the initial bodies . J. Bacteriol. 115 : 691 - 702 .","journal-title":"J. Bacteriol."},{"key":"p_8","doi-asserted-by":"crossref","unstructured":"Gutter B. and Y. Becker. 1972. Synthesis and maturation of ribosomal RNA during the developmental cycle of trachoma agent a prokaryotic obligate parasite of O '4C-EB RNA marker; *- * cells labeled for 2 h;","DOI":"10.1016\/0022-2836(72)90476-7"},{"key":"p_9","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1111\/j.1601-5223.1966.tb02057.x","article-title":"Effects of hydroxyurea on chromosomes, cell division and nucleic and synthesis in Vicia faba","volume":"55","author":"Kihlman B. A.","year":"1966","unstructured":"Kihlman , B. A. , T. Eriksson , and G. Odmark . 1966 . Effects of hydroxyurea on chromosomes, cell division and nucleic and synthesis in Vicia faba . Hereditas 55 : 386 - 397 .","journal-title":"Hereditas"},{"key":"p_10","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1128\/jb.96.4.1421-1423.1968","article-title":"Lack of deoxyribonucleic acid homology between species of the genus chlamydia","volume":"96","author":"Kingsbury D. T.","year":"1968","unstructured":"Kingsbury , D. T. , and E. Weiss . 1968 . Lack of deoxyribonucleic acid homology between species of the genus chlamydia . J. Bacteriol. 96 : 1421 - 1423 .","journal-title":"J. Bacteriol."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1128\/IAI.3.2.333-341.1971","article-title":"Ultrastructural analysis of the effects of penicillin and chlorotetracyline on the development of a genital tract chlamydia","volume":"3","author":"Kramer J. M.","year":"1971","unstructured":"Kramer , J. M. , and F. B. Gordon . 1971 . Ultrastructural analysis of the effects of penicillin and chlorotetracyline on the development of a genital tract chlamydia . Infect. Immunity 3 : 333 - 341 .","journal-title":"Infect. Immunity"},{"key":"p_12","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1042\/bj1020251","article-title":"The fractionation of high-molecular weight RNA by polyacrylamide gel electrophoresis","volume":"102","author":"Loening E. F.","year":"1967","unstructured":"Loening E. F. 1967 . The fractionation of high-molecular weight RNA by polyacrylamide gel electrophoresis . Biochem. J. 102 : 251 - 257 .","journal-title":"Biochem. J."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1128\/JB.91.2.823-833.1966","article-title":"Effect of hydroxyurea on virus development. I. Electron microscopic study of the effect on the development of bacteriophage T4","volume":"91","author":"Margaretten W.","year":"1966","unstructured":"Margaretten W. , C. Morgan , H. S. Rosenkranz , and H. M. Rose . 1966 . Effect of hydroxyurea on virus development. I. Electron microscopic study of the effect on the development of bacteriophage T4 . J. Bacteriol. 91 : 823 - 833 .","journal-title":"J. Bacteriol."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1128\/JVI.2.10.1163-1171.1968","article-title":"Electron microscopy of herpes simplex virus. III. Effect of hydroxyurea","volume":"2","author":"Nii S.","year":"1968","unstructured":"Nii , S. , H. S. Rosenkranz , C. Morgan , and H. M. Rose . 1968 . Electron microscopy of herpes simplex virus. III. Effect of hydroxyurea . J. Virol. 2 : 1163 - 1171 .","journal-title":"J. Virol."},{"key":"p_15","first-page":"186","article-title":"Amino acid requirements of trachoma strains and other agents of the PLT group in cell cultures","volume":"1","author":"Ossowski L.","year":"1965","unstructured":"Ossowski L. , Y. Becker , and H. Bernkopf . 1965 . Amino acid requirements of trachoma strains and other agents of the PLT group in cell cultures . Israel J. Med. Sci. 1 : 186 - 193 .","journal-title":"Israel J. Med. Sci."},{"key":"p_16","first-page":"1214","article-title":"Metabolic effects of hydroxyurea on BHK21 cells transformed with polyoma virus","volume":"27","author":"Pollak R. D.","year":"1967","unstructured":"Pollak , R. D. , and H. S. Rosenkranz . 1967 . Metabolic effects of hydroxyurea on BHK21 cells transformed with polyoma virus . Cancer Res. 27 : 1214 - 1224 .","journal-title":"Cancer Res."},{"key":"p_17","first-page":"228","article-title":"Studies with hydroxyurea. VI. Effects of hydroxyurea on the metabolism of sensitive and resistant strains of Escherichia coli","volume":"194","author":"Rosenkranz H. S.","year":"1967","unstructured":"Rosenkranz , H. S. , H. S. Carr , and R. D. Pollak . 1967 . Studies with hydroxyurea. VI. Effects of hydroxyurea on the metabolism of sensitive and resistant strains of Escherichia coli . Biochem. Biophys. Acta 194 : 228 - 245 .","journal-title":"Biochem. Biophys. Acta"},{"key":"p_18","first-page":"501","article-title":"Studies with hydroxyurea. I. the reversible inhibition of bacterial DNA synthesis and the effect of hydroxyurea on the bacterial action of streptomycin","volume":"114","author":"Rosenkranz H. S.","year":"1966","unstructured":"Rosenkranz , H. S. , A. J. Garro , J. A. Levy , and H. S. Carr . 1966 . Studies with hydroxyurea. I. the reversible inhibition of bacterial DNA synthesis and the effect of hydroxyurea on the bacterial action of streptomycin . Biochim. Biophys. Acta 114 : 501 - 515 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_19","first-page":"15","article-title":"Inhibition of DNA synthesis by hydroxyurea","volume":"6","author":"Rosenkranz H. S.","year":"1968","unstructured":"Rosenkranz , H. S. , and S. J. Jacobs . 1968 . Inhibition of DNA synthesis by hydroxyurea . Gann. Monog. 6 : 15 - 41 .","journal-title":"Gann. Monog."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0005-2787(65)90225-X","article-title":"Hydroxyurea: a specific inhibitor of deoxyribonucleic acid synthesis","volume":"95","author":"Rosenkranz H. S.","year":"1965","unstructured":"Rosenkranz H. S. , and J. A. Levy . 1965 . Hydroxyurea: a specific inhibitor of deoxyribonucleic acid synthesis . Biochim. Biophys. Acta 95 : 181 - 183 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_21","first-page":"209","article-title":"Biologic effects of isohydroxyurea","volume":"29","author":"Rosenkranz H. S.","year":"1969","unstructured":"Rosenkranz , H. S. , R. D. Pollak , and R. M. Schmidt . 1969 . Biologic effects of isohydroxyurea . Cancer Res. 29 : 209 - 218 .","journal-title":"Cancer Res."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/0042-6822(66)90235-2","article-title":"The effect of hydroxyurea on virus development. II. Vaccinia virus","volume":"28","author":"Rosenkranz H. S.","year":"1961","unstructured":"Rosenkranz , H. S. , H. M. Rose , C. Morgan , and K. C. Hsu . 1961 . The effect of hydroxyurea on virus development. II. Vaccinia virus . Virology 28 : 510 - 519 .","journal-title":"Virology"},{"key":"p_23","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/0022-2836(69)90245-9","article-title":"Trachoma agent DNA","volume":"42","author":"Sarov I.","year":"1969","unstructured":"Sarov , I. , and Y. Becker . 1969 . Trachoma agent DNA , J. Mol. Biol. 42 : 581 - 589 .","journal-title":"J. Mol. Biol."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1128\/JB.93.6.2003-2008.1967","article-title":"Purification and chemical composition of reticulate bodies of the meningopneumonitis organisms","volume":"93","author":"Tamura A.","year":"1967","unstructured":"Tamura , A. , A. Matsumoto , and N. Higashi . 1967 . Purification and chemical composition of reticulate bodies of the meningopneumonitis organisms . J. Bacteriol. 93 : 2003 - 2008 .","journal-title":"J. Bacteriol."},{"key":"p_25","unstructured":"Weiss E. 1971. Evolution of chlamydia p. 3-12. In R. L. Ncihols (ed.) Trachoma and related disorders caused by chlamydial agents. Exerpta Medica Foundation. Amsterdam."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1126\/science.146.3648.1172","article-title":"Hydroxyurea: inhibitory effect on DNA metabolism","volume":"146","author":"Young C. W.","year":"1964","unstructured":"Young , C. W. , and S. Hodas . 1964 . Hydroxyurea: inhibitory effect on DNA metabolism . Science 146 : 1172 - 1174 .","journal-title":"Science"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.115.2.682-690.1973","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.115.2.682-690.1973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:10:30Z","timestamp":1627578630000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.115.2.682-690.1973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,8]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1973,8]]}},"alternative-id":["10.1128\/jb.115.2.682-690.1973"],"URL":"https:\/\/doi.org\/10.1128\/jb.115.2.682-690.1973","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,8]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}