{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:53:26Z","timestamp":1761897206460},"reference-count":20,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1973,11,1]],"date-time":"1973-11-01T00:00:00Z","timestamp":120960000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1973,11]]},"abstract":"<jats:p>\n            A mutant of\n            <jats:italic>Neurospora crassa<\/jats:italic>\n            (\n            <jats:italic>cni<\/jats:italic>\n            -1) has been isolated that has two pathways of mitochondrial respiration. One pathway is sensitive to cyanide and antimycin A, the other is sensitive only to salicyl hydroxamic acid. Respiration can proceed through either pathway and both pathways together in this mutant account for greater than 90% of all mitochondrial respiration. The\n            <jats:italic>cni<\/jats:italic>\n            -1 mutation segregates as a nuclear gene in crosses to other strains of\n            <jats:italic>Neurospora<\/jats:italic>\n            . Absorption spectra of isolated mitochondria from\n            <jats:italic>cni<\/jats:italic>\n            -1 show typical\n            <jats:italic>b<\/jats:italic>\n            - and\n            <jats:italic>c<\/jats:italic>\n            -type cytochromes but the absorption peaks corresponding to cytochrome\n            <jats:italic>aa<\/jats:italic>\n            <jats:sub>3<\/jats:sub>\n            are not detectable. Extraction of soluble cytochrome\n            <jats:italic>c<\/jats:italic>\n            -546 from these mitochondria followed by reduction with ascorbate reveals a new absorption peak at 426 nm that is not present in wild-type mitochondria. This peak may be due to an altered cytochrome oxidase with abnormal spectral properties. Mitochondria from\n            <jats:italic>cni<\/jats:italic>\n            -1 have elevated levels of succinate-cytochrome\n            <jats:italic>c<\/jats:italic>\n            reductase but reduced levels of nicotinamide adenine dinucleotide reduced form cytochrome\n            <jats:italic>c<\/jats:italic>\n            reductase and of cyanide- and azide-sensitive cytochrome\n            <jats:italic>c<\/jats:italic>\n            oxidase. These studies suggest that the\n            <jats:italic>cni<\/jats:italic>\n            -1 mutation results in the abnormal assembly of cytochrome\n            <jats:italic>c<\/jats:italic>\n            oxidase so that the typical cytochrome\n            <jats:italic>aa<\/jats:italic>\n            <jats:sub>3<\/jats:sub>\n            spectrum is lost and the enzyme activity is reduced. As a consequence of this alteration, a cyanide-insensitive respiratory pathway is elaborated by these mitochondria which may serve to stimulate adenosine 5\u2032-triphosphate production via substrate level phosphorylation by glycolysis and the Krebs cycle.\n          <\/jats:p>","DOI":"10.1128\/jb.116.2.610-618.1973","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:55:22Z","timestamp":1578063322000},"page":"610-618","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Altered Mitochondrial Respiration in a Chromosomal Mutant of\n            <i>Neurospora crassa<\/i>"],"prefix":"10.1128","volume":"116","author":[{"given":"David L.","family":"Edwards","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, State University of New York, Albany, New York 12222"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabia","family":"Kwiecinski","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, State University of New York, Albany, New York 12222"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1093\/genetics\/71.4.521","article-title":"Isolation and classification of extranuclear mutants of Neurospora crassa","volume":"71","author":"Bertrand H.","year":"1972","unstructured":"Bertrand , H. , and T. 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