{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T23:40:15Z","timestamp":1649029215797},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1974,2,1]],"date-time":"1974-02-01T00:00:00Z","timestamp":128908800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1974,2]]},"abstract":"<jats:p>\n            Oxidative phosphorylation has been demonstrated with mitochondria of the mi-1 respiratory mutant of\n            <jats:italic>Neurospora crassa<\/jats:italic>\n            . The P\/O ratios observed with these mitochondria were approximately 0.8 with citrate and 0.4 with either externally added reduced nicotinamide adenine dinucleotide (NADH), succinate, or ascorbate-tetramethyl-\n            <jats:italic>p<\/jats:italic>\n            -phenylenediamine (TPD). These P\/O ratios suggest that there are only two sites of phosphorylation in mitochondria isolated from young (20 to 24 h) cultures of the mi-1 mutant. The energy-dependent reduction of NAD\n            <jats:sup>+<\/jats:sup>\n            with succinate and the phosphorylation associated with ascorbate-TPD oxidation indicate that the first and the third sites of energy coupling are present in this mutant. Difference spectra of mitochondria from young cultures of the mi-1 mutant revealed the presence of cytochrome\n            <jats:italic>c<\/jats:italic>\n            . Cytochromes\n            <jats:italic>b<\/jats:italic>\n            and\n            <jats:italic>a<\/jats:italic>\n            +\n            <jats:italic>a<\/jats:italic>\n            <jats:sub>3<\/jats:sub>\n            were not detected. However, in the presence of antimycin A, a small peak in the Soret region at 430 nm was observed. A carbon monoxide difference spectrum revealed the presence of a component of the respiratory chain with a spectrum similar to that of cytochrome\n            <jats:italic>o<\/jats:italic>\n            . It is of interest that respiratory inhibitors such as antimycin A, 2-\n            <jats:italic>n<\/jats:italic>\n            -nonylhydroxyquinoline\n            <jats:italic>N<\/jats:italic>\n            -oxide, and cyanide abolished phosphorylation but only partially inhibited oxidation. It is postulated that the mi-1 respiratory system contains two pathways of electron transport\u2014the first is associated with a phosphorylating pathway, whereas the second is a non-phosphorylating electron transport pathway.\n          <\/jats:p>","DOI":"10.1128\/jb.117.2.733-740.1974","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:59:21Z","timestamp":1578063561000},"page":"733-740","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Studies of Respiratory Components and Oxidative Phosphorylation in Mitochondria of mi-1\n            <i>Neurospora crassa<\/i>"],"prefix":"10.1128","volume":"117","author":[{"given":"Alicja","family":"Drabikowska","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Southern California School of Medicine, Los Angeles, California 90033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank C.","family":"Kosmakos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Southern California School of Medicine, Los Angeles, California 90033"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnold F.","family":"Brodie","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Southern California School of Medicine, Los Angeles, California 90033"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1016\/S0021-9258(18)70055-0","article-title":"Photochemical determinations of the oxidases of bacteria","volume":"234","author":"Castor L. 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