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The LPS extracted from iron-grown cells (Fe-LPS) contained less than 3% protein compared to 18 to 25% in LPS extracted from either sulfur-grown cells (S-LPS) or glucose-grown cells (G-LPS). S-LPS showed two distinct sedimentable species, 61S and 9.3S, which could be fractionated on a column of Sepharose 4B. The relative densities of both S-LPS and G-LPS were found to be significantly greater than that of Fe-LPS. Spectral differences were noted when each LPS was reacted with a carbocyanine dye. Fe-LPS showed a single absorbance maximum at 472 nm, S-LPS displayed its maximum at 650 nm, and G-LPS showed two maxima, the first at 468 nm and the other at 655 nm. Analysis of the methyl ester derivatives of the LPS fatty aicds using gas chromatography-mass spectrometry revealed the presence of a very stable species, tentatively identified as a methoxy methyl ester with a formula of CH3-3-C10H10-COOCH3, as the major component from each LPS. beta-Hydroxymyristic acid was found only in Fe-LPS.<\/jats:p>","DOI":"10.1128\/jb.123.2.642-650.1975","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:48:30Z","timestamp":1578066510000},"page":"642-650","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Physical and chemical studies of Thiobacillus ferroxidans lipopolysaccharides"],"prefix":"10.1128","volume":"123","author":[{"given":"W E","family":"Hirt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J R","family":"Vestal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1139\/m69-048","article-title":"Extracellular complex from the culture filtrate of Ferrobacillus ferrooxidans","volume":"15","author":"Agate A. 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