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One hundred- to 200-fold accumulation of the amino acid was attained in the presence of both electrical and chemical driving forces for the sodium ion. Somewhat lower accumulation values were obtained when either chemical or electrical driving forces were applied separately. A chemical driving force was produced by the addition of external Na+ to Na+-free cells. A membrane potential was established by a diffusion potential either of H+ in the presence of carbonyl cyanide p-trifluoromethoxyphenylhydrazone or of SCN-. These results support the hypothesis of a Na+-glutamate cotransport. Na+-driven glutamate transport was also observed in wild-type E. coli B but not in a strain of K-12.<\/jats:p>","DOI":"10.1128\/jb.131.3.848-853.1977","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T15:58:57Z","timestamp":1578067137000},"page":"848-853","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Glutamate transport driven by an electrochemical gradient of sodium ions in Escherichia coli"],"prefix":"10.1128","volume":"131","author":[{"given":"T","family":"Tsuchiya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S M","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Raven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1073\/pnas.70.5.1514","article-title":"Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli","volume":"70","author":"Berger E. 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