{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T16:15:22Z","timestamp":1648829722957},"reference-count":28,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1980,6,1]],"date-time":"1980-06-01T00:00:00Z","timestamp":328665600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1980,6]]},"abstract":"<jats:p>\n            A variety of degradative treatments have been used to investigate the nature of the structure and components of the cell walls of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            B. The binding and localization of the endotoxin-like particles found on the cell walls were of special interest because some of them are associated with the site where the inner tail tube of bacteriophage T4D penetrates the cell wall. Modified cell walls were obtained by heating a suspension of bacterial cells originally in 0.1 M phosphate, pH 7.0, after the addition of 12.5 M NaOH to a final concentration of 0.25 M. With regard to the endotoxin-like particles, it was found that: (i) at least part of them still remained bound to the modified cell wall after the alkali treatment; (ii) the subsequent incubation of alkali-treated cell walls with lysozyme destroyed the bacterial form and released a complex of endotoxin-like particles together with a fibrous material; (iii) on the other hand, treatment with 45% phenol at 70\u00b0C removed the endotoxin-like particles from the surface of the alkali-treated cell walls, but most of the fibrous material was left on the cell wall; and (iv) incubation of alkali-treated cell walls with 5 mM ethylenediaminetetraacetic acid at 20\u00b0C also removed the endotoxin-like particles, but did not disrupt the rodlike bacterial form. However, if the ethylenediaminetetraacetic acid treatment was performed at 55\u00b0C, the bacterium-like form was destroyed. These differential sensitivities to ethylenediaminetetraacetic acid suggested that loosely bound divalent metal ions normally hold these endotoxin-like particles on the cell wall surface, but that probably more tightly bound metal ions are involved in the determination of cell shape. Analysis of the protein components of the alkalitreated cell walls showed that only one protein was present in significant amounts, and this protein had an electrophoretic mobility similar to that of the Braun lipoprotein. This protein was released from the alkali-treated cell walls upon heating with 2% sodium dodecyl sulfate at 100\u00b0C. Phospholipids were also absent from this structure. The distribution of the remaining cell wall components on the alkali-treated cell walls is discussed.\n          <\/jats:p>","DOI":"10.1128\/jb.142.3.982-991.1980","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T17:04:15Z","timestamp":1578071055000},"page":"982-991","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Bacteriophage T4D Receptor and the\n            <i>Escherichia coli<\/i>\n            Cell Wall Structure: Binding of Endotoxin-Like Particles to the Cell Wall"],"prefix":"10.1128","volume":"142","author":[{"given":"Jorge","family":"Zorzopulos","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80262"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"DeLong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80262"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Chapman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80262"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lloyd M.","family":"Kozloff","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80262"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1128\/JB.130.3.1364-1381.1977","article-title":"Effect of ethylenediaminetetraacetate upon the surface of Escherichia coli","volume":"130","author":"Bayer M. E.","year":"1977","unstructured":"Bayer , M. E. , and L. Leive . 1977 . Effect of ethylenediaminetetraacetate upon the surface of Escherichia coli . J. Bacteriol. 130 : 1364 - 1381 .","journal-title":"J. Bacteriol."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1128\/JB.127.3.1502-1518.1976","article-title":"Uptake and retention of metals by cell walls of Bacillus subtilis","volume":"127","author":"Beveridge T. J.","year":"1976","unstructured":"Beveridge , T. J. , and R. G. E. Murray . 1976 . Uptake and retention of metals by cell walls of Bacillus subtilis . J. Bacteriol. 127 : 1502 - 1518 .","journal-title":"J. Bacteriol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1093\/infdis\/128.Supplement_1.S9","article-title":"Molecular organization of the rigid layer and the cell wall of Escherichia coli","volume":"128","author":"Braun V.","year":"1973","unstructured":"Braun , V. 1973 . Molecular organization of the rigid layer and the cell wall of Escherichia coli . J. Infect. Dis. 128 (Suppl. ): S9 - S16","journal-title":"J. Infect. Dis."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1111\/j.1432-1033.1970.tb00301.x","article-title":"The murein-lipoprotein linkage in the cell wall of Escherichia coli","volume":"14","author":"Braun V.","year":"1970","unstructured":"Braun , V. , and H. Wolff . 1970 . The murein-lipoprotein linkage in the cell wall of Escherichia coli . Eur. J. Biochem. 14 : 387 - 391 .","journal-title":"Eur. J. Biochem."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1128\/JB.133.1.329-335.1978","article-title":"Interaction between the two major outer membrane proteins of Escherichia coli: the matrix protein and the lipoprotein","volume":"133","author":"DeMartini M.","year":"1978","unstructured":"DeMartini , M. , and M. Inouye . 1978 . Interaction between the two major outer membrane proteins of Escherichia coli: the matrix protein and the lipoprotein . J. Bacteriol. 133 : 329 - 335 .","journal-title":"J. Bacteriol."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1432-1033.1975.tb02444.x","article-title":"The major proteins of the Escherichia coli outer cell envelope membrane. Characterization of proteins II and III, comparison of all proteins","volume":"59","author":"Garten W.","year":"1975","unstructured":"Garten , W. , I. Hindennach , and U. Henning . 1975 . The major proteins of the Escherichia coli outer cell envelope membrane. Characterization of proteins II and III, comparison of all proteins . Eur. J. Biochem. 59 : 215 - 221 .","journal-title":"Eur. J. Biochem."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1128\/jb.117.1.302-311.1974","article-title":"Ultrastructural and chemical alteration of the cell envelope of Pseudomonas aeruginosa associated with resistance to ethylenediaminetetraacetate resulting from growth in a Mg 2\"-deficient medium","volume":"117","author":"Gilleland H. E.","year":"1974","unstructured":"Gilleland , H. E. , Jr. , J. D. Stinnett , and R. G. Eagon . 1974 . Ultrastructural and chemical alteration of the cell envelope of Pseudomonas aeruginosa associated with resistance to ethylenediaminetetraacetate resulting from growth in a Mg 2\"-deficient medium . J. Bacteriol. 117 : 302 - 311 .","journal-title":"J. Bacteriol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1128\/jb.113.1.417-432.1973","article-title":"Freeze-etch study of Pseudomonas aeruginosa: localization within the cell wall and an ethylenediaminetetraacetate-extractable component","volume":"113","author":"Gilleland H. E.","year":"1973","unstructured":"Gilleland , H. E. , Jr. , J. D. Stinnett , I. L. Roth , and R. G. Eagon . 1973 . Freeze-etch study of Pseudomonas aeruginosa: localization within the cell wall and an ethylenediaminetetraacetate-extractable component . J. Bacteriol. 113 : 417 - 432 .","journal-title":"J. Bacteriol."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1073\/pnas.70.7.2033","article-title":"Envelope and shape of Escherichia coli K12","volume":"70","author":"Henning U.","year":"1973","unstructured":"Henning , U. , K. Rehn , and B. Hoehn . 1973 . Envelope and shape of Escherichia coli K12 . Proc. Natl. Acad. Sci. U.S.A. 70 : 2033 - 2036 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1126\/science.156.3781.1451","article-title":"Selective release of enzymes from bacteria","volume":"156","author":"Hepple L. A.","year":"1967","unstructured":"Hepple , L. A. 1967 . Selective release of enzymes from bacteria . Science 156 : 1451 - 1455 .","journal-title":"Science"},{"key":"p_11","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1432-1033.1975.tb02443.x","article-title":"The major proteins of the Escherichia coli outer cell envelope membrane. Preparative isolation of all major membrane proteins","volume":"59","author":"Hindennach I.","year":"1975","unstructured":"Hindennach , I. , and U. Henning . 1975 . The major proteins of the Escherichia coli outer cell envelope membrane. Preparative isolation of all major membrane proteins . Eur. J. Biochem. 59 : 207 - 213 .","journal-title":"Eur. J. Biochem."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1128\/jvi.5.6.726-739.1970","article-title":"Bacteriophage tail components. I. Pteroyl polyglutamates in T-even bacteriophages","volume":"5","author":"Kozloff L. M.","year":"1970","unstructured":"Kozloff , L. M. , M. Lute , L. K. Crosby , N. Rao , V. A. Chapman , and S. S. DeLong . 1970 . Bacteriophage tail components. I. Pteroyl polyglutamates in T-even bacteriophages . J. Virol. 5 : 726 - 739 .","journal-title":"J. Virol."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"6384","DOI":"10.1016\/S0021-9258(18)93151-0","article-title":"Physical, chemical and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate","volume":"243","author":"Leive L.","year":"1968","unstructured":"Leive , L. , V. K. Shovlin , and S. E. Mergenhagen . 1968 . Physical, chemical and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate . J. Biol. Chem. 243 : 6384 - 6391 .","journal-title":"J. Biol. Chem."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1073\/pnas.61.4.1435","article-title":"An equilibrium between two fractions of lipopolysaccharide in E. coli","volume":"61","author":"Levy S. B.","year":"1968","unstructured":"Levy , S. B. , and L. Leive . 1968 . An equilibrium between two fractions of lipopolysaccharide in E. coli . Proc. Natl. Acad. Sci. U.S.A. 61 : 1435 - 1439 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_15","doi-asserted-by":"crossref","unstructured":"Nowotny A. 1971. Chemical and biological heterogeneity of endotoxins p. 309-329. In S. J. Ajl et al. (ed.) Microbial toxins vol. 4. Academic Press Inc. New York.","DOI":"10.1016\/B978-0-12-046504-0.50013-9"},{"key":"p_16","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1128\/JB.122.2.650-659.1975","article-title":"Interaction of Mg 2e with peptidoglycan and its relation to the prevention of Iysis of a marine pseudomonad","volume":"122","author":"Rayman M. K.","year":"1975","unstructured":"Rayman , M. K. , and R. A. MacLeod . 1975 . Interaction of Mg 2e with peptidoglycan and its relation to the prevention of Iysis of a marine pseudomonad . J. Bacteriol. 122 : 650 - 659 .","journal-title":"J. Bacteriol."},{"key":"p_17","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1139\/m69-130","article-title":"Characterization of a protein lipopolysaccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid","volume":"15","author":"Rogers S. W.","year":"1969","unstructured":"Rogers , S. W. , H. E. Gilleland , Jr. , and R. G. Eagon . 1969 . Characterization of a protein lipopolysaccharide complex released from cell walls of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid . Can. J. Microbiol. 15 : 743 - 748 .","journal-title":"Can. J. Microbiol."},{"key":"p_18","doi-asserted-by":"crossref","first-page":"8019","DOI":"10.1016\/S0021-9258(19)42066-8","article-title":"Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding","volume":"249","author":"Rosenbusch J. P.","year":"1974","unstructured":"Rosenbusch , J. P. 1974 . Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding . J. Biol. Chem. 249 : 8019 - 8029 .","journal-title":"J. Biol. Chem."},{"key":"p_19","first-page":"495","article-title":"The action of Iysozyme on cell walls of some Iysozyme sensitive bacteria","volume":"22","author":"Salton M. R. J.","year":"1956","unstructured":"Salton , M. R. J. 1956 . The action of Iysozyme on cell walls of some Iysozyme sensitive bacteria . Biochim. Biophys. Acta 22 : 495 - 506 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_20","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.mi.30.100176.002315","article-title":"Bacterial membrane structure. Annu","volume":"30","author":"Salton M. R. J.","year":"1976","unstructured":"Salton , M. R. J. , and P. Owen . 1976 . Bacterial membrane structure. Annu . Rev. Microbiol. 30 : 451 - 482 .","journal-title":"Rev. Microbiol."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1128\/JB.108.1.553-563.1971","article-title":"Effect of ethylenediaminetetraacetic acid, Triton X-100, and Iysozyme on the morphology and chemical composition of isolated cell walls of Escherichia coli","volume":"108","author":"Schnaitman C. A.","year":"1971","unstructured":"Schnaitman , C. A. 1971 . Effect of ethylenediaminetetraacetic acid, Triton X-100, and Iysozyme on the morphology and chemical composition of isolated cell walls of Escherichia coli . J. Bacteriol. 108 : 553 - 563 .","journal-title":"J. Bacteriol."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1083\/jcb.72.2.292","article-title":"Ultrastructure of a periodic protein layer in the outer membrane of Escherichia coli","volume":"72","author":"Steven A. C.","year":"1977","unstructured":"Steven , A. C. , B. Ten Heggeler , R. Muller , J. Kistler , and J. P. Rosenbusch . 1977 . Ultrastructure of a periodic protein layer in the outer membrane of Escherichia coli . J. Cell Biol. 72 : 292 - 301 .","journal-title":"J. Cell Biol."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1128\/JB.134.3.1089-1098.1978","article-title":"Architecture of the outer membrane of Escherichia coli. III. Protein-lipopolysaccharide complexes in intramembraneous particles","volume":"134","author":"van Alphen L.","year":"1978","unstructured":"van Alphen , L. , A. Verkleij , J. L. Leunissen-Bijvelt , and B. Lugtenberg . 1978 . Architecture of the outer membrane of Escherichia coli. III. Protein-lipopolysaccharide complexes in intramembraneous particles . J. Bacteriol. 134 : 1089 - 1098 .","journal-title":"J. Bacteriol."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0005-2736(77)90224-3","article-title":"Architecture of the outer membrane of Escherichia coli K12. II. Freeze fracture morphology of wild-type and mutant strains","volume":"466","author":"Verkleij A.","year":"1977","unstructured":"Verkleij , A. , L. van Alphen , J. Bijvelt , and B. Lugtenberg . 1977 . Architecture of the outer membrane of Escherichia coli K12. II. Freeze fracture morphology of wild-type and mutant strains . Biochim. Biophys. Acta 466 : 269 - 282 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_25","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1099\/00221287-22-1-158","article-title":"The rigid layer of the cell wall of Escherichia coli strain B","volume":"22","author":"Weidel W.","year":"1960","unstructured":"Weidel , W. , H. Frank , and H. H. Martin . 1960 . The rigid layer of the cell wall of Escherichia coli strain B . J. Gen. Microbiol. 22 : 158 - 166 .","journal-title":"J. Gen. Microbiol."},{"key":"p_26","first-page":"83","article-title":"Bacterial lipopolysaccharides. Extraction with phenolwater and further application of the procedure","volume":"5","author":"Westphal O.","year":"1965","unstructured":"Westphal , O. , and K. Jann . 1965 . Bacterial lipopolysaccharides. Extraction with phenolwater and further application of the procedure . Methods Carbohydr. Chem. 5 : 83 - 91 .","journal-title":"Methods Carbohydr. Chem."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1128\/JB.135.3.1024-1031.1978","article-title":"Reconstitution of an ordered structure from major outer membrane constituents and the lipoprotein-bearing peptidoglycan sacculus of Escherichia coli","volume":"135","author":"Yamada H.","year":"1978","unstructured":"Yamada , H. , and S. Mizushima . 1978 . Reconstitution of an ordered structure from major outer membrane constituents and the lipoprotein-bearing peptidoglycan sacculus of Escherichia coli . J. Bacteriol. 135 : 1024 - 1031 .","journal-title":"J. Bacteriol."},{"key":"p_28","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/JB.137.1.545-555.1979","article-title":"Bacteriophage T4D receptors and the Escherichia coli cell wall structure: role of spherical particles and protein b of the cell wall in bacteriophage infection","volume":"137","author":"Zorzopulos J.","year":"1979","unstructured":"Zorzopulos , J. , L. M. Kozloff , V. Chapman , and S. DeLong . 1979 . Bacteriophage T4D receptors and the Escherichia coli cell wall structure: role of spherical particles and protein b of the cell wall in bacteriophage infection . J. Bacteriol. 137 : 545 - 555 .","journal-title":"J. Bacteriol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.142.3.982-991.1980","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.142.3.982-991.1980","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:37:27Z","timestamp":1627580247000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.142.3.982-991.1980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,6]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1980,6]]}},"alternative-id":["10.1128\/jb.142.3.982-991.1980"],"URL":"https:\/\/doi.org\/10.1128\/jb.142.3.982-991.1980","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,6]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}