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These cloned determinants were further characterized by DNA-DNA hybridization experiments which also identified a third genetically unrelated tetracycline resistance determinant. Some of these genetic differences appear to represent mechanistic differences. The tetL determinant was associated with small nonconjugative plasmids and mediated resistance to tetracycline. The tetM determinant was most often \"nonplasmid\" associated and mediated resistance to minocycline as well as tetracycline. The tetN determinant was represented on a large conjugative plasmid and was genetically distinct from tetL and tetM, although phenotypically it resembled tetM.<\/jats:p>","DOI":"10.1128\/jb.149.3.995-1004.1982","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T17:30:09Z","timestamp":1578072609000},"page":"995-1004","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":170,"title":["Heterogeneity of tetracycline resistance determinants in Streptococcus"],"prefix":"10.1128","volume":"149","author":[{"given":"V","family":"Burdett","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Inamine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Rajagopalan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"p_5","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1093\/nar\/7.6.1513","article-title":"A rapid alkaline extraction procedure for screening recombinant plasmid DNA","volume":"7","author":"Birnboim H. 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