{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T14:26:11Z","timestamp":1770647171808,"version":"3.49.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1982,5,1]],"date-time":"1982-05-01T00:00:00Z","timestamp":389059200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1982,5]]},"abstract":"<jats:p>We describe two sets of plasmid-plasmid interactions in the yeast Saccharomyces cerevisiae. [HOK], [EXL], [NEX], and [KIL-k1] are genetically defined plasmids, and M1 and L are biochemically defined double-stranded RNA plasmids. We show that (i) [HOK], [NEX], and the abundance of L are related, and (ii) under submaximal curing conditions, all colonies retaining M1 also retain L. There are three pieces of evidence that either [NEX] required [HOK] for replication or [NEX] and [HOK] are on the same plasmid. The evidence is as follows. (i) The great majority of strains containing [HOK] also contain [NEX]. However, two [HOK] [NEX-o] strains do exist. (ii) Growth at 39 degrees C or growth at 34 degrees C with 3% ethanol or 2-propanol cures [HOK] and [NEX]. In a [HOK] [NEX] strain, the two plasmids are always co-cured. (iii) [HOK] and [NEX] are both maintained in mak4, mak6, and mak27 strains (mak = maintenance of [KIL-k1]), but not in mak3, mak10, and pet18 strains. Strains containing [HOK] and [NEX] have about fourfold more L double-stranded RNA than their isochromosomal, cured derivatives. In addition, a cytoductant which has acquired [HOK] and [NEX] has fourfold more L than its parent. These results are consistent with either [HOK] being a form of L or [HOK] increasing the copy number of L. Using a K1 killer strain in which L, as well as M1, could be cured by growth at 38 degrees C, we examined the distribution of loss of M1 and L under conditions giving 98% M-o colonies and at least 50% L-o colonies. No M1L-o colonies were observed, supporting the previous suggestion by others that M1 requires L.<\/jats:p>","DOI":"10.1128\/jb.150.2.545-551.1982","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T17:32:38Z","timestamp":1578072758000},"page":"545-551","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Co-curing of plasmids affecting killer double-stranded RNAs of Saccharomyces cerevisiae: [HOK], [NEX], and the abundance of L are related and further evidence that M1 requires L"],"prefix":"10.1128","volume":"150","author":[{"given":"S S","family":"Sommer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R B","family":"Wickner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_11","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/245081b0","article-title":"Preliminary characterization of two species of dsRNA in yeast and their relationship to the \"killer","volume":"245","author":"Bevan E. A.","year":"1973","unstructured":"Bevan , E. A. , A. J. Herring , and D. J. Mltchell . 1973 . Preliminary characterization of two species of dsRNA in yeast and their relationship to the \"killer \" character. Nature (London) 245 : 81 - 86 .","journal-title":"character. Nature (London)"},{"key":"p_12","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/0092-8674(80)90514-0","article-title":"Translational analysis of the killer-associated virus-like particle dsRNA genome of S. cerevisiae: M dsRNA encodes toxin","volume":"19","author":"Bostian K. A.","year":"1980","unstructured":"Bostian , K. A. , J. E. Hopper , D. T. Rogers , and D. J. Tipper . 1980 . Translational analysis of the killer-associated virus-like particle dsRNA genome of S. cerevisiae: M dsRNA encodes toxin . Celi 19 : 403 - 414 .","journal-title":"Celi"},{"key":"p_13","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1128\/jb.143.1.463-470.1980","article-title":"Encapsidation of yeast double-stranded ribonucleic acids: dependence of M on L","volume":"143","author":"Bosa K. A.","year":"1980","unstructured":"Bosa , K. A. , J. A. Sturgeon , and D. J. Tipper . 1980 . Encapsidation of yeast double-stranded ribonucleic acids: dependence of M on L . J. Bacteriol. 143 : 463 - 470 .","journal-title":"J. Bacteriol."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/0092-8674(80)90084-7","article-title":"Yeast viral double-stranded RNAs have heterogeneous 3' termini","volume":"19","author":"Bruenn J. A.","year":"1980","unstructured":"Bruenn , J. A. , and V. E. Brennan . 1980 . Yeast viral double-stranded RNAs have heterogeneous 3' termini . 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Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae . J. Gen. Virol. 22 : 387 - 394 .","journal-title":"J. Gen. Virol."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"9010","DOI":"10.1016\/S0021-9258(17)38338-2","article-title":"Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae","volume":"252","author":"Hopper J. E.","year":"1977","unstructured":"Hopper , J. E. , K. A. Bostlan , L. B. Rowe , and D. J. Tipper . 1977 . Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae . J. Biol. Chem. 252 : 9010 - 9017 .","journal-title":"J. Biol. 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