{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T20:59:23Z","timestamp":1771534763863,"version":"3.50.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1983,7,1]],"date-time":"1983-07-01T00:00:00Z","timestamp":425865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1983,7]]},"abstract":"<jats:p>Chemical analysis of the lipopolysaccharide from Rhodopseudomonas sphaeroides ATCC 17023, isolated by the phenol-chloroform-petroleum ether method, revealed the presence of glucuronic acid, 2-keto-3-deoxyoctonate, threonine, and phosphorus in the polysaccharide moiety. The lipid A component contained glucosamine, glucosamine phosphate, amide-bound 3-oxotetradecanoic acid and 3-hydroxytetradecanoic acid, and ester-bound 3-hydroxydecanoic acid and 7-tetradecenoic acid. Structural similarity of the lipid A from R. sphaeroides ATCC 17023 to enterobacterial lipid A is indicated by the existence of a serological cross-reaction occurring between the lipid A from R. sphaeroides ATCC 17023 and that from Salmonella minnesota R595. The lipopolysaccharide and lipid A of R. sphaeroides, however, were found to be neither toxic in mice nor pyrogenic in rabbits.<\/jats:p>","DOI":"10.1128\/jb.155.1.153-158.1983","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T17:44:08Z","timestamp":1578073448000},"page":"153-158","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Nontoxic lipopolysaccharide from Rhodopseudomonas sphaeroides ATCC 17023"],"prefix":"10.1128","volume":"155","author":[{"given":"W","family":"Strittmatter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Weckesser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P V","family":"Salimath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Galanos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","unstructured":"AUtorenkollektlv. 1976. Katalytische Hydrierung von Carbonylverbindungen p. 539-543. In Organikum vol. 15. VEB Deutscher Verlag der Wissenschaften Berlin."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0021-9258(17)35155-4","article-title":"A new specific color reaction of hexuronic acids","volume":"167","author":"Dische Z.","year":"1947","unstructured":"Dische , Z. 1947 . A new specific color reaction of hexuronic acids . J. Biol. Chem. 167 : 189 - 198 .","journal-title":"J. Biol. Chem."},{"key":"p_3","unstructured":"Galanos C. M. Freudenberg S. Hase F. Jay and E. Ruschmann. 1977. Biological activities and immunological properties of lipid A p. 269-276. In D. Schlessinger (ed.) Microbiology-1977. American Society for Microbiology. Washington D.C."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"5939","DOI":"10.1073\/pnas.76.11.5939","article-title":"Galactosamine-induced sensitization of the lethal effects of endotoxin","volume":"76","author":"Galanos C.","year":"1979","unstructured":"Galanos , C. , M. Freudenberg , and W. Reutter . 1979 . Galactosamine-induced sensitization of the lethal effects of endotoxin . Proc. Natl. Acad. Sci. U.S.A. 76 : 5939 - 5943 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1432-1033.1969.tb00601.x","article-title":"A new method for extraction of R lipopolysaccharides","volume":"9","author":"Galanos C.","year":"1969","unstructured":"Galanos , C. , 0. Luderitz , and 0. Westphal . 1969 . A new method for extraction of R lipopolysaccharides . Eur. J. Biochem. 9 : 245 - 249 .","journal-title":"Eur. J. Biochem."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.1432-1033.1971.tb19661.x","article-title":"Preparation and properties of antisera against the lipid A component of bacterial lipopolysaccharides","volume":"24","author":"Galanos C.","year":"1971","unstructured":"Galanos , C. , 0. Luderitz , and 0. Westphal . 1971 . Preparation and properties of antisera against the lipid A component of bacterial lipopolysaccharides . Eur. J. Biochem. 24 : 116 - 122 .","journal-title":"Eur. J. Biochem."},{"key":"p_7","first-page":"226","article-title":"Preparation and properties of a standardized lipopolysaccharide from Salmonella abortus equi (Novo Pyrexal). Zentralbl. Bakteriol. Parasitenkd","volume":"243","author":"Galanos C.","year":"1979","unstructured":"Galanos , C. , 0. Luhderitz , and 0. Westphal . 1979 . Preparation and properties of a standardized lipopolysaccharide from Salmonella abortus equi (Novo Pyrexal). Zentralbl. Bakteriol. Parasitenkd . Infektionskr. Hyg. Abt. 1 Orig. Reihe A 243 : 226 - 244 .","journal-title":"Infektionskr. Hyg. Abt. 1 Orig. Reihe A"},{"key":"p_8","unstructured":"Gaan C. O. Luderltz E. T. Rietschel and 0. Westphal. 1977. Newer aspects of the chemistry and biology of bacterial lipopolysaccharides with special reference to their lipid A component p. 239-335. In T. W. Goodwin (ed.) International review of biochemistry. Biochemistry of lipids 2 vol. 14. University Park Press Baltimore."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1128\/iai.16.2.407-412.1977","article-title":"Biological activities of lipopolysaccharides and lipid A from Rhodospirillaceae","volume":"16","author":"Galanos C.","year":"1977","unstructured":"Galanos , C. , J. Roppel , J. Weckesser , E. T. Rketschel , and H. Mayer . 1977 . Biological activities of lipopolysaccharides and lipid A from Rhodospirillaceae . Infect. Immun. 16 : 407 - 412 .","journal-title":"Infect. Immun."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1111\/j.1432-1033.1976.tb10171.x","article-title":"The linkage of Iysine in the 0-specific chains of Proteus mirabilis 1959","volume":"62","author":"Gromska W.","year":"1976","unstructured":"Gromska , W. , and H. Mayer . 1976 . The linkage of Iysine in the 0-specific chains of Proteus mirabilis 1959 . Eur. J. Biochem. 62 : 391 - 399 .","journal-title":"Eur. J. Biochem."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/bms.1200090107","article-title":"Picolinyl esters as derivatives for the structural determination of long chain branched and unsaturated fatty acids","volume":"9","author":"Harvey D. J.","year":"1982","unstructured":"Harvey , D. J. 1982 . Picolinyl esters as derivatives for the structural determination of long chain branched and unsaturated fatty acids . Biomed. Mass Spectrom. 9 : 33 - 38 .","journal-title":"Biomed. Mass Spectrom."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.1432-1033.1976.tb10212.x","article-title":"Isolation and analysis of the lipid A backbone. Lipid A structure of lipopolysaccharides from various bacterial groups","volume":"63","author":"Hase S.","year":"1976","unstructured":"Hase , S. , and E. T. Rietschel . 1976 . Isolation and analysis of the lipid A backbone. Lipid A structure of lipopolysaccharides from various bacterial groups . Eur. J. Biochem. 63 : 101 - 107 .","journal-title":"Eur. J. Biochem."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1574-6968.1981.tb06230.x","article-title":"Lipophilic lipopolysaccharide (0-antigen) in Rhodomicrobium vannielii ATCC 17100","volume":"10","author":"Hoist O.","year":"1981","unstructured":"Hoist , O. , U. Hunger , E. Gerstner , and J. Weckesser . 1981 . Lipophilic lipopolysaccharide (0-antigen) in Rhodomicrobium vannielii ATCC 17100 . FEMS Microbiol. Lett. 10 : 165 - 168 .","journal-title":"FEMS Microbiol. Lett."},{"key":"p_14","first-page":"753","article-title":"Gas chromatography of bacterial whole cell methanolysates","volume":"82","author":"Jantzen E.","year":"1974","unstructured":"Jantzen , E. , K. Bryn , and K. Bivre . 1974 . Gas chromatography of bacterial whole cell methanolysates . Acta Pathol. Microbiol. Scand. Sect. B 82 : 753 - 766 .","journal-title":"Acta Pathol. Microbiol. Scand. Sect. B"},{"key":"p_15","first-page":"595","article-title":"A new and improved microassay to determine KDO in lipopolysacharide of gram-negative bacteria. Analy","volume":"85","author":"Karkanl D.","year":"1978","unstructured":"Karkanl , D. , J. Zeltner , J. Jackson , and D. Carlo . 1978 . A new and improved microassay to determine KDO in lipopolysacharide of gram-negative bacteria. Analy . Biochem. 85 : 595 - 501 .","journal-title":"Biochem."},{"key":"p_16","doi-asserted-by":"crossref","unstructured":"Kates M. 1972. Techniques of lipidology isolation analysis and identification of lipids p. 461-515. In T. Work (ed.) Laboratory techniques. North-Holland Publishing Co. Amsterdam.","DOI":"10.1016\/S0075-7535(08)70544-8"},{"key":"p_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9258(18)71241-6","article-title":"The quantitative histochemistry of brain","volume":"207","author":"Lowry 0.","year":"1954","unstructured":"Lowry , 0. H. , N. R. Roberts , K. Y. Leiner , M. L. Wu , and A. L. Farr . 1954 . The quantitative histochemistry of brain . J. Biol. Chem. 207 : 1 - 17 .","journal-title":"J. Biol. Chem."},{"key":"p_18","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0070-2161(08)60309-3","article-title":"Lipopolysaccharides of gram-negative bacteria","volume":"17","author":"Luderltz O.","year":"1982","unstructured":"Luderltz , O. , M. Freudenberg , C. Galanos , V. Lehmann , E. T. Rietschel , and D. H. Shaw . 1982 . Lipopolysaccharides of gram-negative bacteria . Cur. Top. Membr. Transp. 17 : 79 - 151 .","journal-title":"Cur. Top. Membr. Transp."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1007\/BF00364907","article-title":"Chemical structure and biological activities of lipid A's from various bacterial -families","volume":"65","author":"Liuderitz O.","year":"1978","unstructured":"Liuderitz , O. , C. Galanos , V. Lehmann , H. Mayer , E. T. Rietschel , and J. Weckesser . 1978 . Chemical structure and biological activities of lipid A's from various bacterial -families . Naturwissenschaften 65 : 578 - 585 .","journal-title":"Naturwissenschaften"},{"key":"p_20","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/S0021-9258(18)65387-6","article-title":"A new phosphorylated intermediate in glucose oxidation","volume":"210","author":"Macgee J.","year":"1954","unstructured":"Macgee , J. , and M. Doudoroff . 1954 . A new phosphorylated intermediate in glucose oxidation . J. Biol. Chem. 210 : 617 - 626 .","journal-title":"J. Biol. Chem."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1042\/bj0280988","article-title":"A colorimetric method for the determination of N-acetyl-glucosamine and N-acetyl-chondrosamine","volume":"28","author":"Morgan W. T. J.","year":"1934","unstructured":"Morgan , W. T. J. , and L. A. Elson . 1934 . A colorimetric method for the determination of N-acetyl-glucosamine and N-acetyl-chondrosamine . Biochem. J. 28 : 988 - 995 .","journal-title":"Biochem. J."},{"key":"p_22","unstructured":"Odhan G. and S. Stenhagen. 1972. Fatty acids p. 211-228. In G. R. Waller (ed.) Biochemical application of mass spectrometry. John Wiley & Sons New York."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1073\/pnas.50.3.499","article-title":"Studies on the gram-negative cell wall. I. Evidence for the role of KDO in the lipopolysaccharide of Salmonella typhimurium","volume":"50","author":"Osborn M. J.","year":"1963","unstructured":"Osborn , M. J. 1963 . Studies on the gram-negative cell wall. I. Evidence for the role of KDO in the lipopolysaccharide of Salmonella typhimurium . Proc. Natl. Acad. Sci. U.S.A. 50 : 499 - 506 .","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"p_24","first-page":"262","article-title":"Chemical structure of lipid A","volume":"177","author":"Rketschel E. T.","year":"1977","unstructured":"Rketschel , E. T. , S. Hase , M. T. King , J. Redmond , and V. Lehmann . 1977 . Chemical structure of lipid A . Microbiology 177 : 262 - 268 .","journal-title":"Microbiology"},{"key":"p_25","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1021\/ac60231a048","article-title":"Quantitative determination of monosaccharides as their alditol acetates by gas-liquid chromatography","volume":"37","author":"Sawardeker J. S.","year":"1967","unstructured":"Sawardeker , J. S. , J. H. Sloneker , and A. Jeanes . 1967 . Quantitative determination of monosaccharides as their alditol acetates by gas-liquid chromatography . Anal. Chem. 37 : 1602 - 1604 .","journal-title":"Anal. Chem."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1128\/jb.109.3.1106-1113.1972","article-title":"Chemical analysis of and degradation studies on the cell wall lipopolysaccharide of Rhodopseudomonas capsulata","volume":"109","author":"Weckesser J.","year":"1972","unstructured":"Weckesser , J. , G. Drews , and I. Fromme . 1972 . Chemical analysis of and degradation studies on the cell wall lipopolysaccharide of Rhodopseudomonas capsulata . J. Bacteriol. 109 : 1106 - 1113 .","journal-title":"J. Bacteriol."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1128\/jb.110.1.346-353.1972","article-title":"Localization and biological and physicochemical properties of the cell wall lipopolysaccharide of Rhodopseudomonas capsulata","volume":"110","author":"Weckesser J.","year":"1972","unstructured":"Weckesser , J. , G. Drews , and R. Ladwlg . 1972 . Localization and biological and physicochemical properties of the cell wall lipopolysaccharide of Rhodopseudomonas capsulata . J. Bacteriol. 110 : 346 - 353 .","journal-title":"J. Bacteriol."},{"key":"p_28","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1146\/annurev.mi.33.100179.001243","article-title":"Lipopolysaccharides of photosynthetic prokaryotes. Annu","volume":"33","author":"Wedceaser J.","year":"1979","unstructured":"Wedceaser , J. , G. Drewa , and H. Mayer . 1979 . Lipopolysaccharides of photosynthetic prokaryotes. Annu . Rev. Microbiol. 33 : 215 - 239 .","journal-title":"Rev. Microbiol."},{"key":"p_29","unstructured":"Welnbaum G. S. Kadis and S. J. AJI (ed.). 1971. Microbial toxins vol. 4 and 5. Academic Press Inc. New York."},{"key":"p_30","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/S0021-9258(18)70158-0","article-title":"The formation of 2- keto-3-deoxy-heptonic acid in extracts of Escherichia coli B","volume":"234","author":"Welssbach A.","year":"1959","unstructured":"Welssbach , A. , and I. Hu -witz. 1959 . The formation of 2- keto-3-deoxy-heptonic acid in extracts of Escherichia coli B . J. Biol. Chem. 234 : 705 - 709 .","journal-title":"J. Biol. Chem."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.155.1.153-158.1983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.155.1.153-158.1983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:33:09Z","timestamp":1627579989000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.155.1.153-158.1983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,7]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1983,7]]}},"alternative-id":["10.1128\/jb.155.1.153-158.1983"],"URL":"https:\/\/doi.org\/10.1128\/jb.155.1.153-158.1983","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,7]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}