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The inhibition of H2 uptake by cyanide was triphasic with Ki values of 0.8, 9.9, and 93.6 microM. This result suggested that three cyanide-reactive components were involved in H2 oxidation. H2-reduced minus O2-oxidized absorption difference spectra showed peaks at 551.5 and 560 nm, indicating the involvement of c- and b-type cytochromes, respectively. This spectrum also revealed a trough at 455 nm, showing that H2 oxidation involves a flavoprotein. This flavoprotein was not reduced by H2 in the presence of cyanide. The inhibition of H2 or cytochrome c oxidation by the flavoprotein inhibitor Atebrin was monophasic; the Ki values were similar for both substrates. A role for the flavoprotein as a terminal oxidase was implicated based on its high redox potential and its sensitivity to cyanide. Cytochromes o and c-552 were identified based on their ability to bind carbon monoxide and cyanide.<\/jats:p>","DOI":"10.1128\/jb.155.2.481-487.1983","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T17:46:38Z","timestamp":1578073598000},"page":"481-487","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Involvement of cytochromes and a flavoprotein in hydrogen oxidation in Rhizobium japonicum bacteroids"],"prefix":"10.1128","volume":"155","author":[{"given":"M R","family":"O'Brian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R J","family":"Maier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1126\/science.203.4386.1255","article-title":"Hydrogenase in Rhizobium japonicum increases nitrogen fixation by nodulated soybeans","volume":"203","author":"Albrecht S. 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