{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:30:41Z","timestamp":1791916241801,"version":"4.3.4"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1983,8,1]],"date-time":"1983-08-01T00:00:00Z","timestamp":428544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1983,8]]},"abstract":"<jats:p>Pseudomonas aeruginosa PAO grown in glucose mineral salts medium released lipopolysaccharide which was chemically and immunologically similar to the cellular lipopolysaccharide. In addition, it possessed identical phage E79-inactivating properties. Through neutralization of phage activity and hemolysis inhibition assays, the organism was found to liberate lipopolysaccharide at a constant rate during log-phase growth equivalent to 1.3 to 2.2 ng\/10(8) cells over a growth temperature range of 25 to 42 degrees C. At 19 degrees C, a lipopolysaccharide was released which was deficient in phage-inactivating activity but retained its immunological properties. Chemical analysis of lipopolysaccharide extracted from cells grown at 19 degrees C showed a deficiency in the O-side-chain component fucosamine. Gel exclusion chromatography of the polysaccharide fraction derived from lipopolysaccharide isolated from cells grown at 19 degrees C exhibited a decreased content of side-chain polysaccharide as well as a difference in the hexosamine:hexose ratio. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis confirmed these results as well as establishing that an essentially normal distribution of side-chain repeating unit lengths were to be found in the 19 degrees C preparation. These results suggest a decrease in the frequency of capping R-form lipopolysaccharide at 19 degrees C.<\/jats:p>","DOI":"10.1128\/jb.155.2.817-825.1983","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T17:47:14Z","timestamp":1578073634000},"page":"817-825","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Spontaneous release of lipopolysaccharide by Pseudomonas aeruginosa"],"prefix":"10.1128","volume":"155","author":[{"given":"J E","family":"Cadieux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Kuzio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F H","family":"Milazzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A M","family":"Kropinski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"p_1","unstructured":"Adams M. H. 1959. Bacteriophages. Interscience Publishers Inc. New York."},{"key":"p_2","first-page":"109","article-title":"Electron microscopic study of Neisseria meningitidis releasing various amounts of free endotoxin","volume":"87","author":"Andersen B. M.","year":"1979","unstructured":"Andersen , B. M. , F. Skjorten , and 0. Solberg . 1979 . Electron microscopic study of Neisseria meningitidis releasing various amounts of free endotoxin . Acta Pathol. Microbiol. Scand. Sec. B , 87 : 109 - 115 .","journal-title":"Acta Pathol. Microbiol. Scand. Sec. B"},{"key":"p_3","first-page":"267","article-title":"Electron microscopic observation on Pseudomonas aeruginosa","volume":"14","author":"Baechler C. A.","year":"1974","unstructured":"Baechler , C. A. , and R. S. Berk . 1974 . Electron microscopic observation on Pseudomonas aeruginosa . Z. Allg. Mikrobiol. 14 : 267 - 281 .","journal-title":"Z. Allg. Mikrobiol."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1139\/m76-226","article-title":"Influence of temperature on the growth of lipopolysaccharide-deficient (rough) mutants of Salmonella typhimurium","volume":"22","author":"Chatterjee A. K.","year":"1976","unstructured":"Chatterjee , A. K. , K. E. Sanderson , and H. Ross . 1976 . Influence of temperature on the growth of lipopolysaccharide-deficient (rough) mutants of Salmonella typhimurium . Can. J. Microbiol. 22 : 1540 - 1548 .","journal-title":"Can. J. Microbiol."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1432-1033.1975.tb02373.x","article-title":"Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa","volume":"58","author":"Chester I. R.","year":"1975","unstructured":"Chester , I. R. , and P. M. Meadow . 1975 . Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa . Eur. J. Biochem. 58 : 273 - 282 .","journal-title":"Eur. J. Biochem."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1038\/2141052a0","article-title":"Free endotoxin","volume":"214","author":"Crutchley M. J.","year":"1967","unstructured":"Crutchley , M. J. , P. G. Marsh , and J. Cameron . 1967 . Free endotoxin . Nature (London) 214 : 1052 .","journal-title":"Nature (London)"},{"key":"p_7","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1099\/00221287-50-3-413","article-title":"Biological studies on free endotoxin and a non-toxic material from culture supernatant fluids of Escherichia coli 0.78K80","volume":"50","author":"Crutchley M. J.","year":"1968","unstructured":"Crutchley , M. J. , P. G. Marsh , and J. Cameron . 1968 . Biological studies on free endotoxin and a non-toxic material from culture supernatant fluids of Escherichia coli 0.78K80 . J. Gen. Microbiol. 50 : 413 - 420 .","journal-title":"J. Gen. Microbiol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1128\/jb.60.1.17-28.1950","article-title":"Mutants of Escherichia coli requiring methionine or vitamin B12","volume":"60","author":"Davis B. D.","year":"1950","unstructured":"Davis , B. D. , and E. S. Mingioli . 1950 . Mutants of Escherichia coli requiring methionine or vitamin B12 . J. Bacteriol. 60 : 17 - 28 .","journal-title":"J. Bacteriol."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"563","DOI":"10.4049\/jimmunol.102.3.563","article-title":"Prevention of death from endotoxin with antisera. I. The risk of fatal anaphylaxis to endotoxin","volume":"102","author":"Davis C. E.","year":"1968","unstructured":"Davis , C. E. , K. R. Brown , H. Douglas , W. J. Tate III , and A. I. Braude . 1968 . Prevention of death from endotoxin with antisera. I. The risk of fatal anaphylaxis to endotoxin . J. Immunol. 102 : 563 - 572 .","journal-title":"J. Immunol."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1084\/jem.138.5.1156","article-title":"Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis","volume":"138","author":"Devoe I. W.","year":"1973","unstructured":"Devoe , I. W. , and J. E. Gilchrist . 1973 . Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis . J. Exp. Med. 138 : 1156 - 1167 .","journal-title":"J. Exp. Med."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1021\/ac60111a017","article-title":"Colorimetric method for determination of sugars and related substances","volume":"28","author":"Dubois M.","year":"1956","unstructured":"Dubois , M. , K. A. Gilles , J. K. Hamilton , P. A. Rebers , and F. Smith . 1956 . Colorimetric method for determination of sugars and related substances . Anal. Chem. 28 : 350 - 356 .","journal-title":"Anal. Chem."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.1432-1033.1971.tb19661.x","article-title":"Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides","volume":"24","author":"Galanos C.","year":"1971","unstructured":"Galanos , C. , 0. Luderitz , and 0. Westphal . 1971 . Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides . Eur. J. Biochem. 24 : 116 - 122 .","journal-title":"Eur. J. Biochem."},{"key":"p_13","first-page":"432","article-title":"Release and somatic antigen into filtrates of an Escherichia coli broth culture","volume":"24","author":"Guckian J. C.","year":"1966","unstructured":"Guckian , J. C. , and J. E. Perry . 1966 . Release and somatic antigen into filtrates of an Escherichia coli broth culture . Tex. Rep. Biol. Med. 24 : 432 - 436 .","journal-title":"Tex. Rep. Biol. Med."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/S0021-9673(00)82770-3","article-title":"Thin-layer chromatographic method for the identification of mono, di, and trisaccharides","volume":"107","author":"Hansen S. A.","year":"1975","unstructured":"Hansen , S. A. 1975 . Thin-layer chromatographic method for the identification of mono, di, and trisaccharides . J. Chromatogr. 107 : 224 - 226 .","journal-title":"J. Chromatogr."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1128\/jvi.41.1.222-227.1982","article-title":"Polymannose 0-antigens of Escherichia coli, the binding sites for the reversible adsorption of bacteriophage T5+ via the L-shaped tail fibers","volume":"41","author":"Heller K.","year":"1982","unstructured":"Heller , K. , and V. Braun . 1982 . Polymannose 0-antigens of Escherichia coli, the binding sites for the reversible adsorption of bacteriophage T5+ via the L-shaped tail fibers . J. Virol. 41 : 222 - 227 .","journal-title":"J. Virol."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1099\/0022-1317-33-1-99","article-title":"The isolation and characterization of a lipopolysaccharide-specific Pseudomonas aeruginosa bacteriophage","volume":"33","author":"Jarrell K.","year":"1976","unstructured":"Jarrell , K. , and A. M. Kropinski . 1976 . The isolation and characterization of a lipopolysaccharide-specific Pseudomonas aeruginosa bacteriophage . J. Gen. Virol. 33 : 99 - 106 .","journal-title":"J. Gen. Virol."},{"key":"p_17","first-page":"103","article-title":"The chemical composition of the lipopolysaccharide from Pseudomonas aeruginosa strain PAO and a spontaneously derived rough mutant","volume":"19","author":"Jarrell K.","year":"1977","unstructured":"Jarrell , K. , and A. M. Kropinski . 1977 . The chemical composition of the lipopolysaccharide from Pseudomonas aeruginosa strain PAO and a spontaneously derived rough mutant . Microbios 19 : 103 - 116 .","journal-title":"Microbios"},{"key":"p_18","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1128\/jvi.23.3.461-466.1977","article-title":"Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO","volume":"23","author":"Jarrell K.","year":"1977","unstructured":"Jarrell , K. , and A. M. B. Kropinski . 1977 . Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO . J. Virol. 23 : 461 - 466 .","journal-title":"J. Virol."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1128\/jvi.38.2.529-538.1981","article-title":"Isolation and characterization of a bacteriophage specific for the lipopolysaccharide of rough derivations of Pseudomonas aeruginosa strain PAO","volume":"38","author":"Jarrell K. F.","year":"1981","unstructured":"Jarrell , K. F. , and A. M. B. Kropinski . 1981 . Isolation and characterization of a bacteriophage specific for the lipopolysaccharide of rough derivations of Pseudomonas aeruginosa strain PAO . J. Virol. 38 : 529 - 538 .","journal-title":"J. Virol."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1016\/0003-2697(71)90252-1","article-title":"Improved method of hexosamine determination","volume":"44","author":"Johnson A. R.","year":"1971","unstructured":"Johnson , A. R. 1971 . Improved method of hexosamine determination . Anal. Biochem. 44 : 628 - 635 .","journal-title":"Anal. Biochem."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1139\/m76-004","article-title":"Improved techniques for preparation of bacterial lipopolysaccharides","volume":"22","author":"Johnson K. G.","year":"1976","unstructured":"Johnson , K. G. , and M. B. Perry . 1976 . Improved techniques for preparation of bacterial lipopolysaccharides . Can. J. Microbiol. 22 : 29 - 34 .","journal-title":"Can. J. Microbiol."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1139\/m75-285","article-title":"Cellular and free lipopolysaccharides of some species of Neisseria","volume":"21","author":"Johnson K. G.","year":"1975","unstructured":"Johnson , K. G. , M. B. Perry , I. J. McDonald , and R. R. B. Russell . 1975 . Cellular and free lipopolysaccharides of some species of Neisseria . Can. J. Microbiol. 21 : 1969 - 1980 .","journal-title":"Can. J. Microbiol."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1128\/jb.92.4.1206-1217.1966","article-title":"Relation between excreted lipopolysaccharide complexes and surface structures of a Iysine-limited culture of Escherichia coli","volume":"92","author":"Knox K. W.","year":"1966","unstructured":"Knox , K. W. , M. Vesk , and E. Work . 1966 . Relation between excreted lipopolysaccharide complexes and surface structures of a Iysine-limited culture of Escherichia coli . J. Bacteriol. 92 : 1206 - 1217 .","journal-title":"J. Bacteriol."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1099\/00221287-98-2-387","article-title":"The isolation and characterization of lipopolysaccharide-defective mutants of Pseudomonas aeruginosa PAC 1","volume":"98","author":"Koval S. F.","year":"1977","unstructured":"Koval , S. F. , and P. M. Meadow . 1977 . The isolation and characterization of lipopolysaccharide-defective mutants of Pseudomonas aeruginosa PAC 1 . J. Gen. Microbiol. 98 : 387 - 398 .","journal-title":"J. Gen. Microbiol."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1128\/AAC.21.2.310","article-title":"Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa","volume":"21","author":"Kropinski A. M.","year":"1982","unstructured":"Kropinski , A. M. , J. Kuzio , B. L. Angus , and R. E. W. Hancock . 1982 . Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa . Antimicrob. Agents Chemother. 21 : 310 - 319 .","journal-title":"Antimicrob. Agents Chemother."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1139\/m79-060","article-title":"The extraction and analysis of lipopolysaccharides from Pseudomonas aeruginosa strain PAO and three rough mutants","volume":"25","author":"Kropinski A. M. B.","year":"1979","unstructured":"Kropinski , A. M. B. , L. C. Chan , and F. H. Milazzo . 1979 . The extraction and analysis of lipopolysaccharides from Pseudomonas aeruginosa strain PAO and three rough mutants . Can. J. Microbiol. 25 : 390 - 398 .","journal-title":"Can. J. Microbiol."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli U. K.","year":"1970","unstructured":"Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 .","journal-title":"Nature (London)"},{"key":"p_28","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1139\/m73-056","article-title":"The release of alkaline phosphatase and of lipopolysaccharide during growth of rough and smooth strains of Salmonella typhimurium","volume":"19","author":"Lindsay S. S.","year":"1973","unstructured":"Lindsay , S. S. , B. Wheeler , K. E. Sanderson , J. W. Costerton , and K. -J. Cheng . 1973 . The release of alkaline phosphatase and of lipopolysaccharide during growth of rough and smooth strains of Salmonella typhimurium . Can. J. Microbiol. 19 : 335 - 343 .","journal-title":"Can. J. Microbiol."},{"key":"p_29","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1099\/00221287-52-1-125","article-title":"Free endotoxin and a non-toxic material from gram-negative bacteria: electron microscopy of fractions from Escherichia coli","volume":"52","author":"Marsh D. G.","year":"1968","unstructured":"Marsh , D. G. , and P. D. Walker . 1968 . Free endotoxin and a non-toxic material from gram-negative bacteria: electron microscopy of fractions from Escherichia coli . J. Gen. Microbiol. 52 : 125 - 130 .","journal-title":"J. Gen. Microbiol."},{"key":"p_30","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1128\/jb.137.2.746-751.1979","article-title":"Variation in the structure and bacteriophage-inactivating capacity of Salmonella anatum lipopolysaccharide as a function of growth temperature","volume":"137","author":"McConnell M.","year":"1979","unstructured":"McConnell , M. , and A. Wright . 1979 . Variation in the structure and bacteriophage-inactivating capacity of Salmonella anatum lipopolysaccharide as a function of growth temperature . J. Bacteriol. 137 : 746 - 751 .","journal-title":"J. Bacteriol."},{"key":"p_31","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1099\/00221287-108-2-339","article-title":"Receptor sites for R-type pyocins and bacteriophage E79 in the core part of the lipopolysaccharide of Pseudomonas aeruginosa PAC1","volume":"108","author":"Meadow P. M.","year":"1978","unstructured":"Meadow , P. M. , and P. L. Wells . 1978 . Receptor sites for R-type pyocins and bacteriophage E79 in the core part of the lipopolysaccharide of Pseudomonas aeruginosa PAC1 . J. Gen. Microbiol. 108 : 339 - 343 .","journal-title":"J. Gen. Microbiol."},{"key":"p_32","first-page":"859","article-title":"Indirect bacterial hemagglutination and its application to the study of bacterial antigens and serological diagnosis","volume":"28","author":"Neter E.","year":"1966","unstructured":"Neter , E. 1966 . Indirect bacterial hemagglutination and its application to the study of bacterial antigens and serological diagnosis . Pathol. Microbiol. 28 : 859 - 877 .","journal-title":"Pathol. Microbiol."},{"key":"p_33","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1073\/pnas.50.3.499","article-title":"Studies on the Gram-negative cell wall. 1. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium","volume":"50","author":"Osborn M. J.","year":"1963","unstructured":"Osborn , M. J. 1963 . Studies on the Gram-negative cell wall. 1. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium . Proc. Nat. Acad. Sci. U.S.A. 50 : 499 - 506 .","journal-title":"Proc. Nat. Acad. Sci. U.S.A."},{"key":"p_34","first-page":"59","article-title":"The spontaneous release of somatic antigen from Vibrio cholerae","volume":"81","author":"Pike R. M.","year":"1974","unstructured":"Pike , R. M. , and H. Chandler . 1974 . The spontaneous release of somatic antigen from Vibrio cholerae . J. Gen. Microbiol. 81 : 59 - 67 .","journal-title":"J. Gen. Microbiol."},{"key":"p_35","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/S0003-2697(79)80035-4","article-title":"Glycoprotein detection in polyacrylamide gel with thymol and sulfuric acid","volume":"99","author":"Racusen D.","year":"1979","unstructured":"Racusen , D. 1979 . Glycoprotein detection in polyacrylamide gel with thymol and sulfuric acid . Anal. Biochem. 99 : 474 - 476 .","journal-title":"Anal. Biochem."},{"key":"p_36","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1128\/iai.15.1.34-49.1977","article-title":"Lipid A antiserummediated protection against lipopolysaccharide- and lipid A-induced fever and skin necrosis","volume":"15","author":"Reitschel E. T.","year":"1977","unstructured":"Reitschel , E. T. , and C. Galanos . 1977 . Lipid A antiserummediated protection against lipopolysaccharide- and lipid A-induced fever and skin necrosis . Infect. Immun. 15 : 34 - 49 .","journal-title":"Infect. Immun."},{"key":"p_37","first-page":"125","article-title":"Free endotoxin-a review","volume":"2","author":"Russell R. R. B.","year":"1976","unstructured":"Russell , R. R. B. 1976 . Free endotoxin-a review . Microbios Lett. 2 : 125 - 135 .","journal-title":"Microbios Lett."},{"key":"p_38","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0021-9673(00)81550-2","article-title":"Separation of amino sugars and related compounds by two-dimensional thin-layer chromatography","volume":"195","author":"Ryan E. A.","year":"1980","unstructured":"Ryan , E. A. , and A. M. Kropinski . 1980 . Separation of amino sugars and related compounds by two-dimensional thin-layer chromatography . J. Chromatog. 195 : 127 - 132 .","journal-title":"J. Chromatog."},{"key":"p_39","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/0022-2836(76)90215-1","article-title":"The adsorption of coliphage lambda to its host: effect of variations in the surface density of receptor and in phage-receptor affinity","volume":"103","author":"Schwartz M.","year":"1976","unstructured":"Schwartz , M. 1976 . The adsorption of coliphage lambda to its host: effect of variations in the surface density of receptor and in phage-receptor affinity . J. Mol. Biol. 103 : 521 - 536 .","journal-title":"J. Mol. Biol."},{"key":"p_40","doi-asserted-by":"crossref","unstructured":"Stahl E. (ed.). 1969. Thin-layer chromatography p. 817-819. Springer-Verlag New York.","DOI":"10.1007\/978-3-642-88488-7"},{"key":"p_41","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0014-5793(74)80491-6","article-title":"Correlation between pyocin sensitivity and 2-amino sugar composition of Pseudomonas aeruginosa","volume":"48","author":"Suzuki N.","year":"1974","unstructured":"Suzuki , N. 1974 . Correlation between pyocin sensitivity and 2-amino sugar composition of Pseudomonas aeruginosa . FEBS Lett. 48 : 301 - 305 .","journal-title":"FEBS Lett."},{"key":"p_42","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1042\/bj0990053","article-title":"Chemical and biological properties of an extracellular lipopolysaccharide from Escherichia coli grown under Iysine-limiting conditions","volume":"99","author":"Taylor A.","year":"1966","unstructured":"Taylor , A. , K. Knox , and E. Work . 1966 . Chemical and biological properties of an extracellular lipopolysaccharide from Escherichia coli grown under Iysine-limiting conditions . Biochem. J. 99 : 53 - 61 .","journal-title":"Biochem. J."},{"key":"p_43","article-title":"The isolation and comparison of pyrogenic factors from Proteus vulgaris","author":"Todd J. P.","year":"1958","unstructured":"Todd , J. P. , J. A. M. Shaw , J. A. Blain , and W. Boyle . 1958 . The isolation and comparison of pyrogenic factors from Proteus vulgaris . J. Pharm. Pharmacol. l(Suppl.):223T-226T.","journal-title":"J. Pharm. Pharmacol. l(Suppl.):223T-226T."},{"key":"p_44","unstructured":"Westphal O. and K. Jann. 1965. Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure p. 83-91. In R. L. Whistler (ed.) Methods in carbohydrate chemistry vol. 5. Academic Press Inc. New York."},{"key":"p_45","unstructured":"Wilkinson S. G. 1977. Composition and structure of bacterial lipopolysaccharides p. 97-175. In 1. W. Sutherland (ed.) Surface carbohydrates of the procaryote cell. Academic Press Inc. New York."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.155.2.817-825.1983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.155.2.817-825.1983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T17:54:34Z","timestamp":1695578074000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.155.2.817-825.1983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,8]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,8]]}},"alternative-id":["10.1128\/jb.155.2.817-825.1983"],"URL":"https:\/\/doi.org\/10.1128\/jb.155.2.817-825.1983","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,8]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}