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Well in advance of glucose exhaustion, a transition phase was observed, characterized by a decrease in the growth rate and a progressive reduction of protein and RNA accumulation. Two-dimensional gel analysis of the proteins synthesized during this stage showed that the pattern of proteins remained similar to that of log-phase cells. When the cells entered the stationary phase, protein accumulation was 10% of that in log-phase cells, and incorporation of labeled RNA precursor was undetectable. Analysis of protein synthesis gave evidence that the synthesis of 95% of the proteins present in log-phase cells was arrested in stationary-phase cells. Among the 20 proteins whose synthesis continues throughout the stationary phase were identified actin, aldehyde dehydrogenase, enolase, hexokinase, glyceraldehyde-3-phosphate dehydrogenase, and five heat shock proteins. In addition, the synthesis of six new proteins was observed. The occurrence of these new proteins in stationary-phase cells is presumed to result from the release of carbon catabolite repression due to glucose exhaustion.<\/jats:p>","DOI":"10.1128\/jb.161.1.385-392.1985","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T18:04:04Z","timestamp":1578074644000},"page":"385-392","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyces cerevisiae"],"prefix":"10.1128","volume":"161","author":[{"given":"H","family":"Boucherie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0092-8674(79)90150-8","article-title":"The induction of gene activity in Drosophila by heat shock","volume":"17","author":"Ashburner M.","year":"1979","unstructured":"Ashburner , M. , and J. 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