{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:13:46Z","timestamp":1791904426643,"version":"4.3.4"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1985,5,1]],"date-time":"1985-05-01T00:00:00Z","timestamp":483753600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1985,5]]},"abstract":"<jats:p>Four strains of gram-negative bacteria capable of growing at the expense of 5-chlorovanillate were isolated from soil, and the metabolism of one strain was studied in particular detail. In the presence of alpha, alpha'-bipyridyl, a suspension of 5-chlorovanillate-grown cells accumulated 5-chloroprotocatechuate from 5-chlorovanillate; in the absence of inhibitor these compounds, and various other 5-substituted protocatechuates and vanillates, were oxidized to completion. Cell suspensions of this strain grown on 5-chlorovanillate or vanillate released chloride quantitatively from 5-chlorovanillate and released methanol from syringate. Extracts of cells grown with 4-hydroxybenzoate, vanillate, or syringate possessed high levels of both protocatechuate 4,5-dioxygenase and 2-pyrone-4,6-dicarboxylate hydrolase; extracts from acetate-grown cells did not. Protocatechuate 4,5-dioxygenase, purified from strains that could grow with 5-chlorovanillate, oxidized 5-halogeno-protocatechuates and 3-O-methylgallate with the formation of 2-pyrone-4,6-dicarboxylate. A crude extract converted 5-chloroprotocatechuate into pyruvate plus oxaloacetate. On the basis of these observations, a meta-fission reaction sequence is proposed for the bacterial degradation of vanillate and protocatechuate substituted at C-5 of the benzene ring with halogen or methoxyl.<\/jats:p>","DOI":"10.1128\/jb.162.2.693-697.1985","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T16:55:27Z","timestamp":1578070527000},"page":"693-697","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Enzymatic release of halogens or methanol from some substituted protocatechuic acids"],"prefix":"10.1128","volume":"162","author":[{"given":"P J","family":"Kersten","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P J","family":"Chapman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Dagley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1042\/bj0960808","article-title":"The microbial oxidation of methanol. 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Microbiol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1432-1033.1973.tb02818.x","article-title":"Interaction of substrates with a purified 4-methoxybenzoate monooxygenase system (O-demethylating) from Pseudomonas putida","volume":"35","author":"Bernhardt F.","year":"1973","unstructured":"Bernhardt , F. - H. , N. Erdin , H. Staudinger , and V. Ullrich . 1973 . Interaction of substrates with a purified 4-methoxybenzoate monooxygenase system (O-demethylating) from Pseudomonas putida . Eur. J. Biochem. 35 : 126 - 134 .","journal-title":"Eur. J. Biochem."},{"key":"p_4","unstructured":"Chapman P. J. 1979. Degradation mechanisms p. 28-66. In A. W. Bourquin and P. H. Pritchard (ed.) Proceedings of the Workshop: Microbial Degradation of Pollutants in Marine Environments. U.S. Environmental Protection Agency Gulf Breeze Fla."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1128\/AM.26.2.176-184.1973","article-title":"Degradation of methoxylated benzoic acids by a Nocardia from a lignin-rich environment: significance to lignin degradation and effect of chloro substituents","volume":"26","author":"Crawford R. L.","year":"1973","unstructured":"Crawford , R. L. , E. McCoy , J. M. Harkin , T. K. Kirk , and J. R. Obst . 1973 . Degradation of methoxylated benzoic acids by a Nocardia from a lignin-rich environment: significance to lignin degradation and effect of chloro substituents . AppI. Microbiol. 26 : 176 - 184 .","journal-title":"AppI. Microbiol."},{"key":"p_6","doi-asserted-by":"crossref","unstructured":"Dagley S. and P. J. Chapman. 1971. Evaluation of methods used to determine metabolic pathways p. 217-268. In J. R. Norris and D. W. Ribbons (ed.) Methods in microbiology vol. 6A. Academic Press Inc. 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