{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T05:23:28Z","timestamp":1770528208495,"version":"3.49.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1985,8,1]],"date-time":"1985-08-01T00:00:00Z","timestamp":491702400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1985,8]]},"abstract":"<jats:p>A new rff mutation (rff-726) of Escherichia coli is described which affects the biosynthesis of the enterobacterial common antigen. This mutation was detected in an rfe-defective strain. A Tn10 insertion near the rfe locus was isolated to facilitate further mapping. Both mutations rfe and rff were mapped by transduction with bacteriophage P1, giving the gene order ilv rfe rff uvrD metE. The F' factor F14 was able to complement both mutations rfe and rff, whereas the F' factor F16 could complement the rfe but not the rff mutation. The rff mutation did not affect the biosynthesis of N-acetyl-D-mannosaminuronic acid, as the previously described rff mutations in Salmonella typhimurium do (H. C. Lew, H. Nikaido, and P. H. M\u00e4kel\u00e4, J. Bacteriol. 136:227-233, 1978), and also did not affect the biosynthesis of other enterobacterial common antigen components; however, the biosynthesis of the complete enterobacterial common antigen molecule was blocked.<\/jats:p>","DOI":"10.1128\/jb.163.2.756-762.1985","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T18:20:01Z","timestamp":1578075601000},"page":"756-762","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Genetic location of genes encoding enterobacterial common antigen"],"prefix":"10.1128","volume":"163","author":[{"given":"U","family":"Meier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1128\/mr.47.2.180-230.1983","article-title":"Linkage map of Escherichia coli K-12, edition 7","volume":"47","author":"Bachmann B. J.","year":"1983","unstructured":"Bachmann , B. J. 1983 . Linkage map of Escherichia coli K-12, edition 7 . Microbiol. Rev. 47 : 180 - 230 .","journal-title":"Microbiol. Rev."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1128\/jb.114.3.1264-1270.1973","article-title":"Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope","volume":"114","author":"Braun V.","year":"1973","unstructured":"Braun , V. , H. Gnirke , U. Henning , and K. Rehn . 1973 . Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope , J. Bacteriol. 114 : 1264 - 1270 .","journal-title":"J. Bacteriol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/BF00334238","article-title":"Map location of arginyl-RNA synthetase mutations in Escherichia coli K-12","volume":"104","author":"Cooper P. H.","year":"1969","unstructured":"Cooper , P. H. , I. N. Hirshfield , and W. K. Maas . 1969 . Map location of arginyl-RNA synthetase mutations in Escherichia coli K-12 . Mol. Gen. Genet. 104 : 383 - 390 .","journal-title":"Mol. Gen. Genet."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1111\/j.1432-1033.1967.tb00100.x","article-title":"4-Amino-4,6-didesoxyhexoses isolated from lipopolysaccharides of Escherichia coli","volume":"2","author":"Jann B.","year":"1967","unstructured":"Jann , B. , and K. Jann . 1967 . 4-Amino-4,6-didesoxyhexoses isolated from lipopolysaccharides of Escherichia coli . Eur. J. Biochem. 2 : 26 - 31 .","journal-title":"Eur. J. Biochem."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1099\/00221287-67-3-289","article-title":"Isolation and characterisation of Escherichia coli bacteriophage f18 specific for E. coli strains belonging to sero-group 08","volume":"67","author":"Jann K.","year":"1971","unstructured":"Jann , K. , G. Schmidt , and B. Wallenfels . 1971 . Isolation and characterisation of Escherichia coli bacteriophage f18 specific for E. coli strains belonging to sero-group 08 . J. Gen. Microbiol. 67 : 289 - 297 .","journal-title":"J. Gen. Microbiol."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1574-6968.1977.tb00633.x","article-title":"A simple method for typing of acidic polysaccharide K antigens of Escherichia coli","volume":"1","author":"Kaijser B.","year":"1977","unstructured":"Kaijser , B. 1977 . A simple method for typing of acidic polysaccharide K antigens of Escherichia coli . FEMS Microbiol. Lett. 1 : 285 - 288 .","journal-title":"FEMS Microbiol. Lett."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1093\/genetics\/90.3.427","article-title":"Properties of the translocatable tetracycline-resistance element TnlO in Escherichia coli and bacteriophage","volume":"90","author":"Kleckner N.","year":"1978","unstructured":"Kleckner , N. , D. Baker , D. Ross , and D. Botstein . 1978 . Properties of the translocatable tetracycline-resistance element TnlO in Escherichia coli and bacteriophage . Genetics 90 : 427 - 450 .","journal-title":"Genetics"},{"key":"p_8","first-page":"274","article-title":"ECA, das gemeinsame Antigen der Enterobacteriaceae-Stiefkind der Mikrobiologie","volume":"7","author":"Kuhn H. M.","year":"1984","unstructured":"Kuhn , H. M. , U. Meier , and H. Mayer . 1984 . ECA, das gemeinsame Antigen der Enterobacteriaceae-Stiefkind der Mikrobiologie . Forum Mikrobiol. 7 : 274 - 285 .","journal-title":"Forum Mikrobiol."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1128\/iai.40.2.696-700.1983","article-title":"Modification of the lipid moiety of the enterobacterial common antigen by the \"Pseudomonas factor","volume":"40","author":"Kuhn H. M.","year":"1983","unstructured":"Kuhn , H. M. , E. Neter , and H. Mayer . 1983 . Modification of the lipid moiety of the enterobacterial common antigen by the \"Pseudomonas factor .\" Infect. Immun. 40 : 696 - 700 .","journal-title":"Infect. Immun."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3181\/00379727-111-27734","article-title":"Evidence for a common hapten associated with endotoxin fractions of E. coli and other Enterobacteriaceae","volume":"111","author":"Kunin C. M.","year":"1962","unstructured":"Kunin , C. M. , M. V. Beard , and N. E. Halmagyi . 1962 . Evidence for a common hapten associated with endotoxin fractions of E. coli and other Enterobacteriaceae . Proc. Soc. Exp. Biol. Med. 111 : 160 - 166 .","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/0022-2836(74)90037-0","article-title":"Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ deletion mutants of F14","volume":"89","author":"Lee H. J.","year":"1974","unstructured":"Lee , H. J. , E. Oktsubo , R. C. Deonier , and N. Davidson . 1974 . Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ deletion mutants of F14 . J. Mol. Biol. 89 : 585 - 597 .","journal-title":"J. Mol. Biol."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1128\/jb.136.1.227-233.1978","article-title":"Biosynthesis of uridine diphosphate N-acetylmannosaminuronic acid in rff mutants of Salmonella typhimurium","volume":"136","author":"Lew H. C.","year":"1978","unstructured":"Lew , H. C. , H. Nikaido , and P. H. Maikelai . 1978 . Biosynthesis of uridine diphosphate N-acetylmannosaminuronic acid in rff mutants of Salmonella typhimurium . J. Bacteriol. 136 : 227 - 233 .","journal-title":"J. Bacteriol."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1007\/BF00364907","article-title":"Chemical structure and biological activities of lipid A's from various bacterial families","volume":"65","author":"Luderitz O.","year":"1978","unstructured":"Luderitz , O. , C. Galanos , V. Lehmann , H. Mayer , E. T. Rietschel , and J. Weckesser . 1978 . Chemical structure and biological activities of lipid A's from various bacterial families . Naturwissenschaften 65 : 578 - 585 .","journal-title":"Naturwissenschaften"},{"key":"p_14","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0008-6215(83)88045-8","article-title":"Identification of a trisaccharide repeating-unit in the enterobacterial common antigen","volume":"118","author":"Lugowski C.","year":"1983","unstructured":"Lugowski , C. , E. Romanowska , L. Kenne , and B. Lindberg . 1983 . Identification of a trisaccharide repeating-unit in the enterobacterial common antigen . Carbohydr. Res. 118 : 173 - 181 .","journal-title":"Carbohydr. Res."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1099\/00221287-60-1-91","article-title":"A new gene cluster rfe concerned with the biosynthesis of Salmonella lipopolysaccharide","volume":"60","author":"Makela P. H.","year":"1970","unstructured":"Makela , P. H. , M. Jahkola , and 0. Luderitz . 1970 . A new gene cluster rfe concerned with the biosynthesis of Salmonella lipopolysaccharide . J. Gen. Microbiol. 60 : 91 - 106 .","journal-title":"J. Gen. Microbiol."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1128\/br.40.3.591-632.1976","article-title":"Enterobacterial common antigen","volume":"40","author":"Mikeli P. H.","year":"1976","unstructured":"Mikeli , P. H. , and H. Mayer . 1976 . Enterobacterial common antigen . Bacteriol. Rev. 40 : 591 - 632 .","journal-title":"Bacteriol. Rev."},{"key":"p_17","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1128\/jb.119.3.760-764.1974","article-title":"Participation of lipopolysaccharide genes in the determination of the common enterobacterial antigen: analysis of R mutants of Salmonella minnesota","volume":"119","author":"Makelfi P. H.","year":"1974","unstructured":"Makelfi , P. H. , H. Mayer , H. Y. Whang , and E. Neter . 1974 . Participation of lipopolysaccharide genes in the determination of the common enterobacterial antigen: analysis of R mutants of Salmonella minnesota . J. Bacteriol. 119 : 760 - 764 .","journal-title":"J. Bacteriol."},{"key":"p_18","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1128\/jb.127.3.1141-1149.1976","article-title":"Enterobacterial common antigen in rjb deletion mutants of Salmonella typhimurium","volume":"127","author":"Mikela P. H.","year":"1976","unstructured":"Mikela , P. H. , G. Schmidt , H. Mayer , H. Nikaido , H. Y. Whang , and E. Neter . 1976 . Enterobacterial common antigen in rjb deletion mutants of Salmonella typhimurium . J. Bacteriol. 127 : 1141 - 1149 .","journal-title":"J. Bacteriol."},{"key":"p_19","unstructured":"Mikelfi P. H. and B. A. D. Stocker. 1984. Genetics of lipopolysaccharide p. 59-137. In E. T. Rietschel (ed.) Chemistry of endotoxins. Elsevier\/North-Holland Biomedical Press Amsterdam."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/j.1432-1033.1978.tb12318.x","article-title":"Isolation and chemical characterization of the enterobacterial common antigen","volume":"86","author":"Annel D.","year":"1978","unstructured":"M Annel , D. , and H. Mayer . 1978 . Isolation and chemical characterization of the enterobacterial common antigen . Eur. J. Biochem. 86 : 361 - 370 .","journal-title":"Eur. J. Biochem."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"2454","DOI":"10.1016\/S0021-9258(18)93874-3","article-title":"Thymidine diphosphate 4-acetamido-4,6-dideoxyhexoses. I. Enzymatic synthesis by strains of Escherichia coli","volume":"239","author":"Matsuhashi M.","year":"1964","unstructured":"Matsuhashi , M. , and J. L. Strominger . 1964 . Thymidine diphosphate 4-acetamido-4,6-dideoxyhexoses. I. Enzymatic synthesis by strains of Escherichia coli . J. Biol. Chem. 239 : 2454 - 2463 .","journal-title":"J. Biol. Chem."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1432-1033.1969.tb00506.x","article-title":"D-Mannosaminuronsaure-Baustein des K7-Antigens von Escherichia coli","volume":"8","author":"Mayer H.","year":"1969","unstructured":"Mayer , H. 1969 . D-Mannosaminuronsaure-Baustein des K7-Antigens von Escherichia coli . Eur. J. Biochem. 8 : 139 - 145 .","journal-title":"Eur. J. Biochem."},{"key":"p_23","first-page":"99","article-title":"Chemistry and biology of the enterobacterial common antigen (ECA)","volume":"85","author":"Mayer H.","year":"1979","unstructured":"Mayer , H. , and G. Schmidt . 1979 . Chemistry and biology of the enterobacterial common antigen (ECA) . Curr. Top. Microbiol. Immunol. 85 : 99 - 153 .","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"p_24","unstructured":"Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/0022-2836(74)90036-9","article-title":"Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IV. The F sequences in F14","volume":"89","author":"Ohtsubo E.","year":"1974","unstructured":"Ohtsubo , E. , R. C. Deonier , H. J. Lee , and N. Davidson . 1974 . Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IV. The F sequences in F14 . J. Mol. Biol. 89 : 565 - 584 .","journal-title":"J. Mol. Biol."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1128\/jb.153.3.1172-1179.1983","article-title":"Identification of the uvrD gene product of Salmonella typhimurium LT2","volume":"153","author":"Pang P. P.","year":"1983","unstructured":"Pang , P. P. , and G. C. Walker . 1983 . Identification of the uvrD gene product of Salmonella typhimurium LT2 . J. Bacteriol. 153 : 1172 - 1179 .","journal-title":"J. Bacteriol."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1128\/jb.85.6.1402-1408.1963","article-title":"Gene transfer by F' strains of Escherichia coli K-12. II. Interaction between F-merogenote and chromosome during transfer","volume":"85","author":"Pittard J.","year":"1963","unstructured":"Pittard , J. , and E. A. Adelberg . 1963 . Gene transfer by F' strains of Escherichia coli K-12. II. Interaction between F-merogenote and chromosome during transfer . J. Bacteriol. 85 : 1402 - 1408 .","journal-title":"J. Bacteriol."},{"key":"p_28","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1099\/00207713-32-4-395","article-title":"Production of enterobacterial common antigen as an aid to classification of newly identified species of the families Enterobacteriaceae and","volume":"32","author":"Ramia S.","year":"1982","unstructured":"Ramia , S. , E. Neter , and D. J. Brenner . 1982 . Production of enterobacterial common antigen as an aid to classification of newly identified species of the families Enterobacteriaceae and Virbrionaceae. Int. J. Syst. Bacteriol. 32 : 395 - 398 .","journal-title":"Virbrionaceae. Int. J. Syst. Bacteriol."},{"key":"p_29","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1128\/jb.105.2.467-471.1971","article-title":"Mutant of Escherichia coli K-12 defective in D-glucosamine biosynthesis","volume":"105","author":"Sarvas M.","year":"1971","unstructured":"Sarvas , M. 1971 . Mutant of Escherichia coli K-12 defective in D-glucosamine biosynthesis . J. Bacteriol. 105 : 467 - 471 .","journal-title":"J. Bacteriol."},{"key":"p_30","first-page":"335","article-title":"Konjugation bei E. coli 08:K27(A):H-. Zentralbl. Bakteriol. 1 Abt","volume":"211","author":"Schmidt G.","year":"1969","unstructured":"Schmidt , G. 1969 . Konjugation bei E. coli 08:K27(A):H-. Zentralbl. Bakteriol. 1 Abt . Orig. A 211 : 335 - 344 .","journal-title":"Orig. A"},{"key":"p_31","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1128\/jb.127.2.755-762.1976","article-title":"Presence of rfe genes in Escherichia coli: their participation in biosynthesis of 0 antigen and enterobacterial common antigen","volume":"127","author":"Schmidt G.","year":"1976","unstructured":"Schmidt , G. , H. Mayer , and P. H. Mikel A. 1976 . Presence of rfe genes in Escherichia coli: their participation in biosynthesis of 0 antigen and enterobacterial common antigen . J. Bacteriol. 127 : 755 - 762 .","journal-title":"J. Bacteriol."},{"key":"p_32","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1128\/jb.98.3.1179-1194.1969","article-title":"Antipolarity in the ilv operon of Escherichia coli K-12","volume":"98","author":"Wechsler J. A.","year":"1969","unstructured":"Wechsler , J. A. , and E. A. Adeiberg . 1969 . Antipolarity in the ilv operon of Escherichia coli K-12 . J. Bacteriol. 98 : 1179 - 1194 .","journal-title":"J. Bacteriol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.163.2.756-762.1985","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.163.2.756-762.1985","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:30:02Z","timestamp":1627579802000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.163.2.756-762.1985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,8]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,8]]}},"alternative-id":["10.1128\/jb.163.2.756-762.1985"],"URL":"https:\/\/doi.org\/10.1128\/jb.163.2.756-762.1985","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,8]]},"assertion":[{"value":"2021-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}