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In order to explore the role of the prfA gene product in the pathogenesis of listerial infection, we constructed a site-directed insertion mutation in prfA by the chromosomal integration of a novel suicide vector containing a portion of the prfA coding region. This mutation not only transcriptionally silenced the listeriolysin (lisA) gene but also abrogated production of specific RNA transcripts corresponding to the phosphatidylinositol-specific phospholipase C (pic) and metalloprotease (mpl) genes, two further virulence gene products expressed only by pathogenic Listeria strains. The strain was also found to be avirulent when tested in a mouse model of listerial infection. The concomitant loss of multiple characteristics such as production of LisA, Pic, Mpl, and loss of virulence in a mouse infection model is the result of a mutation in a single gene and demonstrates that the prfA gene product is a positive regulator of multiple virulence determinants in L. monocytogenes.<\/jats:p>","DOI":"10.1128\/jb.174.2.568-574.1992","type":"journal-article","created":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T16:13:06Z","timestamp":1478275986000},"page":"568-574","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":353,"title":["Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene"],"prefix":"10.1128","volume":"174","author":[{"given":"T","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Medizinische Universit\u00e4ts Klinik, Klinische Biochemie, W\u00fcrzburg, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Leimeister-W\u00e4chter","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts Klinik, Klinische Biochemie, W\u00fcrzburg, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Domann","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts Klinik, Klinische Biochemie, W\u00fcrzburg, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Hartl","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts Klinik, Klinische Biochemie, W\u00fcrzburg, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Goebel","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts Klinik, Klinische Biochemie, W\u00fcrzburg, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Nichterlein","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts Klinik, Klinische Biochemie, W\u00fcrzburg, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Notermans","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts Klinik, Klinische Biochemie, W\u00fcrzburg, Federal Republic of Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"p_1","unstructured":"Ausubel F. 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