{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:17:46Z","timestamp":1791209866626,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1995,2,1]],"date-time":"1995-02-01T00:00:00Z","timestamp":791596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1995,2]]},"abstract":"<jats:p>A mycobacterial expression-secretion vector was constructed in which the Escherichia coli alkaline phosphatase (phoA) reporter gene was placed under the control of the Mycobacterium tuberculosis 85A promoter and secretion signal sequences. In recombinant Mycobacterium smegmatis and Mycobacterium bovis BCG, PhoA activity could readily be detected on the mycobacterial cell surface and in the culture supernatant, indicating that the 85A signals can drive heterologous expression and secretion in both species. In contrast to the mycobacteria, the 85A promoter did not function in E. coli. We mapped the promoter region by progressive deletions using BAL 31 exonuclease and by primer extension analysis. Insertion and deletion mutations within the promoter region indicated that, unlike most E. coli promoters but similar to Streptomyces promoters, the position of the putative -35 region was not critical for efficient promoter activity. In addition, we investigated the ability of the identified signals to drive the production and secretion in BCG of recombinant Schistosoma mansoni glutathione S-transferase (Sm28GST), a protective antigen against schistosomiasis. BALB\/c mice immunized with the recombinant BCG by a single dose exhibited a weak but specific T-cell response to Sm28GST.<\/jats:p>","DOI":"10.1128\/jb.177.3.642-653.1995","type":"journal-article","created":{"date-parts":[[2016,11,9]],"date-time":"2016-11-09T16:33:08Z","timestamp":1478709188000},"page":"642-653","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Analysis of the Mycobacterium tuberculosis 85A antigen promoter region"],"prefix":"10.1128","volume":"177","author":[{"given":"L","family":"Kremer","sequence":"first","affiliation":[{"name":"Laboratoire de Microbiologie G\u00e9n\u00e9tique et Mol\u00e9culaire INSERM CJF9109, Institut Pasteur, Lille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Baulard","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie G\u00e9n\u00e9tique et Mol\u00e9culaire INSERM CJF9109, Institut Pasteur, Lille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Estaquier","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie G\u00e9n\u00e9tique et Mol\u00e9culaire INSERM CJF9109, Institut Pasteur, Lille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Content","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie G\u00e9n\u00e9tique et Mol\u00e9culaire INSERM CJF9109, Institut Pasteur, Lille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Capron","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie G\u00e9n\u00e9tique et Mol\u00e9culaire INSERM CJF9109, Institut Pasteur, Lille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Locht","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie G\u00e9n\u00e9tique et Mol\u00e9culaire INSERM CJF9109, Institut Pasteur, Lille, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"3046","DOI":"10.1128\/IAI.56.12.3046-3051.1988","article-title":"Characterization of fibronectin-binding antigens by Mycobacterium tuberculosis and Mycobacterium bovis BCG","volume":"56","author":"Abou-Zeid C.","year":"1988","unstructured":"Abou-Zeid , C. , T. L. Ratliff , H. G. Wiker , M. Harboe , J. Bennedsen , and G. A. W. Rook . 1988 . Characterization of fibronectin-binding antigens by Mycobacterium tuberculosis and Mycobacterium bovis BCG . Infect. Immun. 56 : 3046 - 3051 .","journal-title":"Infect. Immun."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1038\/351479a0","article-title":"Humoral and cell-mediated immune responses to live recombinant BCG-HIV vaccines","volume":"351","author":"Aldovini A.","year":"1991","unstructured":"Aldovini , A. , and R. A. Young . 1991 . Humoral and cell-mediated immune responses to live recombinant BCG-HIV vaccines . Nature (London) 351 : 479 - 482 .","journal-title":"Nature (London)"},{"key":"p_3","doi-asserted-by":"crossref","first-page":"4105","DOI":"10.1093\/nar\/20.15.4105","article-title":"Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli","volume":"20","author":"Baulard A.","year":"1992","unstructured":"Baulard , A. , C. Jourdan , A. Mercenier , and C. Locht . 1992 . Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli . Nucleic Acids Res. 20 : 4105 .","journal-title":"Nucleic Acids Res."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1084\/jem.132.3.488","article-title":"Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs","volume":"132","author":"Boros D. L.","year":"1970","unstructured":"Boros , D. L. , and K. S. Warren . 1970 . Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs . J. Exp. Med. 132 : 488 - 507 .","journal-title":"J. Exp. Med."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1128\/IAI.57.10.3123-3130.1989","article-title":"Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis","volume":"57","author":"Borremans M.","year":"1989","unstructured":"Borremans , M. , L. DeWit , G. Volckaert , J. Ooms , J. De Bruyn , K. Huygen , J. P. Van Vooren , M. Stelandre , R. Verhofstadt , and J. Content . 1989 . Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis . Infect. Immun. 57 : 3123 - 3130 .","journal-title":"Infect. Immun."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1146\/annurev.me.43.020192.001233","article-title":"Immunologic aspects of schistosomiasis. Annu","volume":"43","author":"Capron A.","year":"1992","unstructured":"Capron , A. , and J. -P. Dessaint . 1992 . Immunologic aspects of schistosomiasis. Annu . Rev. Med. 43 : 209 - 218 .","journal-title":"Rev. Med."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/j.1365-2958.1994.tb00342.x","article-title":"Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria","volume":"11","author":"Cirillo J. D.","year":"1994","unstructured":"Cirillo , J. D. , T. R. Weisbrod , L. Pascopella , B. R. Bloom , and W. R. Jacobs , Jr. 1994 . Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria . Mol. Microbiol. 11 : 629 - 639 .","journal-title":"Mol. Microbiol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"11473","DOI":"10.1073\/pnas.90.24.11473","article-title":"Effective immunization against cutaneous leishmaniasis with recombinant bacille Calmette-Gu\u00e9rin expressing the Leishmania surface proteinase gp63","volume":"90","author":"Connell N. D.","year":"1993","unstructured":"Connell , N. D. , E. Medina-Acosta , W. R. McMaster , B. R. Bloom , and D. G. Russell . 1993 . Effective immunization against cutaneous leishmaniasis with recombinant bacille Calmette-Gu\u00e9rin expressing the Leishmania surface proteinase gp63 . Proc. Natl. Acad. Sci. USA 90 : 11473 - 11477 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_9","doi-asserted-by":"crossref","first-page":"3205","DOI":"10.1128\/IAI.59.9.3205-3212.1991","article-title":"The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis","volume":"59","author":"Content J.","year":"1991","unstructured":"Content , J. , A. de la Cuvellerie , L. De Wit , V. Vincent-Levy-Fr\u00e9bault , J. Ooms , and J. De Bruyn . 1991 . The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis . Infect. Immun. 59 : 3205 - 3212 .","journal-title":"Infect. Immun."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"5186","DOI":"10.1128\/JB.175.16.5186-5192.1993","article-title":"Cloning and assessment of mycobacterial promoters by using a plasmid shuttle vector","volume":"175","author":"Das Gupta S. K.","year":"1993","unstructured":"Das Gupta , S. K. , M. D. Bashyam , and A. K. Tyagi . 1993 . Cloning and assessment of mycobacterial promoters by using a plasmid shuttle vector . J. Bacteriol. 175 : 5186 - 5192 .","journal-title":"J. Bacteriol."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1111\/j.1365-2958.1993.tb00970.x","article-title":"Construction and use of integrative vectors to express foreign genes in mycobacteria","volume":"10","author":"Dellagostin O. A.","year":"1993","unstructured":"Dellagostin , O. A. , S. Wall , E. Norman , T. O'Shaughnessy , J. W. Dale , and J. McFadden . 1993 . Construction and use of integrative vectors to express foreign genes in mycobacteria . Mol. Microbiol. 10 : 983 - 993 .","journal-title":"Mol. Microbiol."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.1093\/nar\/18.13.3995","article-title":"Nucleotide sequence of the 32 kDa-protein gene (antigen 85A) of Mycobacterium bovis BCG","volume":"18","author":"De Wit L.","year":"1990","unstructured":"De Wit , L. , A. De la Cuvellerie , J. Ooms , and J. Content . 1990 . Nucleotide sequence of the 32 kDa-protein gene (antigen 85A) of Mycobacterium bovis BCG . Nucleic Acids Res. 18 : 3995 .","journal-title":"Nucleic Acids Res."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0166-6851(93)90249-W","article-title":"Stable integration and expression of the Plasmodium falciparum circumsporozoite protein coding sequence in mycobacteria","volume":"57","author":"Haeseleer F.","year":"1993","unstructured":"Haeseleer , F. , J. F. Pollet , M. Haumont , A. Bollen , and P. Jacobs . 1993 . Stable integration and expression of the Plasmodium falciparum circumsporozoite protein coding sequence in mycobacteria . Mol. Biochem. Parasitol. 57 : 117 - 126 .","journal-title":"Mol. Biochem. Parasitol."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1365-3083.1988.tb02338.x","article-title":"Specific Iymphoproliferation, gamma interferon production, and serum immunoglobulin G directed against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis","volume":"27","author":"Huygen K.","year":"1988","unstructured":"Huygen , K. , J. P. van Vooren , M. Turneer , R. Bosmans , P. Dierckx , and J. De Bruyn . 1988 . Specific Iymphoproliferation, gamma interferon production, and serum immunoglobulin G directed against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis . Scand. J. Immunol. 27 : 187 - 194 .","journal-title":"Scand. J. Immunol."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/BF00259397","article-title":"Tandem promoters, tsrp1 and tsrp2, direct transcription of the thiostrepton resistance gene (tsr) of Streptomyces azureus: transcriptional initiation from tsrp2 occurs after deletion of the ~35 region","volume":"221","author":"Janssen G. R.","year":"1990","unstructured":"Janssen , G. R. , and M. J. Bibb . 1990 . Tandem promoters, tsrp1 and tsrp2, direct transcription of the thiostrepton resistance gene (tsr) of Streptomyces azureus: transcriptional initiation from tsrp2 occurs after deletion of the ~35 region . Mol. Gen. Genet. 221 : 339 - 346 .","journal-title":"Mol. Gen. Genet."},{"key":"p_16","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1099\/13500872-140-1-123","article-title":"Nucleotide sequence and secondary structures of precursor 16S rRNA of slow-growing mycobacteria","volume":"140","author":"Ji Y.","year":"1994","unstructured":"Ji , Y. , M. J. Colston , and R. A. Cox . 1994 . Nucleotide sequence and secondary structures of precursor 16S rRNA of slow-growing mycobacteria . Microbiology 140 : 123 - 132 .","journal-title":"Microbiology"},{"key":"p_17","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0264-410X(94)90054-X","article-title":"Cytotoxic T Iymphocyte response in mice induced by recombinant BCG vaccination which produces an extracellular ~ antigen that fused with the human immunodeficiency virus type 1 envelope immunodominant domain in the V3 loop","volume":"12","author":"Kameoka M.","year":"1994","unstructured":"Kameoka , M. , Y. Nishino , K. Matsuo , N. Ohara , T. Kimura , A. Yamazaki , T. Yamada , and K. Ikuta . 1994 . Cytotoxic T Iymphocyte response in mice induced by recombinant BCG vaccination which produces an extracellular ~ antigen that fused with the human immunodeficiency virus type 1 envelope immunodominant domain in the V3 loop . Vaccine 12 : 153 - 158 .","journal-title":"Vaccine"},{"key":"p_18","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1128\/JB.168.1.72-80.1986","article-title":"Cloning and expression of Mycobacterium bovis BCG DNA in Streptomyces lividans","volume":"168","author":"Kieser T.","year":"1986","unstructured":"Kieser , T. , M. T. Moss , J. W. Dale , and D. A. Hopwood . 1986 . Cloning and expression of Mycobacterium bovis BCG DNA in Streptomyces lividans . J. Bacteriol. 168 : 72 - 80 .","journal-title":"J. Bacteriol."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1093\/nar\/18.4.1069","article-title":"Engineered gene for Escherichia coli alkaline phosphatase for the construction of translational fusions","volume":"18","author":"Kohl J.","year":"1990","unstructured":"Kohl , J. , F. R\u00fcker , G. Himmler , D. Mattanovich , and H. Katinger . 1990 . Engineered gene for Escherichia coli alkaline phosphatase for the construction of translational fusions . Nucleic Acids Res. 18 : 1069 .","journal-title":"Nucleic Acids Res."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1073\/pnas.90.7.2608","article-title":"Mycobacterium tuberculosis expresses two chaperonin-60 homologs","volume":"90","author":"Kong T. H.","year":"1993","unstructured":"Kong , T. H. , A. R. M. Coates , P. D. Butcher , C. J. Hickman , and T. M. Shinnick . 1993 . Mycobacterium tuberculosis expresses two chaperonin-60 homologs . Proc. Natl. Acad. Sci. USA 90 : 2608 - 2612 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_21","unstructured":".Kremer L. Unpublished data."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0769-2609(85)80045-4","article-title":"Restriction endonuclease mapping and cloning of Mycobacterium fortuitum var. fortuitum plasmid pAL5000","volume":"136","author":"Labidi A.","year":"1985","unstructured":"Labidi , A. , H. L. David , and D. Roulland-Dussoix . 1985 . Restriction endonuclease mapping and cloning of Mycobacterium fortuitum var. fortuitum plasmid pAL5000 . Ann. Inst. Pasteur Microbiol. 136 : 209 - 215 .","journal-title":"Ann. Inst. Pasteur Microbiol."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli U. K.","year":"1970","unstructured":"Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 .","journal-title":"Nature (London)"},{"key":"p_24","doi-asserted-by":"crossref","first-page":"8129","DOI":"10.1073\/pnas.82.23.8129","article-title":"TnphoA: a transposon probe for protein export signals","volume":"82","author":"Manoil C.","year":"1985","unstructured":"Manoil , C. , and J. Beckwith . 1985 . TnphoA: a transposon probe for protein export signals . Proc. Natl. Acad. Sci. USA 82 : 8129 - 8133 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_25","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1128\/IAI.58.2.550-556.1990","article-title":"Cloning and expression of the gene for the cross-reactive ~ antigen of Mycobacterium kansasii","volume":"58","author":"Matsuo K.","year":"1990","unstructured":"Matsuo , K. , R. Yamaguchi , A. Yamazaki , H. Tasaka , K. Terasaka , and T. Yamada . 1990 . Cloning and expression of the gene for the cross-reactive ~ antigen of Mycobacterium kansasii . Infect. Immun. 58 : 550 - 556 .","journal-title":"Infect. Immun."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"4049","DOI":"10.1128\/IAI.58.12.4049-4054.1990","article-title":"Establishment of a foreign antigen secretion system in mycobacteria","volume":"58","author":"Matsuo K.","year":"1990","unstructured":"Matsuo , K. , R. Yamaguchi , A. Yamazaki , H. Tasaka , K. Terasaka , M. Totsuka , K. Kobayashi , H. Yukitake , and T. Yamada . 1990 . Establishment of a foreign antigen secretion system in mycobacteria . Infect. Immun. 58 : 4049 - 4054 .","journal-title":"Infect. Immun."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JB.174.1.1-7.1992","article-title":"Environmental signals controlling expression of virulence determinants in bacteria","volume":"174","author":"Mekalanos J. J.","year":"1992","unstructured":"Mekalanos , J. J. 1992 . Environmental signals controlling expression of virulence determinants in bacteria . J. Bacteriol. 174 : 1 - 7 .","journal-title":"J. Bacteriol."},{"key":"p_28","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1021\/ja00897a025","article-title":"Solid phase peptide synthesis","volume":"85","author":"Merrifield R. B.","year":"1963","unstructured":"Merrifield , R. B. 1963 . Solid phase peptide synthesis . J. Am. Chem. Soc. 85 : 2149 - 2154 .","journal-title":"J. Am. Chem. Soc."},{"key":"p_29","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.1111\/j.1365-2958.1992.tb02201.x","article-title":"Expression of Escherichia coli ~-galactosidase in Mycobacterium bovis BCG using an expression system isolated from Mycobacterium paratuberculosis which induced humoral and cellular immune responses","volume":"6","author":"Murray A.","year":"1992","unstructured":"Murray , A. , N. Winter , M. Lagranderie , D. F. Hill , J. Rauzier , J. Timm , C. Leclerc , K. M. Moriarty , M. Gheorghiu , and B. Gicquel . 1992 . Expression of Escherichia coli ~-galactosidase in Mycobacterium bovis BCG using an expression system isolated from Mycobacterium paratuberculosis which induced humoral and cellular immune responses . Mol. Microbiol. 6 : 3331 - 3342 .","journal-title":"Mol. Microbiol."},{"key":"p_30","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1128\/IAI.59.1.372-382.1991","article-title":"Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis","volume":"59","author":"Nagai S.","year":"1991","unstructured":"Nagai , S. , H. G. Wiker , M. Harboe , and M. Kinomoto . 1991 . Isolation and partial characterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis . Infect. Immun. 59 : 372 - 382 .","journal-title":"Infect. Immun."},{"key":"p_31","doi-asserted-by":"crossref","first-page":"4452","DOI":"10.1128\/IAI.60.11.4452-4459.1992","article-title":"Mycobacterium leprae produces extracellular homologs of the antigen 85 complex","volume":"60","author":"Pessolani M. C. V.","year":"1992","unstructured":"Pessolani , M. C. V. , and P. J. Brennan . 1992 . Mycobacterium leprae produces extracellular homologs of the antigen 85 complex . Infect. Immun. 60 : 4452 - 4459 .","journal-title":"Infect. Immun."},{"key":"p_32","unstructured":".Pierce R. et al. Submitted for publication."},{"key":"p_33","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0378-1119(84)90059-3","article-title":"In vitro insertional mutagenesis with a selectable DNA fragment","volume":"29","author":"Prentki P.","year":"1984","unstructured":"Prentki , P. , and H. Krisch . 1984 . In vitro insertional mutagenesis with a selectable DNA fragment . Gene 29 : 303 - 313 .","journal-title":"Gene"},{"key":"p_34","unstructured":"Radford A. J. and P. R. Wood. 1990. Expression system for actinomycetes and related organisms. International patent application WO90\/10701."},{"key":"p_35","doi-asserted-by":"crossref","first-page":"2793","DOI":"10.1128\/JB.172.5.2793-2797.1990","article-title":"Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a ``mini'' Mycobacterium-Escherichia coli shuttle vector","volume":"172","author":"Ranes M. G.","year":"1990","unstructured":"Ranes , M. G. , J. Rauzier , M. Lagranderie , M. Gheorghiu , and B. Gicquel . 1990 . Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a ``mini'' Mycobacterium-Escherichia coli shuttle vector . J. Bacteriol. 172 : 2793 - 2797 .","journal-title":"J. Bacteriol."},{"key":"p_36","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1128\/IAI.61.9.3642-3647.1993","article-title":"The Mycobacterium leprae antigen 85 complex gene family: identification of the genes for the 85A, 85C, and related MPT51 proteins","volume":"61","author":"Rinke-De Wit T. F.","year":"1993","unstructured":"Rinke-De Wit , T. F. , S. Bekelie , A. Osland , B. Wieles , A. A. M. Janson , and J. E. R. Thole . 1993 . The Mycobacterium leprae antigen 85 complex gene family: identification of the genes for the 85A, 85C, and related MPT51 proteins . Infect. Immun. 61 : 3642 - 3647 .","journal-title":"Infect. Immun."},{"key":"p_37","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"p_38","first-page":"860","article-title":"Sur la nutrition min\u00e9rale du bacille tuberculeux","volume":"155","author":"Sauton B.","year":"1912","unstructured":"Sauton , B. 1912 . Sur la nutrition min\u00e9rale du bacille tuberculeux . C. R. Acad. Sci. Paris 155 : 860 .","journal-title":"C. R. Acad. Sci. Paris"},{"key":"p_39","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0378-1119(91)90114-Q","article-title":"Cloning and characterization of the Mycobacterium leprae ribosomal RNA promoter in Escherichia coli","volume":"98","author":"Sela S.","year":"1991","unstructured":"Sela , S. , and J. Clark-Curtiss . 1991 . Cloning and characterization of the Mycobacterium leprae ribosomal RNA promoter in Escherichia coli . Gene 98 : 123 - 127 .","journal-title":"Gene"},{"key":"p_40","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1574-6968.1989.tb03100.x","article-title":"Molecular cloning of mycobacterial promoters in E. coli","volume":"59","author":"Sirokova T. D.","year":"1989","unstructured":"Sirokova , T. D. , S. S. Bardarov , J. I. Kriakov , and K. I. Markov . 1989 . Molecular cloning of mycobacterial promoters in E. coli . FEMS Microbiol. Lett. 59 : 153 - 156 .","journal-title":"FEMS Microbiol. Lett."},{"key":"p_41","doi-asserted-by":"crossref","first-page":"6987","DOI":"10.1073\/pnas.85.18.6987","article-title":"Lysogeny and transformation in mycobacteria: stable expression of foreign genes","volume":"85","author":"Snapper S. B.","year":"1988","unstructured":"Snapper , S. B. , L. Lugosi , A. Jekkel , R. E. Melton , T. Kieser , B. R. Bloom , and W. R. Jacobs , Jr. 1988 . Lysogeny and transformation in mycobacteria: stable expression of foreign genes . Proc. Natl. Acad. Sci. USA 85 : 6987 - 6991 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_42","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1111\/j.1365-2958.1990.tb02040.x","article-title":"Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis","volume":"4","author":"Snapper S. B.","year":"1990","unstructured":"Snapper , S. B. , R. E. Melton , S. Mustafa , T. Kieser , and W. R. Jacobs , Jr. 1990 . Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis . Mol. Microbiol. 4 : 1911 - 1919 .","journal-title":"Mol. Microbiol."},{"key":"p_43","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1084\/jem.178.1.197","article-title":"Protective immunity elicited by recombinant bacille-Calmette-Gu\u00e9rin (BCG) expressing outer surface protein A (Osp A) lipoprotein: a candidate lyme disease vaccine","volume":"178","author":"Stover K.","year":"1993","unstructured":"Stover , K. , G. P. Bansal , M. S. Hanson , J. E. Burlein , S. R. Palaszinski , J. F. Young , S. Koenig , D. B. Young , A. Sadziene , and A. G. Barbour . 1993 . Protective immunity elicited by recombinant bacille-Calmette-Gu\u00e9rin (BCG) expressing outer surface protein A (Osp A) lipoprotein: a candidate lyme disease vaccine . J. Exp. Med. 178 : 197 - 209 .","journal-title":"J. Exp. Med."},{"key":"p_44","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1038\/351456a0","article-title":"New use of BCG for recombinant vaccines","volume":"351","author":"Stover C. K.","year":"1991","unstructured":"Stover , C. K. , V. F. de la Cruz , T. R. Fuerst , J. E. Burlein , L. A. Benson , L. T. Bennett , G. P. Bansal , J. F. Young , M. H. Lee , G. F. Hatfull , S. B. Snapper , R. G. Barletta , W. R. Jacobs , Jr. , and B. R. Bloom . 1991 . New use of BCG for recombinant vaccines . Nature (London) 351 : 456 - 460 .","journal-title":"Nature (London)"},{"key":"p_45","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1093\/nar\/20.5.961","article-title":"Compilation and analysis of DNA sequences associated with apparent streptomycete promoters","volume":"20","author":"Strohl W. R.","year":"1992","unstructured":"Strohl , W. R. 1992 . Compilation and analysis of DNA sequences associated with apparent streptomycete promoters . Nucleic Acids Res. 20 : 961 - 974 .","journal-title":"Nucleic Acids Res."},{"key":"p_46","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1073\/pnas.82.4.1074","article-title":"A bacteriophage T7 RNA polymerase\/promoter system for controlled exclusive expression of specific genes","volume":"82","author":"Tabor S.","year":"1985","unstructured":"Tabor , S. , and C. C. Richardson . 1985 . A bacteriophage T7 RNA polymerase\/promoter system for controlled exclusive expression of specific genes . Proc. Natl. Acad. Sci. USA 82 : 1074 - 1078 .","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"p_47","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1574-6968.1989.tb03058.x","article-title":"Complete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG","volume":"58","author":"Terasaka K.","year":"1989","unstructured":"Terasaka , K. , R. Yamaguchi , K. Matsuo , A. Yamazaki , S. Nagai , and T. Yamada . 1989 . Complete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG . FEMS Microbiol. Lett. 58 : 273 - 276 .","journal-title":"FEMS Microbiol. Lett."},{"key":"p_48","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1128\/IAI.55.6.1466-1475.1987","article-title":"Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in Escherichia coli","volume":"55","author":"Thole J. E. R.","year":"1987","unstructured":"Thole , J. E. R. , W. J. Keulen , A. H. J. Kolk , D. G. Groothuis , L. G. Berwald , R. H. Tiesjema , and J. D. A. van Embden . 1987 . Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in Escherichia coli . Infect. Immun. 55 : 1466 - 1475 .","journal-title":"Infect. Immun."},{"key":"p_49","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/j.1365-2958.1994.tb01037.x","article-title":"Transcription and expression analysis using lacZ and phoA gene fusions, of Mycobacterium fortuitum ~-lactamase genes cloned from a natural isolate and a high-level ~-lactamase producer","volume":"12","author":"Timm J.","year":"1994","unstructured":"Timm , J. , M. G. Perilli , C. Duez , J. Trias , G. Orefici , L. Fattorini , G. Amicosante , A. Oratore , B. Joris , J. M. Fr\u00e8re , A. P. Pugsley , and B. Gicquel . 1994 . Transcription and expression analysis using lacZ and phoA gene fusions, of Mycobacterium fortuitum ~-lactamase genes cloned from a natural isolate and a high-level ~-lactamase producer . Mol. Microbiol. 12 : 491 - 504 .","journal-title":"Mol. Microbiol."},{"key":"p_50","unstructured":"Torriani A. 1967. Alkaline phosphatase from Escherichia coli p. 224-235. In G. L. Cantoni and D. R. Davies (ed.) Procedures in nucleic acid research. Harper & Row Publishers Inc. New York."},{"key":"p_51","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1002\/eji.1830210804","article-title":"A monoclonal antibody blocking the Schistosoma mansoni 28-kDa glutathione S-transferase activity reduces female worm fecundity and egg viability","volume":"21","author":"Xu C.","year":"1991","unstructured":"Xu , C. - B. , C. Verwaerde , J. -M. Grzych , J. Fontaine , and A. Capron . 1991 . A monoclonal antibody blocking the Schistosoma mansoni 28-kDa glutathione S-transferase activity reduces female worm fecundity and egg viability . Eur. J. Immunol. 21 : 1801 - 1807 .","journal-title":"Eur. J. Immunol."},{"key":"p_52","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1128\/MR.56.4.648-661.1992","article-title":"The antigen 85 complex: a major secretion product of Mycobacterium tuberculosis","volume":"56","author":"Wiker H. G.","year":"1992","unstructured":"Wiker , H. G. , and M. Harboe . 1992 . The antigen 85 complex: a major secretion product of Mycobacterium tuberculosis . Microbiol. Rev. 56 : 648 - 661 .","journal-title":"Microbiol. Rev."},{"key":"p_53","unstructured":"World Health Organization. 1991. Expanded programme on immunization update. World Health Organization Geneva."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.177.3.642-653.1995","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.177.3.642-653.1995","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:53:45Z","timestamp":1627581225000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.177.3.642-653.1995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,2]]},"references-count":53,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,2]]}},"alternative-id":["10.1128\/jb.177.3.642-653.1995"],"URL":"https:\/\/doi.org\/10.1128\/jb.177.3.642-653.1995","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,2]]},"assertion":[{"value":"2021-01-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}