{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:55:40Z","timestamp":1791348940023,"version":"4.3.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genome of\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            , the causative agent of plague, contains at least 30 copies of an element, designated IS\n            <jats:italic>1541<\/jats:italic>\n            , which is structurally related to IS\n            <jats:italic>200<\/jats:italic>\n            (85% identity). One such element is inserted within the chromosomal\n            <jats:italic>inv<\/jats:italic>\n            gene (M. Simonet, B. Riot, N. Fortineau, and P. Berche, Infect. Immun. 64:375\u2013379, 1996). We characterized other IS\n            <jats:italic>1541<\/jats:italic>\n            insertions by cloning 14 different\n            <jats:italic>Y. pestis<\/jats:italic>\n            6\/69M loci carrying a single copy of this insertion sequence (IS) into\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and, for each element, sequencing 250 bp of both flanking regions. In no case was this IS element inserted into large open reading frames; however, in eight cases, it was detected downstream (17 to 139 bp) of genes thought to be transcribed monocistronically or which constituted the last gene of an operon, and in only one case was it detected upstream (37 bp) of the first gene of an operon. Sequence analysis revealed stem-loop structures (\u0394\n            <jats:italic>G<\/jats:italic>\n            , &lt;\u221210 kcal) resembling rho-independent transcription terminators in 8 of the 14 insertion sites. These motifs might constitute hot spots for insertion of this IS\n            <jats:italic>1541<\/jats:italic>\n            element within the\n            <jats:italic>Y. pestis<\/jats:italic>\n            genome.\n          <\/jats:p>","DOI":"10.1128\/jb.180.1.178-181.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T16:22:57Z","timestamp":1577809377000},"page":"178-181","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Molecular Characterization of IS\n            <i>1541<\/i>\n            Insertions in the Genome of\n            <i>Yersinia pestis<\/i>"],"prefix":"10.1128","volume":"180","author":[{"given":"Monique","family":"Odaert","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Faculte\u0301 de Me\u0301decine Henri Warembourg, Lille,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Devalckenaere","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Faculte\u0301 de Me\u0301decine Henri Warembourg, Lille,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Trieu-Cuot","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->INSERM U411, Faculte\u0301 de Me\u0301decine Necker, Paris,2 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Simonet","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Faculte\u0301 de Me\u0301decine Henri Warembourg, Lille,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1093\/genetics\/133.3.449","article-title":"Natural populations of Escherichia coli and Salmonella typhimurium harbor the same classes of insertion sequences","volume":"133","author":"Biser\u010di\u0107 M.","year":"1993","unstructured":"Biser\u010di\u0107 M. Ochman H. Natural populations of Escherichia coli and Salmonella typhimurium harbor the same classes of insertion sequences.Genetics1331993449454","journal-title":"Genetics"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"10610","DOI":"10.1016\/S0021-9258(18)67429-0","article-title":"Glutamyl-tRNA synthetase of Escherichia coli. Isolation and primary structure of the gltX gene and homology with other aminoacyl-tRNA synthetases","volume":"261","author":"Breton R.","year":"1986","unstructured":"Breton R. Sanfa\u00e7on H. Papayannopoulos I. Biemann K. Lapointe J. Glutamyl-tRNA synthetase of Escherichia coli. Isolation and primary structure of the gltX gene and homology with other aminoacyl-tRNA synthetases.J. Biol. Chem.26119861061010617","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.3.309"},{"key":"e_1_3_2_5_2","unstructured":"Carniel E. 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