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We found that deletion of\n            <jats:italic>SOD1<\/jats:italic>\n            (encoding Cu\/Zn superoxide dismutase) rendered\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            hypersensitive to oxytetracycline (OTC): a\n            <jats:italic>sod1\u0394<\/jats:italic>\n            mutant exhibited a &gt;95% reduction in colony-forming ability at an OTC concentration of 20 \u03bcg ml\n            <jats:sup>\u22121<\/jats:sup>\n            , whereas concentrations of up to 1,000 \u03bcg ml\n            <jats:sup>\u22121<\/jats:sup>\n            had no effect on the growth of the wild type. OTC resistance was restored in the\n            <jats:italic>sod1<\/jats:italic>\n            \u0394 mutant by complementation with wild-type\n            <jats:italic>SOD1<\/jats:italic>\n            . The effect of OTC appeared to be cytotoxic and was not evident in a\n            <jats:italic>ctt1<\/jats:italic>\n            \u0394 (cytosolic catalase) mutant or in the presence of tetracycline.\n            <jats:italic>SOD1<\/jats:italic>\n            transcription was not induced by OTC, suggesting that constitutive\n            <jats:italic>SOD1<\/jats:italic>\n            expression is sufficient for wild-type OTC resistance. OTC uptake levels in wild-type and\n            <jats:italic>sod1<\/jats:italic>\n            \u0394 strains were similar. However, lipid peroxidation and protein oxidation were both enhanced during exposure of the\n            <jats:italic>sod1<\/jats:italic>\n            \u0394 mutant, but not the wild type, to OTC. We propose that Sod1p protects\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            against a mode of OTC action that is dependent on oxidative damage.\n          <\/jats:p>","DOI":"10.1128\/jb.182.1.76-80.2000","type":"journal-article","created":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T18:36:02Z","timestamp":1240511762000},"page":"76-80","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Copper\/Zinc-Superoxide Dismutase Is Required for Oxytetracycline Resistance of\n            <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1128","volume":"182","author":[{"given":"Simon V.","family":"Avery","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Georgia State University, Atlanta, Georgia 30303"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srividya","family":"Malkapuram","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Georgia State University, Atlanta, Georgia 30303"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Mateus","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Georgia State University, Atlanta, Georgia 30303"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly S.","family":"Babb","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Georgia State University, Atlanta, Georgia 30303"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"390","article-title":"Adverse reaction to oxytetracycline in a dog","volume":"27","author":"Abdullahi S. 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