{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T21:31:36Z","timestamp":1793136696626,"version":"4.5.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2000,6,15]],"date-time":"2000-06-15T00:00:00Z","timestamp":961027200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , the MarA protein controls expression of multiple chromosomal genes affecting resistance to antibiotics and other environmental hazards. For a more-complete characterization of the\n            <jats:italic>mar<\/jats:italic>\n            regulon, duplicate macroarrays containing 4,290 open reading frames of the\n            <jats:italic>E. coli<\/jats:italic>\n            genome were hybridized to radiolabeled cDNA populations derived from\n            <jats:italic>mar<\/jats:italic>\n            -deleted and\n            <jats:italic>mar<\/jats:italic>\n            -expressing\n            <jats:italic>E. coli<\/jats:italic>\n            . Strains constitutively expressing MarA showed altered expression of more than 60 chromosomal genes: 76% showed increased expression and 24% showed decreased expression. Although some of the genes were already known to be MarA regulated, the majority were newly determined and belonged to a variety of functional groups. Some of the genes identified have been associated with iron transport and metabolism; other genes were previously known to be part of the\n            <jats:italic>soxRS<\/jats:italic>\n            regulon. Northern blot analysis of selected genes confirmed the results obtained with the macroarrays. The findings reveal that the\n            <jats:italic>mar<\/jats:italic>\n            locus mediates a global stress response involving one of the largest networks of genes described.\n          <\/jats:p>","DOI":"10.1128\/jb.182.12.3467-3474.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"3467-3474","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":329,"title":["Differential Expression of over 60 Chromosomal Genes in\n            <i>Escherichia coli<\/i>\n            by Constitutive Expression of MarA"],"prefix":"10.1128","volume":"182","author":[{"given":"Teresa M.","family":"Barbosa","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Center for Adaptation Genetics and Drug Resistance and the Departments of Molecular Biology and Microbiology1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart B.","family":"Levy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Center for Adaptation Genetics and Drug Resistance and the Departments of Molecular Biology and Microbiology1 and"},{"name":"<!--label omitted: 2-->Medicine,2 Tufts University School of Medicine, Boston, Massachusetts 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.15.4669-4672.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2067"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.4.938-944.1998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.1.143-148.1994"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.7.1655-1661.1995"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.4.1428-1433.1997"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S1367-5931(99)80027-1","article-title":"Fe-S proteins in sensing and regulatory functions","volume":"3","author":"Beinert H.","year":"1999","unstructured":"Beinert H. Kiley P. J. Fe-S proteins in sensing and regulatory functions.Curr. Opin. Chem. Biol.31999152157","journal-title":"Curr. Opin. Chem. Biol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5331.1453"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S1369-5274(99)80021-6","article-title":"Cell regulation. Maintaining integrity and efficiency in microbial cells","volume":"2","author":"Chater K. F.","year":"1999","unstructured":"Chater K. F. Nikaido H. Cell regulation. Maintaining integrity and efficiency in microbial cells.Curr. Opin. Microbiol.21999121125","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"8950","DOI":"10.1073\/pnas.92.19.8950","article-title":"A general genetic approach in Escherichia coli for determining the mechanism(s) of action of tumoricidal agents: application to DMP 840, a tumoricidal agent","volume":"92","author":"Chatterjee P. K.","year":"1995","unstructured":"Chatterjee P. K. Sternberg N. L. A general genetic approach in Escherichia coli for determining the mechanism(s) of action of tumoricidal agents: application to DMP 840, a tumoricidal agent.Proc. Natl. Acad. Sci. USA92199589508954","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.5.1484-1492.1993"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.24.7856-7862.1993"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.12.5416-5422.1988"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1093\/infdis\/168.2.484","article-title":"A multidrug resistance regulatory chromosomal locus is widespread among enteric bacteria","volume":"168","author":"Cohen S. P.","year":"1993","unstructured":"Cohen S. P. Yan W. Levy S. B. A multidrug resistance regulatory chromosomal locus is widespread among enteric bacteria.J. Infect. Dis.1681993484488","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"3795","DOI":"10.1099\/00221287-143-12-3795","article-title":"Transcriptional regulation of the aconitase genes (acnA and acnB) of Escherichia coli","volume":"143","author":"Cunningham L.","year":"1997","unstructured":"Cunningham L. Gruer M. J. Guest J. R. Transcriptional regulation of the aconitase genes (acnA and acnB) of Escherichia coli.Microbiology143199737953805","journal-title":"Microbiology"},{"key":"e_1_3_2_17_2","first-page":"33","article-title":"Antisense micF RNA and 5\u2032-UTR of the target ompF RNA: phylogenetic conservation of primary and secondary structures","volume":"36","author":"Delihas N.","year":"1997","unstructured":"Delihas N. Antisense micF RNA and 5\u2032-UTR of the target ompF RNA: phylogenetic conservation of primary and secondary structures.Nucleic Acids Symp. Ser.3619973335","journal-title":"Nucleic Acids Symp. Ser."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0378-1119(96)00329-0","article-title":"Redox signaling and gene control in the Escherichia coli soxRS oxidative stress regulon\u2014a review","volume":"179","author":"Demple B.","year":"1996","unstructured":"Demple B. Redox signaling and gene control in the Escherichia coli soxRS oxidative stress regulon\u2014a review.Gene17919965357","journal-title":"Gene"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/S0966-842X(99)01510-3","article-title":"Protective mechanisms against toxic electrophiles in Escherichia coli","volume":"7","author":"Ferguson G. P.","year":"1999","unstructured":"Ferguson G. P. Protective mechanisms against toxic electrophiles in Escherichia coli.Trends Microbiol.71999242247","journal-title":"Trends Microbiol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.19.5803-5805.1996"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.4.393-410.1997"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.155.2.531-540.1983"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.155.2.541-548.1983"},{"key":"e_1_3_2_24_2","unstructured":"Gralla\nJ. D.\nCollado-Vides\nJ.\nOrganization and function of transcriptional regulatory elements\nEscherichia coli and Salmonella: cellular and molecular biology\n2nd ed.\nNeidhardt\nF. C.\nCurtiss\nR.\nIII\nIngraham\nJ. L.\nLin\nE. C. C.\nLow\nK. B.\nMagasanik\nB.\nReznikoff\nW. S.\nRiley\nM.\nSchaechter\nM.\nUmbarger\nH. E.\n1996\n1232\n1245\nAmerican Society for Microbiology\nWashington D.C."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1099\/00221287-143-9-2865","article-title":"Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr","volume":"143","author":"Green J.","year":"1997","unstructured":"Green J. Anjum M. F. Guest J. R. Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr.Microbiology143199728652875","journal-title":"Microbiology"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.14.4433-4439.1991"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.9.4617-4622.1989"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","unstructured":"Hidalgo\nE.\nDemple\nB.\nAdaptive response to oxidative stress: the soxRS and oxyR regulons\nRegulation of gene expression in Escherichia coli. R. G.\nLin\nE. C. C.\nLynch\nA. S.\n1995\n433\n450\nLandes Company\nAustin Tex","DOI":"10.1007\/978-1-4684-8601-8_21"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/BF00267469","article-title":"Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12","volume":"229","author":"Hogg R. W.","year":"1991","unstructured":"Hogg R. W. Voelker C. von Carlowitz I. Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12.Mol. Gen. Genet.2291991453459","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.9.2507-2513.1996"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.24.7100-7104.1995"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1007\/s004380050978","article-title":"The reversed SoxS-binding site upstream of the ribA promoter in Escherichia coli","volume":"261","author":"Koh Y. S.","year":"1999","unstructured":"Koh Y. S. Chung W.-H. Lee J.-H. Roe J.-H. The reversed SoxS-binding site upstream of the ribA promoter in Escherichia coli.Mol. Gen. Genet.2611999374380","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1099\/00221287-143-6-2085","article-title":"The aldA gene of Escherichia coli is under the control of at least three transcriptional regulators","volume":"143","author":"Limon A.","year":"1997","unstructured":"Limon A. Hidalgo E. Aguilar J. The aldA gene of Escherichia coli is under the control of at least three transcriptional regulators.Microbiology143199720852095","journal-title":"Microbiology"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1073\/pnas.96.7.3537","article-title":"Nitroreductase A is regulated as a member of the soxRS regulon of Escherichia coli","volume":"96","author":"Liochev S. I.","year":"1999","unstructured":"Liochev S. I. Hausladen A. Fridovich I. Nitroreductase A is regulated as a member of the soxRS regulon of Escherichia coli.Proc. Natl. Acad. Sci. USA96199935373539","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.137"},{"key":"e_1_3_2_36_2","unstructured":"Lynch\nA. S.\nLin\nE. C. C.\nResponses to molecular oxygen\nEscherichia coli and Salmonella: cellular and molecular biology\n2nd ed.\nNeidhardt\nF. C.\nCurtiss\nR.\nIII\nIngraham\nJ. L.\nLin\nE. C. C.\nLow\nK. B.\nMagasanik\nB.\nReznikoff\nW. S.\nRiley\nM.\nSchaechter\nM.\nUmbarger\nH. E.\n1996\n1526\n1538\nAmerican Society for Microbiology\nWashington D.C."},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1128\/AAC.40.7.1695","article-title":"Identification of mar mutants among quinolone-resistant clinical isolates of Escherichia coli","volume":"40","author":"Manneewannakul K.","year":"1996","unstructured":"Manneewannakul K. Levy S. B. Identification of mar mutants among quinolone-resistant clinical isolates of Escherichia coli.Antimicrob. Agents Chemother.40199616951698","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1046\/j.1365-2958.1999.01599.x","article-title":"Structural requirements for marbox function in transcriptional activation of mar\/sox\/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter","volume":"34","author":"Martin R. G.","year":"1999","unstructured":"Martin R. G. Gillette W. K. Rhee S. Rosner J. L. Structural requirements for marbox function in transcriptional activation of mar\/sox\/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter.Mol. Microbiol.341999431441","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1046\/j.1365-2958.2000.01732.x","article-title":"Promoter discrimination by the related transcriptional activators MarA and SoxS: differential regulation by differential binding","volume":"35","author":"Martin R. G.","year":"2000","unstructured":"Martin R. G. Gillette W. K. Rosner J. L. Promoter discrimination by the related transcriptional activators MarA and SoxS: differential regulation by differential binding.Mol. Microbiol.352000623634","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4176-4178.1995"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1574-6968.1998.tb13905.x","article-title":"Overexpression of marA, soxS or acrAB produces resistance to triclosan in Escherichia coli","volume":"166","author":"McMurry L. M.","year":"1998","unstructured":"McMurry L. M. Oethinger M. Levy S. B. Overexpression of marA, soxS or acrAB produces resistance to triclosan in Escherichia coli.FEMS Microbiol. Lett.1661998305309","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.8.1773"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.12.2770"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.8.2089"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.306-308.1996"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"3821","DOI":"10.1093\/nar\/27.19.3821","article-title":"Genome-wide expression profiling in Escherichia coli K-12","volume":"27","author":"Richmond C. S.","year":"1999","unstructured":"Richmond C. S. Glasner J. D. Mau R. Jin H. Blattner F. R. Genome-wide expression profiling in Escherichia coli K-12.Nucleic Acids Res.27199938213835","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.20.6262-6269.1994"},{"key":"e_1_3_2_48_2","unstructured":"Sambrook\nJ.\nFritsch\nE. F.\nManiatis\nT.\nMolecular cloning: a laboratory manual\n2nd ed.\n1989\nCold Spring Harbor Laboratory\nCold Spring Harbor N.Y"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.3.530-535.1995"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1093\/genetics\/148.4.1599","article-title":"Mutagenesis and more: umuDC and the Escherichia coli SOS response","volume":"148","author":"Smith B. T.","year":"1998","unstructured":"Smith B. T. Walker G. C. Mutagenesis and more: umuDC and the Escherichia coli SOS response.Genetics148199815991610","journal-title":"Genetics"},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S1369-5274(99)80033-2","article-title":"Oxidative stress","volume":"2","author":"Storz G.","year":"1999","unstructured":"Storz G. Imlay J. A. Oxidative stress.Curr. Opin. Microbiol.21999188194","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1128\/jb.179.6.1857-1866.1997","article-title":"The Salmonella typhimurium mar locus: molecular and genetic analysis and assessment of its requirement for virulence","volume":"179","author":"Sulavick M. C.","year":"1997","unstructured":"Sulavick M. C. Dazer M. Miller P. F. The Salmonella typhimurium mar locus: molecular and genetic analysis and assessment of its requirement for virulence.J. Bacteriol.179199718571866","journal-title":"J. Bacteriol."},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6425-6440.1999"},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/(SICI)1521-1878(199801)20:1<49::AID-BIES8>3.0.CO;2-N","article-title":"Cold shock and adaptation","volume":"20","author":"Thieringer H. A.","year":"1998","unstructured":"Thieringer H. A. Jones P. G. Inouye M. Cold shock and adaptation.Bioessays.2019984957","journal-title":"Bioessays."},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.19.6122-6126.1997"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.15.4508-4514.1996"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.15.4639-4643.1999"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.182.12.3467-3474.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.182.12.3467-3474.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:52:55Z","timestamp":1627581175000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.182.12.3467-3474.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6,15]]},"references-count":56,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2000,6,15]]}},"alternative-id":["10.1128\/JB.182.12.3467-3474.2000"],"URL":"https:\/\/doi.org\/10.1128\/jb.182.12.3467-3474.2000","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,6,15]]},"assertion":[{"value":"1999-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}