{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:58:25Z","timestamp":1790891905190,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2000,7,15]],"date-time":"2000-07-15T00:00:00Z","timestamp":963619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The GacS-GacA two-component signal transduction system, which is highly conserved in gram-negative bacteria, is required for the production of exoenzymes and secondary metabolites in\n            <jats:italic>Pseudomonas<\/jats:italic>\n            spp. Screening of a\n            <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n            F113 gene bank led to the isolation of a previously undefined locus which could restore secondary metabolite production to both\n            <jats:italic>gacS<\/jats:italic>\n            and\n            <jats:italic>gacA<\/jats:italic>\n            mutants of F113. Sequence analysis of this locus demonstrated that it did not contain any obvious\n            <jats:italic>Pseudomonas<\/jats:italic>\n            protein-coding open reading frames or homologues within available databases. Northern analysis indicated that the locus encodes an RNA (PrrB RNA) which is able to phenotypically complement\n            <jats:italic>gacS<\/jats:italic>\n            and\n            <jats:italic>gacA<\/jats:italic>\n            mutants and is itself regulated by the GacS-GacA two-component signal transduction system. Primer extension analysis of the 132-base transcript identified the transcription start site located downstream of a \u03c2\n            <jats:sup>70<\/jats:sup>\n            promoter sequence from positions \u221210 to \u221235. Inactivation of the\n            <jats:italic>prrB<\/jats:italic>\n            gene in F113 resulted in a significant reduction of 2,4-diacetylphloroglucinol (Phl) and hydrogen cyanide (HCN) production, while increased metabolite production was observed when\n            <jats:italic>prrB<\/jats:italic>\n            was overexpressed. The\n            <jats:italic>prrB<\/jats:italic>\n            gene sequence contains a number of imperfect repeats of the consensus sequence 5\u2032-AGGA-3\u2032, and sequence analysis predicted a complex secondary structure featuring multiple putative stem-loops with the consensus sequences predominantly positioned at the single-stranded regions at the ends of the stem-loops. This structure is similar to the CsrB and RsmB regulatory RNAs in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and\n            <jats:italic>Erwinia carotovora<\/jats:italic>\n            , respectively. Results suggest that a regulatory RNA molecule is involved in GacA-GacS-mediated regulation of Phl and HCN production in\n            <jats:italic>P. fluorescens<\/jats:italic>\n            F113.\n          <\/jats:p>","DOI":"10.1128\/jb.182.14.3913-3919.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"3913-3919","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["A Regulatory RNA (PrrB RNA) Modulates Expression of Secondary Metabolite Genes in\n            <i>Pseudomonas fluorescens<\/i>\n            F113"],"prefix":"10.1128","volume":"182","author":[{"given":"Simon","family":"Aarons","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelhamid","family":"Abbas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Adams","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Fenton","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fergal","family":"O'Gara","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment tool","volume":"215","author":"Altschul S. 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