{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:39Z","timestamp":1791942399241,"version":"4.3.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            As part of a\n            <jats:italic>Brucella abortus<\/jats:italic>\n            2308 genome project carried out in our laboratory, we identified, cloned, and sequenced a genomic DNA fragment containing a locus (\n            <jats:italic>virB<\/jats:italic>\n            ) highly homologous to bacterial type IV secretion systems. The\n            <jats:italic>B. abortus virB<\/jats:italic>\n            locus is a collinear arrangement of 13 open reading frames (ORFs). Between\n            <jats:italic>virB1<\/jats:italic>\n            and\n            <jats:italic>virB2<\/jats:italic>\n            and downstream of ORF12, two degenerated, palindromic repeat sequences characteristic of\n            <jats:italic>Brucella<\/jats:italic>\n            intergenic regions were found. Gene reporter studies demonstrated that the\n            <jats:italic>B. abortus virB<\/jats:italic>\n            locus constitutes an operon transcribed from\n            <jats:italic>virB1<\/jats:italic>\n            which is turned on during the stationary phase of growth. A\n            <jats:italic>B. abortus<\/jats:italic>\n            polar\n            <jats:italic>virB1<\/jats:italic>\n            mutant failed to replicate in HeLa cells, indicating that the\n            <jats:italic>virB<\/jats:italic>\n            operon plays a critical role in intracellular multiplication. Mutants with polar and nonpolar mutations introduced in\n            <jats:italic>virB10<\/jats:italic>\n            showed different behaviors in mice and in the HeLa cell infection assay, suggesting that\n            <jats:italic>virB10<\/jats:italic>\n            per se is necessary for the correct function of this type IV secretion apparatus. Mouse infection assays demonstrated that the\n            <jats:italic>virB<\/jats:italic>\n            operon constitutes a major determinant of\n            <jats:italic>B. abortus<\/jats:italic>\n            virulence. It is suggested that putative effector molecules secreted by this type IV secretion system determine routing of\n            <jats:italic>B. abortus<\/jats:italic>\n            to an endoplasmic reticulum-related replication compartment.\n          <\/jats:p>","DOI":"10.1128\/jb.182.17.4849-4855.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:00:58Z","timestamp":1027764058000},"page":"4849-4855","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":226,"title":["A Homologue of an Operon Required for DNA Transfer in\n            <i>Agrobacterium<\/i>\n            Is Required in\n            <i>Brucella abortus<\/i>\n            for Virulence and Intracellular Multiplication"],"prefix":"10.1128","volume":"182","author":[{"given":"Rodrigo","family":"Sieira","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Mart\u0131\u0301n, CONICET, San Mart\u0131\u0301n 1650,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Mart\u0131\u0301n, CONICET, San Mart\u0131\u0301n 1650,1 and"},{"name":"<!--label omitted: 2-->Comisio\u0301n Nacional de Energ\u0131\u0301a Ato\u0301mica, Divisio\u0301n Agropecuaria, Centro Ato\u0301mico Ezeiza 1804,2 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel O.","family":"Sa\u0301nchez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Mart\u0131\u0301n, CONICET, San Mart\u0131\u0301n 1650,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Mart\u0131\u0301n, CONICET, San Mart\u0131\u0301n 1650,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0378-1119(95)00313-U","article-title":"New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions","volume":"162","author":"Becker A.","year":"1995","unstructured":"Becker A. 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